Restriction map for
pF1KB8812
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AflII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
AspI AvaI AviII AvrII BbrPI
BbsI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
CelII ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MaeII MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfaNI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I Tth111I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AhdI 91 GACAAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 769 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 529 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 91 GACAAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 713 TGGCCA TGG^CCA
BamHI 129 GGATCC G^GATCC
BbeI 431 GGCGCC GGCGC^C
BbvCI 619 CCTCAGC CCTCAGC(-5/-2)
BcgI 24 GCAGGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1053 CCCGG CC^[GC]GG
BpmI 597 CTCCAG CTGGAG(16/14)
BsmBI 98 CGTCTC CGTCTC(1/5)
BsmI 35 GCATTC GAATGC(1/-1)
BsrBI 142 GAGCGG CCGCTC(-3/-3)
BsrFI 11 GCCGGT [GA]^CCGG[CT]
BssSI 5 CTCGTG CACGAG(-5/-1)
BstEII 573 GGTTACC G^GT[ACGT]ACC
BstPI 573 GGTTACC G^GT[ACGT]ACC
Cac8I 602 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 432 GCGC GCG^C
Cfr10I 11 GCCGGT [GA]^CCGG[CT]
Eam1105I 91 GACAAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 573 GGTTACC G^GT[ACGT]ACC
HaeII 431 GGCGCC [GA]GCGC^[CT]
HgaI 97 GCGTC GACGC(5/10)
HhaI 432 GCGC GCG^C
HinP1I 432 GCGC G^CGC
HinfI 913 GAGTC G^A[ACGT]TC
HphI 633 GGTGA GGTGA(8/7)
KasI 431 GGCGCC G^GCGCC
MaeIII 574 GTTAC ^GT[ACGT]AC
MluNI 713 TGGCCA TGG^CCA
MscI 713 TGGCCA TGG^CCA
MslI 71 CACATCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 431 GGCGCC GG^CGCC
NciI 1053 CCCGG CC^[GC]GG
PleI 913 GAGTC GAGTC(4/5)
PstI 227 CTGCAG CTGCA^G
SacI 505 GAGCTC GAGCT^C
SfcI 227 CTGCAG C^T[GA][CT]AG
SfoI 431 GGCGCC GGC^GCC
SmlI 523 CTCAAG C^T[CT][GA]AG
StuI 452 AGGCCT AGG^CCT
Tsp509I 58 AATT ^AATT
TspRI 611 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 748 TCTAGA T^CTAGA
XmnI 529 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 294 GGTACC G^GTACC
1027 GGTACC
AcyI 96 GGCGTC G[GA]^CG[CT]C
431 GGCGCC
AflIII 371 ACATGT A^C[GA][CT]GT
829 ACATGT
AocI 426 CCTCAGG CC^T[ACGT]AGG
1022 CCTTAGG
ApaI 27 GGGCCC GGGCC^C
796 GGGCCC
Asp718I 294 GGTACC G^GTACC
1027 GGTACC
BglI 434 GCCTAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
706 GCCGCCATGGC
BsaHI 96 GGCGTC G[GA]^CG[CT]C
431 GGCGCC
BspLU11I 371 ACATGT A^CATGT
829 ACATGT
BspMI 225 ACCTGC ACCTGC(4/8)
482 ACCTGC
BstF5I 73 CATCC GGATG(2/0)
952 CATCC
Bsu36I 426 CCTCAGG CC^T[ACGT]AGG
1022 CCTTAGG
DraIII 162 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
274 CACAGGGTG
EaeI 9 TGGCCG [CT]^GGCC[GA]
713 TGGCCA
Eco81I 426 CCTCAGG CC^T[ACGT]AGG
1022 CCTTAGG
FokI 73 CATCC GGATG(9/13)
952 CATCC
HapII 12 CCGG C^CGG
1054 CCGG
Hin1I 96 GGCGTC G[GA]^CG[CT]C
431 GGCGCC
HpaII 12 CCGG C^CGG
1054 CCGG
KpnI 294 GGTACC GGTAC^C
1027 GGTACC
MspI 12 CCGG C^CGG
1054 CCGG
PflMI 273 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
564 CCATGCTGTGG
PpuMI 237 GGGTCCC [GA]G^G[AT]CC[CT]
479 GGGACCT
SapI 192 GAAGAGC GCTCTTC(1/4)
507 GCTCTTC
TaqI 674 TCGA T^CGA
764 TCGA
TthHB8I 674 TCGA T^CGA
764 TCGA
Van91I 273 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
564 CCATGCTGTGG
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BanII BbvI BfaI
BmyI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstNI BstYI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI HaeIII
ItaI Ksp632I MaeI MboI MboII
MflI MnlI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PvuII RsaI Sau3AI Sau96I ScrFI
StyI TseI XhoII