Restriction map for
pF1KB6614
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspI AvrII BamHI
BbeI BbrPI BbvCI BciVI BclI
BfaI BfrI BlnI BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KspI MaeI MaeII
MamI MluI MroI MseI MslI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 356 GGTACC G^GTACC
AgeI 1203 ACCGGT A^CCGGT
AhdI 1209 GACCACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 376 GGGCCC GGGCC^C
Asp718I 356 GGTACC G^GTACC
AspEI 1209 GACCACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 904 GAGCTC G[AT]GC[AT]^C
AviII 569 TGCGCA TGC^GCA
BbsI 1119 GAAGAC GAAGAC(2/6)
BcgI 146 GCAGCACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 896 AGATCT A^GATCT
BlpI 951 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 951 GCTCAGC GC^T[ACGT]AGC
BpuAI 1119 GAAGAC GAAGAC(2/6)
BsaWI 1203 ACCGGT [AT]^CCGG[AT]
BsiHKAI 904 GAGCTC G[AT]GC[AT]^C
BspMI 1275 GCAGGT ACCTGC(4/8)
BssSI 664 CACGAG CACGAG(-5/-1)
BstEII 1206 GGTGACC G^GT[ACGT]ACC
BstPI 1206 GGTGACC G^GT[ACGT]ACC
BstXI 693 CCAGGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 951 GCTCAGC GC^T[ACGT]AGC
DsaI 446 CCGTGG C^C[GA][CT]GG
Eam1105I 1209 GACCACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1237 GAAGAG CTCTTC(1/4)
EcoO65I 1206 GGTGACC G^GT[ACGT]ACC
EcoT14I 926 CCTTGG C^C[AT][AT]GG
FspI 569 TGCGCA TGC^GCA
HinfI 1185 GACTC G^A[ACGT]TC
KpnI 356 GGTACC GGTAC^C
Ksp632I 1237 GAAGAG CTCTTC(1/4)
MfeI 410 CAATTG C^AATTG
MunI 410 CAATTG C^AATTG
PinAI 1203 ACCGGT A^CCGGT
PleI 1185 GACTC GAGTC(4/5)
SacI 904 GAGCTC GAGCT^C
SbfI 116 CCTGCAGG CCTGCA^GG
ScaI 398 AGTACT AGT^ACT
SmaI 372 CCCGGG CCC^GGG
SmlI 631 CTCAAG C^T[CT][GA]AG
Sse8387I 116 CCTGCAGG CCTGCA^GG
StuI 923 AGGCCT AGG^CCT
StyI 926 CCTTGG C^C[AT][AT]GG
Tsp509I 411 AATT ^AATT
XmaCI 372 CCCGGG C^CCGGG
XmaI 372 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 529 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1027 CAGGGGCTG
AvaI 154 CTCGGG C^[CT]CG[GA]G
372 CCCGGG
BalI 596 TGGCCA TGG^CCA
857 TGGCCA
BanI 106 GGTGCC G^G[CT][GA]CC
356 GGTACC
BcnI 372 CCCGG CC^[GC]GG
373 CCGGG
BglI 56 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
484 GCCCGTCTGGC
BsgI 877 GTGCAG GTGCAG(16/14)
1009 GTGCAG
BsmAI 520 GAGAC GTCTC(1/5)
988 GAGAC
BsoBI 154 CTCGGG C^[CT]CG[GA]G
372 CCCGGG
BstAPI 77 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
528 GCAGGCCCTGC
EaeI 596 TGGCCA [CT]^GGCC[GA]
857 TGGCCA
HgaI 724 GACGC GACGC(5/10)
1122 GACGC
MluNI 596 TGGCCA TGG^CCA
857 TGGCCA
MscI 596 TGGCCA TGG^CCA
857 TGGCCA
NaeI 86 GCCGGC GCC^GGC
109 GCCGGC
NciI 372 CCCGG CC^[GC]GG
373 CCGGG
NgoMIV 86 GCCGGC G^CCGGC
109 GCCGGC
TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^
867 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanII BbvI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I XcmI XhoII