Restriction map for
pF1KE1327
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BbeI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MroI MseI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Acc65I 956 GGTACC G^GTACC
AcsI 703 AAATTT [GA]^AATT[CT]
ApoI 703 AAATTT [GA]^AATT[CT]
Asp718I 956 GGTACC G^GTACC
AspI 728 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1043 GGATCC G^GATCC
BanI 956 GGTACC G^G[CT][GA]CC
BbrPI 770 CACGTG CAC^GTG
BbsI 791 GAAGAC GAAGAC(2/6)
BbvCI 1317 GCTGAGG CCTCAGC(-5/-2)
BcgI 1139 CGATGCGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1039 CTAG C^TAG
BglII 509 AGATCT A^GATCT
BpuAI 791 GAAGAC GAAGAC(2/6)
BsaI 903 GAGACC GGTCTC(1/5)
BsaWI 529 TCCGGT [AT]^CCGG[AT]
BspLU11I 714 ACATGT A^CATGT
BsrDI 1334 CATTGC GCAATG(2/0)
BstF5I 387 GGATG GGATG(2/0)
DraIII 25 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 80 GACTCGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI 387 GGATG GGATG(9/13)
HgaI 1143 GCGTC GACGC(5/10)
KpnI 956 GGTACC GGTAC^C
MaeI 1039 CTAG C^TAG
NspI 714 ACATGT [GA]CATG^[CT]
PflFI 728 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 770 CACGTG CAC^GTG
PmlI 770 CACGTG CAC^GTG
SacI 822 GAGCTC GAGCT^C
SmlI 1402 CTTGAG C^T[CT][GA]AG
SnaBI 1285 TACGTA TAC^GTA
StuI 9 AGGCCT AGG^CCT
TfiI 963 GAATC G^A[AT]TC
Tth111I 728 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 883 GTAC GT^AC
957 GTAC
AflIII 714 ACATGT A^C[GA][CT]GT
769 ACACGT
AlwI 1044 GATCC GGATC(4/5)
1043 GGATC
ApaI 17 GGGCCC GGGCC^C
639 GGGCCC
AspHI 822 GAGCTC G[AT]GC[AT]^C
876 GAGCAC
AvaI 82 CTCGGG C^[CT]CG[GA]G
1309 CCCGAG
BglI 338 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1006 GCCATTGGGGC
BsaAI 770 CACGTG [CT]AC^GT[GA]
1285 TACGTA
BseRI 869 GAGGAG GAGGAG(10/8)
1208 GAGGAG
BsiHKAI 822 GAGCTC G[AT]GC[AT]^C
876 GAGCAC
BsoBI 82 CTCGGG C^[CT]CG[GA]G
1309 CCCGAG
BspHI 90 TCATGA T^CATGA
1379 TCATGA
BssSI 202 CACGAG CACGAG(-5/-1)
1084 CACGAG
BstAPI 1134 GCAGGCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1231 GCAGGTGGTGC
BstYI 509 AGATCT [GA]^GATC[CT]
1043 GGATCC
Cac8I 1134 GCAGGC GC[ACGT]^[ACGT]GC
1177 GCAGGC
EarI 457 CTCTTC CTCTTC(1/4)
861 GAAGAG
HphI 591 TCACC GGTGA(8/7)
1330 TCACC
Ksp632I 457 CTCTTC CTCTTC(1/4)
861 GAAGAG
MaeII 771 ACGT A^CGT
1286 ACGT
MaeIII 394 GTCAC ^GT[ACGT]AC
734 GTCAC
MflI 509 AGATCT [GA]^GATC[CT]
1043 GGATCC
PflMI 24 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
594 CCACACTCTGG
PstI 1122 CTGCAG CTGCA^G
1268 CTGCAG
RcaI 90 TCATGA T^CATGA
1379 TCATGA
RsaI 883 GTAC GT^AC
957 GTAC
SfcI 1122 CTGCAG C^T[GA][CT]AG
1268 CTGCAG
TaqI 478 TCGA T^CGA
1023 TCGA
Tsp45I 394 GTCAC ^GT[GC]AC
734 GTCAC
TthHB8I 478 TCGA T^CGA
1023 TCGA
Van91I 24 CCACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
594 CCACACTCTGG
XhoII 509 AGATCT [GA]^GATC[CT]
1043 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AluI AlwNI AvaII BalI BanII
BbvI BcnI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstNI
BstXI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MboI MboII MluNI MnlI
MscI MslI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp509I TspRI
XcmI