Restriction map for
pF1KA0530
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# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccII AccIII AhdI Alw44I
AocI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbeI BbvCI BglI BlnI BlpI
Bpu1102I BseAI BsiWI BsmBI BsoBI
BspEI BsrBI BssHII BstAPI BstBI
BstUI Bsu36I CelII CpoI DrdI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI FseI FspI KasI
KpnI KspI MluI MluNI MroI
MscI MvnI NarI NcoI NotI
NruI NspV PacI PaeR7I PflFI
PmeI PpuMI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SmaI SphI Sse8387I
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 3279 GACGTC GACGT^C
AclI 6570 AACGTT AA^CGTT
AflII 1816 CTTAAG C^TTAAG
AgeI 2100 ACCGGT A^CCGGT
Aor51HI 758 AGCGCT AGC^GCT
ApaI 2670 GGGCCC GGGCC^C
AseI 6096 ATTAAT AT^TAAT
AsnI 6096 ATTAAT AT^TAAT
BamHI 6487 GGATCC G^GATCC
BanI 3136 GGTGCC G^G[CT][GA]CC
BbrPI 5979 CACGTG CAC^GTG
BcgI 5425 GCAACCCGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5778 GTATCC GTATCC(6/5)
BfrI 1816 CTTAAG C^TTAAG
BsaBI 206 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2100 ACCGGT [AT]^CCGG[AT]
BsiEI 5431 CGGTCG CG[GA][CT]^CG
BspDI 4964 ATCGAT AT^CGAT
BspLU11I 4480 ACATGT A^CATGT
BsrDI 5996 GCAATG GCAATG(2/0)
BssSI 3434 CTCGTG CACGAG(-5/-1)
BstEII 1663 GGTGACC G^GT[ACGT]ACC
BstPI 1663 GGTGACC G^GT[ACGT]ACC
ClaI 4964 ATCGAT AT^CGAT
DraII 2669 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 2789 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 758 AGCGCT AGC^GCT
EcoO109I 2669 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1663 GGTGACC G^GT[ACGT]ACC
EcoT22I 3179 ATGCAT ATGCA^T
FauI 2667 GCGGG CCCGC(4/6)
HaeII 758 AGCGCT [GA]GCGC^[CT]
HgaI 751 GACGC GACGC(5/10)
HindIII 998 AAGCTT A^AGCTT
HpaI 3700 GTTAAC GTT^AAC
MamI 206 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4444 CAATTG C^AATTG
MunI 4444 CAATTG C^AATTG
NaeI 4614 GCCGGC GCC^GGC
NdeI 339 CATATG CA^TATG
NgoMIV 4614 GCCGGC G^CCGGC
NheI 4647 GCTAGC G^CTAGC
NsiI 3179 ATGCAT ATGCA^T
PinAI 2100 ACCGGT A^CCGGT
PmaCI 5979 CACGTG CAC^GTG
PmlI 5979 CACGTG CAC^GTG
PshAI 1367 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6096 ATTAAT AT^TAAT
Psp1406I 6570 AACGTT AA^CGTT
SgrAI 2099 CACCGGTG C[GA]^CCGG[CT]G
SnaBI 1038 TACGTA TAC^GTA
SpeI 6532 ACTAGT A^CTAGT
SwaI 3479 ATTTAAAT ATTT^AAAT
XbaI 5331 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 751 GACGCC G[GA]^CG[CT]C
3279 GACGTC
AvaII 1366 GGACC G^G[AT]CC
3454 GGTCC
BcnI 4515 CCGGG CC^[GC]GG
5429 CCCGG
BsaAI 1038 TACGTA [CT]AC^GT[GA]
5979 CACGTG
BsaHI 751 GACGCC G[GA]^CG[CT]C
3279 GACGTC
BsaI 4705 GAGACC GGTCTC(1/5)
6124 GAGACC
BsmI 5655 GAATGC GAATGC(1/-1)
3116 GCATTC
BspHI 1017 TCATGA T^CATGA
5211 TCATGA
BsrFI 2100 ACCGGT [GA]^CCGG[CT]
4614 GCCGGC
Bst1107I 878 GTATAC GTA^TAC
5386 GTATAC
BstXI 2783 CCAGATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5343 CCACGGGATTGG
BstZ17I 878 GTATAC GTA^TAC
5386 GTATAC
CfoI 759 GCGC GCG^C
2022 GCGC
Cfr10I 2100 ACCGGT [GA]^CCGG[CT]
4614 GCCGGC
DsaI 2661 CCGTGG C^C[GA][CT]GG
5343 CCACGG
EcoRV 4258 GATATC GAT^ATC
6754 GATATC
EcoT14I 276 CCTTGG C^C[AT][AT]GG
4341 CCAAGG
HhaI 759 GCGC GCG^C
2022 GCGC
Hin1I 751 GACGCC G[GA]^CG[CT]C
3279 GACGTC
HinP1I 759 GCGC G^CGC
2022 GCGC
HincII 2220 GTCAAC GT[CT]^[GA]AC
3700 GTTAAC
HindII 2220 GTCAAC GT[CT]^[GA]AC
3700 GTTAAC
NciI 4515 CCGGG CC^[GC]GG
5429 CCCGG
RcaI 1017 TCATGA T^CATGA
5211 TCATGA
SacI 1348 GAGCTC GAGCT^C
6372 GAGCTC
StuI 454 AGGCCT AGG^CCT
4579 AGGCCT
StyI 276 CCTTGG C^C[AT][AT]GG
4341 CCAAGG
XcmI 5172 CCAAGCAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6462 CCATCCGAATACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI BanII BbsI BbvI BclI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
FbaI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI