Restriction map for
pF1KB3091
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AhdI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbvCI BcgI
BciVI BfrI BglI BlnI BsaHI
BseAI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspMI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstPI
ClaI CpoI DraIII DrdI DsaI
Eam1105I Eco47III EcoO65I EcoT22I FauI
FseI FspI HaeII Hin1I KasI
KpnI KspI MluI MroI MspA1I
NarI NcoI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmeI PshAI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AgeI 725 ACCGGT A^CCGGT
Alw44I 1547 GTGCAC G^TGCAC
AocI 2205 CCTCAGG CC^T[ACGT]AGG
ApaLI 1547 GTGCAC G^TGCAC
Asp700I 394 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 923 GAGCCC G[GA]GC[CT]^C
BbrPI 1828 CACGTG CAC^GTG
BbsI 394 GAAGAC GAAGAC(2/6)
BlpI 1798 GCTCAGC GC^T[ACGT]AGC
BpmI 620 CTGGAG CTGGAG(16/14)
Bpu1102I 1798 GCTCAGC GC^T[ACGT]AGC
BpuAI 394 GAAGAC GAAGAC(2/6)
BsaAI 1828 CACGTG [CT]AC^GT[GA]
BsaI 2425 GAGACC GGTCTC(1/5)
BsaWI 725 ACCGGT [AT]^CCGG[AT]
BsmBI 19 GAGACG CGTCTC(1/5)
BspHI 2352 TCATGA T^CATGA
BspLU11I 2400 ACATGT A^CATGT
BsrDI 2307 CATTGC GCAATG(2/0)
BstAPI 2491 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2205 CCTCAGG CC^T[ACGT]AGG
CelII 1798 GCTCAGC GC^T[ACGT]AGC
DraI 1674 TTTAAA TTT^AAA
DraII 716 AGGACCT [GA]G^G[ACGT]CC[CT]
EagI 41 CGGCCG C^GGCCG
EclXI 41 CGGCCG C^GGCCG
Eco52I 41 CGGCCG C^GGCCG
Eco81I 2205 CCTCAGG CC^T[ACGT]AGG
EcoO109I 716 AGGACCT [GA]G^G[ACGT]CC[CT]
HindIII 1091 AAGCTT A^AGCTT
HpaI 1024 GTTAAC GTT^AAC
MfeI 2152 CAATTG C^AATTG
MunI 2152 CAATTG C^AATTG
NaeI 204 GCCGGC GCC^GGC
NdeI 1853 CATATG CA^TATG
NgoMIV 204 GCCGGC G^CCGGC
PinAI 725 ACCGGT A^CCGGT
PmaCI 1828 CACGTG CAC^GTG
PmlI 1828 CACGTG CAC^GTG
PpuMI 716 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 2352 TCATGA T^CATGA
SmaI 161 CCCGGG CCC^GGG
SspI 1747 AATATT AAT^ATT
XmaCI 161 CCCGGG C^CCGGG
XmaI 161 CCCGGG C^CCGGG
XmnI 394 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1686 GTATAC GT^[AC][GT]AC
1963 GTATAC
AccII 39 CGCG CG^CG
147 CGCG
AflIII 1829 ACGTGT A^C[GA][CT]GT
2400 ACATGT
AlwI 1423 GATCC GGATC(4/5)
1380 GGATC
AlwNI 970 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1255 CAGGCTCTG
AseI 1485 ATTAAT AT^TAAT
2229 ATTAAT
AsnI 1485 ATTAAT AT^TAAT
2229 ATTAAT
AspHI 1547 GTGCAC G[AT]GC[AT]^C
1841 GAGCAC
AvaI 161 CCCGGG C^[CT]CG[GA]G
2129 CCCGAG
BalI 468 TGGCCA TGG^CCA
2369 TGGCCA
BfaI 517 CTAG C^TAG
1804 CTAG
BsgI 7 GTGCAG GTGCAG(16/14)
1493 GTGCAG
BsiEI 41 CGGCCG CG[GA][CT]^CG
111 CGACCG
BsiHKAI 1547 GTGCAC G[AT]GC[AT]^C
1841 GAGCAC
BsoBI 161 CCCGGG C^[CT]CG[GA]G
2129 CCCGAG
BsrFI 204 GCCGGC [GA]^CCGG[CT]
725 ACCGGT
Bst1107I 1686 GTATAC GTA^TAC
1963 GTATAC
BstUI 39 CGCG CG^CG
147 CGCG
BstXI 1632 CCAGAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2168 CCACGTTCATGG
BstZ17I 1686 GTATAC GTA^TAC
1963 GTATAC
Cfr10I 204 GCCGGC [GA]^CCGG[CT]
725 ACCGGT
EcoNI 933 CCTTTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2661 CCTGTCCAAGG
EcoRV 1627 GATATC GAT^ATC
2590 GATATC
HgaI 21 GACGC GACGC(5/10)
145 GACGC
HincII 1024 GTTAAC GT[CT]^[GA]AC
1479 GTCAAC
HindII 1024 GTTAAC GT[CT]^[GA]AC
1479 GTCAAC
MaeI 517 CTAG C^TAG
1804 CTAG
MluNI 468 TGGCCA TGG^CCA
2369 TGGCCA
MscI 468 TGGCCA TGG^CCA
2369 TGGCCA
MvnI 39 CGCG CG^CG
147 CGCG
PshBI 1485 ATTAAT AT^TAAT
2229 ATTAAT
PstI 25 CTGCAG CTGCA^G
223 CTGCAG
ScaI 1618 AGTACT AGT^ACT
2055 AGTACT
SphI 74 GCATGC GCATG^C
1914 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BclI BcnI BglII
BmyI BsaBI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII