Restriction map for
pF1KB0054
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AlwI AlwNI AocI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BcnI BglI BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmI BspEI BspHI BspLU11I
BsrFI BssHII BssSI Bsu36I Cfr10I
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
FseI FspI HapII HincII HindII
HpaI HpaII KasI KpnI KspI
MamI MluNI MroI MscI MspI
NaeI NarI NciI NgoMIV NheI
NotI NruI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI Tth111I XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 1226 CGCG CG^CG
AcyI 3 GGCGTC G[GA]^CG[CT]C
AflII 1342 CTTAAG C^TTAAG
Alw44I 751 GTGCAC G^TGCAC
ApaLI 751 GTGCAC G^TGCAC
AspHI 751 GTGCAC G[AT]GC[AT]^C
AvaI 2828 CTCGAG C^[CT]CG[GA]G
AvaII 2789 GGACC G^G[AT]CC
BanII 1749 GAGCCC G[GA]GC[CT]^C
BciVI 1960 GGATAC GTATCC(6/5)
BfrI 1342 CTTAAG C^TTAAG
BlpI 2035 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2035 GCTGAGC GC^T[ACGT]AGC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsiHKAI 751 GTGCAC G[AT]GC[AT]^C
BsoBI 2828 CTCGAG C^[CT]CG[GA]G
Bsp1407I 263 TGTACA T^GTACA
BspMI 464 ACCTGC ACCTGC(4/8)
BsrBI 3019 GAGCGG CCGCTC(-3/-3)
BsrGI 263 TGTACA T^GTACA
BstAPI 1553 GCAACCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2477 GGTTACC G^GT[ACGT]ACC
BstPI 2477 GGTTACC G^GT[ACGT]ACC
BstUI 1226 CGCG CG^CG
CelII 2035 GCTGAGC GC^T[ACGT]AGC
DraII 2788 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 742 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 23 TGGCCG [CT]^GGCC[GA]
EcoNI 2245 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2788 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 2477 GGTTACC G^GT[ACGT]ACC
EcoRI 1865 GAATTC G^AATTC
HaeII 1903 GGCGCT [GA]GCGC^[CT]
Hin1I 3 GGCGTC G[GA]^CG[CT]C
MluI 1225 ACGCGT A^CGCGT
MvnI 1226 CGCG CG^CG
NcoI 2795 CCATGG C^CATGG
NdeI 1595 CATATG CA^TATG
PaeR7I 2828 CTCGAG C^TCGAG
PpuMI 2788 GGGACCC [GA]G^G[AT]CC[CT]
SapI 415 GCTCTTC GCTCTTC(1/4)
SspBI 263 TGTACA T^GTACA
StuI 786 AGGCCT AGG^CCT
XbaI 2928 TCTAGA T^CTAGA
XhoI 2828 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 1225 ACGCGT A^C[GA][CT]GT
2700 ACACGT
BclI 2007 TGATCA T^GATCA
2088 TGATCA
BglII 187 AGATCT A^GATCT
795 AGATCT
BpmI 111 CTGGAG CTGGAG(16/14)
2426 CTGGAG
BseRI 2252 GAGGAG GAGGAG(10/8)
2431 GAGGAG
BspDI 337 ATCGAT AT^CGAT
2131 ATCGAT
Bst1107I 772 GTATAC GTA^TAC
2497 GTATAC
BstBI 246 TTCGAA TT^CGAA
1133 TTCGAA
BstXI 1854 CCATCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2762 CCACAGGGATGG
BstYI 187 AGATCT [GA]^GATC[CT]
795 AGATCT
BstZ17I 772 GTATAC GTA^TAC
2497 GTATAC
CfoI 1904 GCGC GCG^C
1981 GCGC
Cfr13I 2649 GGGCC G^G[ACGT]CC
2789 GGACC
ClaI 337 ATCGAT AT^CGAT
2131 ATCGAT
DsaI 11 CCGTGG C^C[GA][CT]GG
2795 CCATGG
EarI 416 CTCTTC CTCTTC(1/4)
1000 GAAGAG
EcoT22I 311 ATGCAT ATGCA^T
1114 ATGCAT
FauI 376 GCGGG CCCGC(4/6)
819 GCGGG
FbaI 2007 TGATCA T^GATCA
2088 TGATCA
HhaI 1904 GCGC GCG^C
1981 GCGC
HinP1I 1904 GCGC G^CGC
1981 GCGC
HindIII 239 AAGCTT A^AGCTT
401 AAGCTT
Ksp632I 416 CTCTTC CTCTTC(1/4)
1000 GAAGAG
MfeI 293 CAATTG C^AATTG
518 CAATTG
MflI 187 AGATCT [GA]^GATC[CT]
795 AGATCT
MunI 293 CAATTG C^AATTG
518 CAATTG
NsiI 311 ATGCAT ATGCA^T
1114 ATGCAT
NspV 246 TTCGAA TT^CGAA
1133 TTCGAA
PflMI 1753 CCAGACGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1825 CCATGCAATGG
Sau96I 2649 GGGCC G^G[ACGT]CC
2789 GGACC
SfuI 246 TTCGAA TT^CGAA
1133 TTCGAA
SphI 101 GCATGC GCATG^C
1706 GCATGC
SspI 1300 AATATT AAT^ATT
2623 AATATT
Van91I 1753 CCAGACGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1825 CCATGCAATGG
XhoII 187 AGATCT [GA]^GATC[CT]
795 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI AseI AsnI BbsI BbvI
BfaI BmyI BpuAI BsaJI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
PvuII RsaI Sau3AI ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I