##################################
# NO CUT ENZYMES (  212 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BbvI        
BcgI        BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DdeI        DraI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TseI        Tsp45I      Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AfaI          222  GTAC                 GT^AC
BbsI          117  GAAGAC               GAAGAC(2/6)
BpuAI         117  GAAGAC               GAAGAC(2/6)
BsaI          103  GGTCTC               GGTCTC(1/5)
BsmAI         104  GTCTC                GTCTC(1/5)
BstF5I        225  CATCC                GGATG(2/0)
Cac8I          74  GCAAGC               GC[ACGT]^[ACGT]GC
DpnII         243  GATC                 ^GATC
DraII         128  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI           99  CCATGG               C^C[GA][CT]GG
EcoO109I      128  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I        99  CCATGG               C^C[AT][AT]GG
Fnu4HI        151  GCCGC                GC^[ACGT]GC
FokI          225  CATCC                GGATG(9/13)
HinfI         210  GAATC                G^A[ACGT]TC
HphI            4  GGTGA                GGTGA(8/7)
ItaI          151  GCCGC                GC^[ACGT]GC
MboI          243  GATC                 ^GATC
NcoI           99  CCATGG               C^CATGG
NdeII         243  GATC                 ^GATC
NlaIII        100  CATG                 CATG^
NlaIV         129  GGCCCC               GG[ACGT]^[ACGT]CC
RsaI          222  GTAC                 GT^AC
Sau3AI        243  GATC                 ^GATC
SfaNI         170  GCATC                GCATC(5/9)
StyI           99  CCATGG               C^C[AT][AT]GG
TfiI          210  GAATC                G^A[AT]TC
XcmI           99  CCATGGTCTCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AciI          152  CCGC                 CCGC(-3/-1)
              114  GCGG                
AluI          288  AGCT                 AG^CT
              303  AGCT                
BciVI         136  GGATAC               GTATCC(6/5)
              186  GGATAC              
BsrI           85  ACTGG                ACTGG(1/-1)
               48  CCAGT               
BssKI         133  CCTGG                ^CC[ACGT]GG
              229  CCTGG               
BstNI         133  CCTGG                CC^[AT]GG
              229  CCTGG               
Cfr13I         88  GGGCC                G^G[ACGT]CC
              129  GGCCC               
EcoRII        133  CCTGG                ^CC[AT]GG
              229  CCTGG               
HaeIII         89  GGCC                 GG^CC
              129  GGCC                
MnlI           71  GAGG                 CCTC(7/6)
              184  GAGG                
MseI          257  TTAA                 T^TAA
              283  TTAA                
MvaI          133  CCTGG                CC^[AT]GG
              229  CCTGG               
Sau96I         88  GGGCC                G^G[ACGT]CC
              129  GGCCC               
ScrFI         133  CCTGG                CC^[ACGT]GG
              229  CCTGG               
Tru9I         257  TTAA                 T^TAA
              283  TTAA                
TspRI          49  CAGTG                CA[GC]TG[ACGT][ACGT]^
              191  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

BsaJI       MboII