Restriction map for
pF1KB8824
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AflIII 282 ACGTGT A^C[GA][CT]GT
AocI 697 CCTGAGG CC^T[ACGT]AGG
ApaI 875 GGGCCC GGGCC^C
AvrII 213 CCTAGG C^CTAGG
BglI 127 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 213 CCTAGG C^CTAGG
BsaAI 281 TACGTG [CT]AC^GT[GA]
BsrFI 797 GCCGGC [GA]^CCGG[CT]
BsrI 843 ACTGG ACTGG(1/-1)
BstAPI 1044 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1113 AGATCC [GA]^GATC[CT]
Bsu36I 697 CCTGAGG CC^T[ACGT]AGG
Cfr10I 797 GCCGGC [GA]^CCGG[CT]
EarI 175 GAAGAG CTCTTC(1/4)
Eco81I 697 CCTGAGG CC^T[ACGT]AGG
HaeII 1091 GGCGCT [GA]GCGC^[CT]
HincII 949 GTCAAC GT[CT]^[GA]AC
HindII 949 GTCAAC GT[CT]^[GA]AC
Ksp632I 175 GAAGAG CTCTTC(1/4)
MaeII 282 ACGT A^CGT
MflI 1113 AGATCC [GA]^GATC[CT]
MseI 773 TTAA T^TAA
NaeI 797 GCCGGC GCC^GGC
NcoI 849 CCATGG C^CATGG
NgoMIV 797 GCCGGC G^CCGGC
PflMI 512 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 88 AGGACCC [GA]G^G[AT]CC[CT]
PstI 586 CTGCAG CTGCA^G
SacI 1138 GAGCTC GAGCT^C
SphI 888 GCATGC GCATG^C
Tru9I 773 TTAA T^TAA
Tsp509I 60 AATT ^AATT
Van91I 512 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 184 CCAGCTGTAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1113 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 303 GATCC GGATC(4/5)
1114 GATCC
AspHI 22 GTGCTC G[AT]GC[AT]^C
1138 GAGCTC
BalI 443 TGGCCA TGG^CCA
764 TGGCCA
BbsI 735 GAAGAC GAAGAC(2/6)
505 GTCTTC
BfaI 214 CTAG C^TAG
1054 CTAG
BpmI 46 CTCCAG CTGGAG(16/14)
1038 CTGGAG
BpuAI 735 GAAGAC GAAGAC(2/6)
505 GTCTTC
BsiHKAI 22 GTGCTC G[AT]GC[AT]^C
1138 GAGCTC
BspMI 584 ACCTGC ACCTGC(4/8)
1157 ACCTGC
CfoI 567 GCGC GCG^C
1092 GCGC
DpnII 303 GATC ^GATC
1114 GATC
DraII 88 AGGACCC [GA]G^G[ACGT]CC[CT]
874 GGGGCCC
DsaI 849 CCATGG C^C[GA][CT]GG
978 CCACGG
EcoO109I 88 AGGACCC [GA]G^G[ACGT]CC[CT]
874 GGGGCCC
FauI 383 CCCGC CCCGC(4/6)
255 GCGGG
HhaI 567 GCGC GCG^C
1092 GCGC
HinP1I 567 GCGC G^CGC
1092 GCGC
MaeI 214 CTAG C^TAG
1054 CTAG
MboI 303 GATC ^GATC
1114 GATC
MluNI 443 TGGCCA TGG^CCA
764 TGGCCA
MscI 443 TGGCCA TGG^CCA
764 TGGCCA
NdeII 303 GATC ^GATC
1114 GATC
PvuII 185 CAGCTG CAG^CTG
1009 CAGCTG
Sau3AI 303 GATC ^GATC
1114 GATC
SfcI 188 CTGTAG C^T[GA][CT]AG
586 CTGCAG
SmlI 616 CTCAAG C^T[CT][GA]AG
648 CTCAAG
StuI 50 AGGCCT AGG^CCT
363 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV NspI
PleI RsaI Sau96I ScrFI SfaNI
StyI TseI Tsp45I TspRI