Restriction map for
pF1KB4148
##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbeI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BspEI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MflI MluI
MroI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PpuMI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I StuI SwaI Van91I
XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccI 967 GTCTAC GT^[AC][GT]AC
AccII 73 CGCG CG^CG
AlwNI 1351 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 930 ATTAAT AT^TAAT
AsnI 930 ATTAAT AT^TAAT
AspHI 863 GAGCTC G[AT]GC[AT]^C
AspI 70 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 303 TGGCCA TGG^CCA
BanI 948 GGCACC G^G[CT][GA]CC
BanII 863 GAGCTC G[GA]GC[CT]^C
BbrPI 1077 CACGTG CAC^GTG
BciVI 143 GGATAC GTATCC(6/5)
BfaI 1325 CTAG C^TAG
BmyI 863 GAGCTC G[AGT]GC[ACT]^C
BpmI 1340 CTGGAG CTGGAG(16/14)
BsaAI 1077 CACGTG [CT]AC^GT[GA]
BseRI 1270 GAGGAG GAGGAG(10/8)
BsiHKAI 863 GAGCTC G[AT]GC[AT]^C
Bsp1286I 863 GAGCTC G[AGT]GC[ACT]^C
BspDI 852 ATCGAT AT^CGAT
BspHI 867 TCATGA T^CATGA
BsrFI 15 GCCGGC [GA]^CCGG[CT]
BstAPI 1350 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 149 CCAGG CC^[AT]GG
BstUI 73 CGCG CG^CG
BstXI 1176 CCAGTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 208 GCGC GCG^C
Cfr10I 15 GCCGGC [GA]^CCGG[CT]
ClaI 852 ATCGAT AT^CGAT
DraI 597 TTTAAA TTT^AAA
DraII 1298 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 70 GACCGCGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1438 GAAGAG CTCTTC(1/4)
EcoO109I 1298 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRII 149 CCAGG ^CC[AT]GG
EcoT14I 300 CCATGG C^C[AT][AT]GG
HgaI 74 GCGTC GACGC(5/10)
HhaI 208 GCGC GCG^C
HinP1I 208 GCGC G^CGC
Ksp632I 1438 GAAGAG CTCTTC(1/4)
MaeI 1325 CTAG C^TAG
MaeII 1078 ACGT A^CGT
MfeI 1232 CAATTG C^AATTG
MluNI 303 TGGCCA TGG^CCA
MscI 303 TGGCCA TGG^CCA
MslI 1311 CACAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1190 CAGCTG C[AC]G^C[GT]G
MunI 1232 CAATTG C^AATTG
MvaI 149 CCAGG CC^[AT]GG
MvnI 73 CGCG CG^CG
NaeI 15 GCCGGC GCC^GGC
NcoI 300 CCATGG C^CATGG
NgoMIV 15 GCCGGC G^CCGGC
NspI 1530 ACATGC [GA]CATG^[CT]
PflFI 70 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1077 CACGTG CAC^GTG
PmlI 1077 CACGTG CAC^GTG
PshBI 930 ATTAAT AT^TAAT
PvuII 1190 CAGCTG CAG^CTG
RcaI 867 TCATGA T^CATGA
SacI 863 GAGCTC GAGCT^C
StyI 300 CCATGG C^C[AT][AT]GG
Tth111I 70 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1039 CCACATCCTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 826 GAATTT [GA]^AATT[CT]
1223 AAATTC
AfaI 897 GTAC GT^AC
1495 GTAC
ApoI 826 GAATTT [GA]^AATT[CT]
1223 AAATTC
AvaI 20 CCCGGG C^[CT]CG[GA]G
61 CCCGGG
AvaII 700 GGACC G^G[AT]CC
722 GGTCC
BsgI 26 CTGCAC GTGCAG(16/14)
590 CTGCAC
BsmFI 1549 GGGAC GGGAC(10/14)
88 GTCCC
BsmI 481 GAATGC GAATGC(1/-1)
1472 GAATGC
BsoBI 20 CCCGGG C^[CT]CG[GA]G
61 CCCGGG
Bsp1407I 896 TGTACA T^GTACA
1494 TGTACA
BspMI 348 ACCTGC ACCTGC(4/8)
690 ACCTGC
BsrGI 896 TGTACA T^GTACA
1494 TGTACA
Cac8I 15 GCCGGC GC[ACGT]^[ACGT]GC
883 GCAGGC
DraIII 172 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
282 CACAGTGTG
DsaI 300 CCATGG C^C[GA][CT]GG
1545 CCACGG
EaeI 2 TGGCCG [CT]^GGCC[GA]
303 TGGCCA
FauI 42 CCCGC CCCGC(4/6)
48 CCCGC
HindIII 554 AAGCTT A^AGCTT
566 AAGCTT
PstI 1193 CTGCAG CTGCA^G
1428 CTGCAG
RsaI 897 GTAC GT^AC
1495 GTAC
SfaNI 880 GATGC GCATC(5/9)
1489 GATGC
SmaI 20 CCCGGG CCC^GGG
61 CCCGGG
SmlI 875 CTCAAG C^T[CT][GA]AG
1294 CTCAAG
SspBI 896 TGTACA T^GTACA
1494 TGTACA
SspI 500 AATATT AAT^ATT
1015 AATATT
XmaCI 20 CCCGGG C^CCGGG
61 CCCGGG
XmaI 20 CCCGGG C^CCGGG
61 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwI BbsI BbvI
BcnI BpuAI BsaJI BsiYI BslI
BsrI BssKI BstF5I Cfr13I DdeI
DpnII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MseI MspI
MwoI NciI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I