Restriction map for
pF1KE0403
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BanII BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MboII MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 163 AAATTC [GA]^AATT[CT]
AlwNI 152 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 511 CCTCAGG CC^T[ACGT]AGG
ApoI 163 AAATTC [GA]^AATT[CT]
AvaII 504 GGACC G^G[AT]CC
BamHI 93 GGATCC G^GATCC
BanI 409 GGCACC G^G[CT][GA]CC
BseRI 289 CTCCTC GAGGAG(10/8)
BsmFI 467 GTCCC GGGAC(10/14)
BspHI 314 TCATGA T^CATGA
BsrFI 506 ACCGGC [GA]^CCGG[CT]
BsrI 378 ACTGG ACTGG(1/-1)
BstAPI 229 GCACAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 93 GGATCC [GA]^GATC[CT]
Bsu36I 511 CCTCAGG CC^T[ACGT]AGG
Cac8I 446 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 573 GCGC GCG^C
Cfr10I 506 ACCGGC [GA]^CCGG[CT]
DpnII 94 GATC ^GATC
DraIII 303 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 511 CCTCAGG CC^T[ACGT]AGG
EcoRV 324 GATATC GAT^ATC
FauI 420 GCGGG CCCGC(4/6)
HapII 507 CCGG C^CGG
HgaI 581 GACGC GACGC(5/10)
HhaI 573 GCGC GCG^C
HinP1I 573 GCGC G^CGC
HpaII 507 CCGG C^CGG
MaeII 563 ACGT A^CGT
MboI 94 GATC ^GATC
MflI 93 GGATCC [GA]^GATC[CT]
MslI 606 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 535 CAGCGG C[AC]G^C[GT]G
MspI 507 CCGG C^CGG
NdeII 94 GATC ^GATC
PleI 403 GAGTC GAGTC(4/5)
PstI 78 CTGCAG CTGCA^G
RcaI 314 TCATGA T^CATGA
Sau3AI 94 GATC ^GATC
SfcI 78 CTGCAG C^T[GA][CT]AG
SmlI 634 CTCAAG C^T[CT][GA]AG
TaqI 130 TCGA T^CGA
TfiI 483 GATTC G^A[AT]TC
Tsp509I 164 AATT ^AATT
TthHB8I 130 TCGA T^CGA
XhoII 93 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwI 94 GATCC GGATC(4/5)
93 GGATC
BciVI 386 GTATCC GTATCC(6/5)
530 GTATCC
BsmAI 154 GAGAC GTCTC(1/5)
375 GAGAC
DraII 54 AGGGCCT [GA]G^G[ACGT]CC[CT]
284 AGGGCCT
DsaI 63 CCATGG C^C[GA][CT]GG
470 CCATGG
EcoO109I 54 AGGGCCT [GA]G^G[ACGT]CC[CT]
284 AGGGCCT
HinfI 403 GAGTC G^A[ACGT]TC
483 GATTC
HphI 121 TCACC GGTGA(8/7)
566 TCACC
MaeIII 213 GTCAC ^GT[ACGT]AC
565 GTCAC
NcoI 63 CCATGG C^CATGG
470 CCATGG
Tsp45I 213 GTCAC ^GT[GC]AC
565 GTCAC
XcmI 413 CCATGCTGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
534 CCAGCGGTACAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cfr13I DdeI EcoRII EcoT14I Fnu4HI
FokI HaeIII ItaI MnlI MvaI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI StyI TseI TspRI