Restriction map for
pF1KB7967
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AcyI
AflII AflIII AhdI AlwI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT14I EcoT22I FbaI FokI
FseI Hin1I HindIII KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 453 TCCGGA T^CCGGA
AclI 119 AACGTT AA^CGTT
AcsI 166 AAATTT [GA]^AATT[CT]
AfaI 90 GTAC GT^AC
AgeI 599 ACCGGT A^CCGGT
ApoI 166 AAATTT [GA]^AATT[CT]
AvaII 191 GGACC G^G[AT]CC
AviII 524 TGCGCA TGC^GCA
BfaI 109 CTAG C^TAG
BglII 183 AGATCT A^GATCT
BseAI 453 TCCGGA T^CCGGA
BsmBI 77 CGTCTC CGTCTC(1/5)
BsmFI 532 GGGAC GGGAC(10/14)
BspEI 453 TCCGGA T^CCGGA
BspMI 103 ACCTGC ACCTGC(4/8)
BssSI 49 CACGAG CACGAG(-5/-1)
BstYI 183 AGATCT [GA]^GATC[CT]
DraI 778 TTTAAA TTT^AAA
DsaI 362 CCACGG C^C[GA][CT]GG
EaeI 365 CGGCCA [CT]^GGCC[GA]
EarI 837 GAAGAG CTCTTC(1/4)
EcoRV 240 GATATC GAT^ATC
FauI 530 GCGGG CCCGC(4/6)
FspI 524 TGCGCA TGC^GCA
HaeII 69 GGCGCT [GA]GCGC^[CT]
HgaI 666 GACGC GACGC(5/10)
HincII 738 GTTAAC GT[CT]^[GA]AC
HindII 738 GTTAAC GT[CT]^[GA]AC
HpaI 738 GTTAAC GTT^AAC
Ksp632I 837 GAAGAG CTCTTC(1/4)
MaeI 109 CTAG C^TAG
MflI 183 AGATCT [GA]^GATC[CT]
MroI 453 TCCGGA T^CCGGA
PinAI 599 ACCGGT A^CCGGT
Psp1406I 119 AACGTT AA^CGTT
PstI 618 CTGCAG CTGCA^G
RsaI 90 GTAC GT^AC
ScaI 89 AGTACT AGT^ACT
SfcI 618 CTGCAG C^T[GA][CT]AG
SgrAI 598 CACCGGTG C[GA]^CCGG[CT]G
SmaI 496 CCCGGG CCC^GGG
TspRI 790 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 194 CCAAAATCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 183 AGATCT [GA]^GATC[CT]
XmaCI 496 CCCGGG C^CCGGG
XmaI 496 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 530 GCGG CCGC(-3/-1)
663 GCGG
Alw44I 46 GTGCAC G^TGCAC
595 GTGCAC
ApaLI 46 GTGCAC G^TGCAC
595 GTGCAC
BanI 602 GGTGCC G^G[CT][GA]CC
842 GGCACC
BsaI 52 GAGACC GGTCTC(1/5)
298 GAGACC
BsaWI 453 TCCGGA [AT]^CCGG[AT]
599 ACCGGT
BsgI 829 CTGCAC GTGCAG(16/14)
18 GTGCAG
BsrFI 55 ACCGGC [GA]^CCGG[CT]
599 ACCGGT
BsrI 92 ACTGG ACTGG(1/-1)
248 CCAGT
Cac8I 134 GCGAGC GC[ACGT]^[ACGT]GC
491 GCGTGC
CfoI 70 GCGC GCG^C
525 GCGC
Cfr10I 55 ACCGGC [GA]^CCGG[CT]
599 ACCGGT
DpnII 42 GATC ^GATC
184 GATC
HhaI 70 GCGC GCG^C
525 GCGC
HinP1I 70 GCGC G^CGC
525 GCGC
HinfI 226 GAATC G^A[ACGT]TC
331 GAATC
HphI 550 GGTGA GGTGA(8/7)
431 TCACC
MaeII 5 ACGT A^CGT
120 ACGT
MboI 42 GATC ^GATC
184 GATC
MspA1I 528 CAGCGG C[AC]G^C[GT]G
661 CAGCGG
NdeII 42 GATC ^GATC
184 GATC
SacI 34 GAGCTC GAGCT^C
420 GAGCTC
Sau3AI 42 GATC ^GATC
184 GATC
TfiI 226 GAATC G^A[AT]TC
331 GAATC
Tsp509I 167 AATT ^AATT
416 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AluI AspHI AvaI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BssKI BstNI Cfr13I DdeI
EcoRII Fnu4HI HaeIII HapII HpaII
ItaI MaeIII MboII MnlI MseI
MspI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SfaNI Tru9I
TseI Tsp45I