Restriction map for
pF1KE1328
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
AlwNI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbeI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI DraIII DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
Hin1I HpaI KasI Ksp632I KspI
MaeIII MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tsp45I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 644 GGTACC G^GTACC
Alw44I 528 GTGCAC G^TGCAC
AocI 188 CCTCAGG CC^T[ACGT]AGG
ApaLI 528 GTGCAC G^TGCAC
Asp718I 644 GGTACC G^GTACC
AvaI 421 CTCGGG C^[CT]CG[GA]G
BbrPI 69 CACGTG CAC^GTG
BbvCI 967 GCTGAGG CCTCAGC(-5/-2)
BfaI 631 CTAG C^TAG
BlpI 393 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 393 GCTCAGC GC^T[ACGT]AGC
BsaAI 69 CACGTG [CT]AC^GT[GA]
BsmFI 1011 GTCCC GGGAC(10/14)
BsoBI 421 CTCGGG C^[CT]CG[GA]G
BstYI 6 GGATCT [GA]^GATC[CT]
Bsu36I 188 CCTCAGG CC^T[ACGT]AGG
CelII 393 GCTCAGC GC^T[ACGT]AGC
DraI 832 TTTAAA TTT^AAA
DraII 1009 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 229 GACCTGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 188 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1009 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 167 CCAAGG C^C[AT][AT]GG
HaeII 599 AGCGCC [GA]GCGC^[CT]
HgaI 223 GACGC GACGC(5/10)
HincII 474 GTTGAC GT[CT]^[GA]AC
HindII 474 GTTGAC GT[CT]^[GA]AC
HindIII 664 AAGCTT A^AGCTT
HinfI 1029 GATTC G^A[ACGT]TC
KpnI 644 GGTACC GGTAC^C
MaeI 631 CTAG C^TAG
MaeII 70 ACGT A^CGT
MflI 6 GGATCT [GA]^GATC[CT]
NspI 80 GCATGC [GA]CATG^[CT]
PmaCI 69 CACGTG CAC^GTG
PmlI 69 CACGTG CAC^GTG
PpuMI 1009 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 538 CTGCAG CTGCA^G
SexAI 230 ACCTGGT A^CC[AT]GGT
SmlI 613 CTCAAG C^T[CT][GA]AG
SphI 80 GCATGC GCATG^C
StyI 167 CCAAGG C^C[AT][AT]GG
TfiI 1029 GATTC G^A[AT]TC
XcmI 1015 CCAGTGAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 6 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 68 ACACGT A^C[GA][CT]GT
70 ACGTGT
BanI 199 GGCACC G^G[CT][GA]CC
644 GGTACC
BanII 697 GAGCTC G[GA]GC[CT]^C
1020 GAGCTC
BbsI 49 GAAGAC GAAGAC(2/6)
238 GTCTTC
BcnI 282 CCGGG CC^[GC]GG
900 CCCGG
BpmI 415 CTCCAG CTGGAG(16/14)
694 CTGGAG
BpuAI 49 GAAGAC GAAGAC(2/6)
238 GTCTTC
BsaI 932 GAGACC GGTCTC(1/5)
1041 GAGACC
BseRI 186 CTCCTC GAGGAG(10/8)
523 GAGGAG
BspMI 536 ACCTGC ACCTGC(4/8)
562 ACCTGC
BstXI 26 CCAAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
937 CCAAAGGGTTGG
CfoI 391 GCGC GCG^C
600 GCGC
HapII 282 CCGG C^CGG
901 CCGG
HhaI 391 GCGC GCG^C
600 GCGC
HinP1I 391 GCGC G^CGC
600 GCGC
HpaII 282 CCGG C^CGG
901 CCGG
HphI 1038 GGTGA GGTGA(8/7)
254 TCACC
MspA1I 313 CAGCTG C[AC]G^C[GT]G
952 CAGCTG
MspI 282 CCGG C^CGG
901 CCGG
NciI 282 CCGGG CC^[GC]GG
900 CCCGG
PvuII 313 CAGCTG CAG^CTG
952 CAGCTG
SacI 697 GAGCTC GAGCT^C
1020 GAGCTC
SfaNI 460 GATGC GCATC(5/9)
806 GCATC
StuI 883 AGGCCT AGG^CCT
971 AGGCCT
TaqI 440 TCGA T^CGA
521 TCGA
TspRI 640 CACTG CA[GC]TG[ACGT][ACGT]^
1016 CAGTG
TthHB8I 440 TCGA T^CGA
521 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII ItaI MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfcI Tru9I TseI Tsp509I