Restriction map for
pF1KB9947
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AvrII
BamHI BcgI BciVI BfrI BlnI
BlpI Bpu1102I BsaBI BseAI BseRI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI HincII HindII
HpaI KpnI Ksp632I KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 86 ACATGT A^C[GA][CT]GT
AhdI 1374 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 912 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1374 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 82 GAGCAC G[AT]GC[AT]^C
AspI 1426 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 364 GGGCTC G[GA]GC[CT]^C
BbrPI 1337 CACGTG CAC^GTG
BbsI 916 GTCTTC GAAGAC(2/6)
BbvCI 1447 GCTGAGG CCTCAGC(-5/-2)
BclI 1001 TGATCA T^GATCA
BglII 518 AGATCT A^GATCT
BpuAI 916 GTCTTC GAAGAC(2/6)
BsaI 256 GAGACC GGTCTC(1/5)
BsaWI 1537 ACCGGA [AT]^CCGG[AT]
BsgI 52 CTGCAC GTGCAG(16/14)
BsiHKAI 82 GAGCAC G[AT]GC[AT]^C
BsmBI 175 GAGACG CGTCTC(1/5)
BspLU11I 86 ACATGT A^CATGT
BspMI 1304 GCAGGT ACCTGC(4/8)
BsrDI 1350 CATTGC GCAATG(2/0)
BsrFI 314 ACCGGC [GA]^CCGG[CT]
BssSI 878 CTCGTG CACGAG(-5/-1)
BstAPI 1153 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1232 CCACCGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 314 ACCGGC [GA]^CCGG[CT]
DraIII 1315 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1374 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 1013 CCCGC CCCGC(4/6)
FbaI 1001 TGATCA T^GATCA
HgaI 724 GCGTC GACGC(5/10)
HindIII 288 AAGCTT A^AGCTT
MseI 194 TTAA T^TAA
NcoI 62 CCATGG C^CATGG
PflFI 1426 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1337 CACGTG CAC^GTG
PmlI 1337 CACGTG CAC^GTG
PvuII 49 CAGCTG CAG^CTG
SexAI 1316 ACCAGGT A^CC[AT]GGT
SphI 1127 GCATGC GCATG^C
Tru9I 194 TTAA T^TAA
Tsp509I 1178 AATT ^AATT
Tth111I 1426 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 333 TCTAGA T^CTAGA
XmnI 912 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 1203 GGCGCC G[GA]^CG[CT]C
1544 GGCGCC
AvaI 1022 CTCGGG C^[CT]CG[GA]G
1496 CCCGAG
AviII 272 TGCGCA TGC^GCA
1124 TGCGCA
BalI 1043 TGGCCA TGG^CCA
1322 TGGCCA
BbeI 1203 GGCGCC GGCGC^C
1544 GGCGCC
BfaI 334 CTAG C^TAG
889 CTAG
BglI 1144 GCCCGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1246 GCCGTGGAGGC
BsaAI 523 TACGTG [CT]AC^GT[GA]
1337 CACGTG
BsaHI 1203 GGCGCC G[GA]^CG[CT]C
1544 GGCGCC
BsmAI 175 GAGAC GTCTC(1/5)
256 GAGAC
BsmFI 25 GGGAC GGGAC(10/14)
1103 GGGAC
BsoBI 1022 CTCGGG C^[CT]CG[GA]G
1496 CCCGAG
BspHI 240 TCATGA T^CATGA
819 TCATGA
EaeI 1043 TGGCCA [CT]^GGCC[GA]
1322 TGGCCA
EcoT14I 62 CCATGG C^C[AT][AT]GG
605 CCTTGG
FspI 272 TGCGCA TGC^GCA
1124 TGCGCA
HaeII 1203 GGCGCC [GA]GCGC^[CT]
1544 GGCGCC
Hin1I 1203 GGCGCC G[GA]^CG[CT]C
1544 GGCGCC
KasI 1203 GGCGCC G^GCGCC
1544 GGCGCC
MaeI 334 CTAG C^TAG
889 CTAG
MluNI 1043 TGGCCA TGG^CCA
1322 TGGCCA
MscI 1043 TGGCCA TGG^CCA
1322 TGGCCA
MslI 1233 CACCGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1350 CATTGCTGTG
MspA1I 49 CAGCTG C[AC]G^C[GT]G
378 CAGCGG
NarI 1203 GGCGCC GG^CGCC
1544 GGCGCC
NspI 86 ACATGT [GA]CATG^[CT]
1127 GCATGC
RcaI 240 TCATGA T^CATGA
819 TCATGA
SfoI 1203 GGCGCC GGC^GCC
1544 GGCGCC
StuI 1253 AGGCCT AGG^CCT
1439 AGGCCT
StyI 62 CCATGG C^C[AT][AT]GG
605 CCTTGG
XcmI 1046 CCAATGCAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1389 CCACCTGTACCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XhoII