Restriction map for
pF1KE2473
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BbrPI BbvCI BclI
BfrI BglII BlnI BsaBI BseAI
BsiWI BsmI BspDI BspEI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eam1105I Eco81I
EcoRI EcoRV EcoT22I FbaI HincII
HindII HindIII HpaI KpnI KspI
MamI MluI MroI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 708 GTCTAC GT^[AC][GT]AC
Alw44I 1783 GTGCAC G^TGCAC
ApaLI 1783 GTGCAC G^TGCAC
BamHI 1001 GGATCC G^GATCC
BcgI 2327 GCACCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2271 GGATAC GTATCC(6/5)
BfaI 115 CTAG C^TAG
BsaAI 217 TACGTG [CT]AC^GT[GA]
BsaWI 1985 TCCGGT [AT]^CCGG[AT]
BsiEI 2058 CGGCCG CG[GA][CT]^CG
Bsp1407I 1619 TGTACA T^GTACA
BspHI 654 TCATGA T^CATGA
BsrGI 1619 TGTACA T^GTACA
BstAPI 14 GCAACTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1968 GGTGACC G^GT[ACGT]ACC
BstPI 1968 GGTGACC G^GT[ACGT]ACC
DraIII 1293 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2058 CGGCCG C^GGCCG
EclXI 2058 CGGCCG C^GGCCG
Eco52I 2058 CGGCCG C^GGCCG
EcoO65I 1968 GGTGACC G^GT[ACGT]ACC
MaeI 115 CTAG C^TAG
MfeI 1632 CAATTG C^AATTG
MseI 2027 TTAA T^TAA
MunI 1632 CAATTG C^AATTG
NcoI 2516 CCATGG C^CATGG
PaeR7I 764 CTCGAG C^TCGAG
PpuMI 1724 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 654 TCATGA T^CATGA
SacI 1063 GAGCTC GAGCT^C
SfiI 268 GGCCTGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 1619 TGTACA T^GTACA
StuI 1976 AGGCCT AGG^CCT
TfiI 178 GATTC G^A[AT]TC
Tru9I 2027 TTAA T^TAA
XhoI 764 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 37 enzymes) #
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AlwNI 400 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1501 CAGAACCTG
Aor51HI 1471 AGCGCT AGC^GCT
2265 AGCGCT
AspI 623 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2014 GACACTGTC
AviII 986 TGCGCA TGC^GCA
1546 TGCGCA
BalI 236 TGGCCA TGG^CCA
2306 TGGCCA
BbeI 352 GGCGCC GGCGC^C
1088 GGCGCC
BbsI 2497 GAAGAC GAAGAC(2/6)
928 GTCTTC
BlpI 1149 GCTCAGC GC^T[ACGT]AGC
2178 GCTGAGC
Bpu1102I 1149 GCTCAGC GC^T[ACGT]AGC
2178 GCTGAGC
BpuAI 2497 GAAGAC GAAGAC(2/6)
928 GTCTTC
BsgI 1057 CTGCAC GTGCAG(16/14)
1009 GTGCAG
BsmBI 1022 CGTCTC CGTCTC(1/5)
1381 GAGACG
BspLU11I 1688 ACATGT A^CATGT
2459 ACATGT
BstYI 1001 GGATCC [GA]^GATC[CT]
2378 AGATCC
CelII 1149 GCTCAGC GC^T[ACGT]AGC
2178 GCTGAGC
DsaI 38 CCGTGG C^C[GA][CT]GG
2516 CCATGG
Eco47III 1471 AGCGCT AGC^GCT
2265 AGCGCT
EcoNI 1646 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1732 CCTGGAGCAGG
FseI 195 GGCCGGCC GGCCGG^CC
264 GGCCGGCC
FspI 986 TGCGCA TGC^GCA
1546 TGCGCA
KasI 352 GGCGCC G^GCGCC
1088 GGCGCC
MflI 1001 GGATCC [GA]^GATC[CT]
2378 AGATCC
MluNI 236 TGGCCA TGG^CCA
2306 TGGCCA
MscI 236 TGGCCA TGG^CCA
2306 TGGCCA
NarI 352 GGCGCC GG^CGCC
1088 GGCGCC
PflFI 623 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2014 GACACTGTC
PflMI 666 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1500 CCAGAACCTGG
SapI 1884 GAAGAGC GCTCTTC(1/4)
139 GCTCTTC
SexAI 1157 ACCTGGT A^CC[AT]GGT
1505 ACCTGGT
SfoI 352 GGCGCC GGC^GCC
1088 GGCGCC
SmaI 293 CCCGGG CCC^GGG
1346 CCCGGG
Tth111I 623 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2014 GACACTGTC
Van91I 666 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1500 CCAGAACCTGG
XcmI 511 CCACAGTGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2330 CCACCATCGTAGTGG
XhoII 1001 GGATCC [GA]^GATC[CT]
2378 AGATCC
XmaCI 293 CCCGGG C^CCGGG
1346 CCCGGG
XmaI 293 CCCGGG C^CCGGG
1346 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI ApaI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I