Restriction map for
pF1KE0408
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BcgI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII CfoI
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NarI NcoI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 823 GGTACC G^GTACC
AccI 646 GTCTAC GT^[AC][GT]AC
AccII 490 CGCG CG^CG
AcsI 248 AAATTC [GA]^AATT[CT]
AfaI 824 GTAC GT^AC
AlwNI 233 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 238 CCTGAGG CC^T[ACGT]AGG
ApoI 248 AAATTC [GA]^AATT[CT]
Asp700I 699 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 823 GGTACC G^GTACC
BbvCI 100 GCTGAGG CCTCAGC(-5/-2)
BciVI 813 GGATAC GTATCC(6/5)
BcnI 108 CCGGG CC^[GC]GG
BsaWI 588 TCCGGT [AT]^CCGG[AT]
BsgI 604 GTGCAG GTGCAG(16/14)
BsmBI 520 GAGACG CGTCTC(1/5)
BspMI 219 GCAGGT ACCTGC(4/8)
BsrFI 202 GCCGGC [GA]^CCGG[CT]
BssSI 424 CACGAG CACGAG(-5/-1)
BstUI 490 CGCG CG^CG
Bsu36I 238 CCTGAGG CC^T[ACGT]AGG
Cfr10I 202 GCCGGC [GA]^CCGG[CT]
Eco81I 238 CCTGAGG CC^T[ACGT]AGG
HincII 265 GTCAAC GT[CT]^[GA]AC
HindII 265 GTCAAC GT[CT]^[GA]AC
KpnI 823 GGTACC GGTAC^C
MspA1I 910 CAGCGG C[AC]G^C[GT]G
MvnI 490 CGCG CG^CG
NaeI 202 GCCGGC GCC^GGC
NciI 108 CCGGG CC^[GC]GG
NdeI 623 CATATG CA^TATG
NgoMIV 202 GCCGGC G^CCGGC
PleI 331 GAGTC GAGTC(4/5)
PstI 907 CTGCAG CTGCA^G
RsaI 824 GTAC GT^AC
SacI 493 GAGCTC GAGCT^C
TaqI 413 TCGA T^CGA
TfiI 612 GATTC G^A[AT]TC
Tsp509I 249 AATT ^AATT
TthHB8I 413 TCGA T^CGA
XcmI 536 CCACCTTCAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 699 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AspHI 52 GAGCAC G[AT]GC[AT]^C
493 GAGCTC
BanI 222 GGTGCC G^G[CT][GA]CC
823 GGTACC
BsiHKAI 52 GAGCAC G[AT]GC[AT]^C
493 GAGCTC
BsmAI 520 GAGAC GTCTC(1/5)
367 GTCTC
DpnII 411 GATC ^GATC
514 GATC
EarI 31 GAAGAG CTCTTC(1/4)
46 GAAGAG
EcoNI 300 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
942 CCTGCTGGAGG
EcoT14I 259 CCAAGG C^C[AT][AT]GG
818 CCAAGG
HinfI 331 GAGTC G^A[ACGT]TC
612 GATTC
Ksp632I 31 GAAGAG CTCTTC(1/4)
46 GAAGAG
MaeII 602 ACGT A^CGT
731 ACGT
MaeIII 315 GTCAC ^GT[ACGT]AC
592 GTCAC
MboI 411 GATC ^GATC
514 GATC
NdeII 411 GATC ^GATC
514 GATC
Sau3AI 411 GATC ^GATC
514 GATC
StyI 259 CCAAGG C^C[AT][AT]GG
818 CCAAGG
Tsp45I 315 GTCAC ^GT[GC]AC
592 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BmyI BpmI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MboII MnlI
MspI MvaI MwoI NlaIII NlaIV
Sau96I ScrFI SfcI TseI TspRI