Restriction map for
pF1KB5454
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI Alw44I AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspHI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaWI BseAI BsiHKAI BsiWI BsmI
BspDI BspEI BspLU11I BssHII BssSI
Bst1107I BstAPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eco81I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NarI NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SgrAI SmlI SnaBI SphI
Sse8387I SwaI Tru9I Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AatII 1495 GACGTC GACGT^C
AccI 891 GTCTAC GT^[AC][GT]AC
AccII 354 CGCG CG^CG
AcyI 1495 GACGTC G[GA]^CG[CT]C
AhdI 1000 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1057 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 74 AGCGCT AGC^GCT
AspEI 1000 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 87 CCCGGG C^[CT]CG[GA]G
BcgI 182 GCAAGACAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 556 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 717 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 717 GCTCAGC GC^T[ACGT]AGC
BsaHI 1495 GACGTC G[GA]^CG[CT]C
BsaI 1176 GGTCTC GGTCTC(1/5)
BsiEI 856 CGGCCG CG[GA][CT]^CG
BsoBI 87 CCCGGG C^[CT]CG[GA]G
Bsp1407I 484 TGTACA T^GTACA
BspHI 683 TCATGA T^CATGA
BsrDI 825 CATTGC GCAATG(2/0)
BsrFI 1211 GCCGGC [GA]^CCGG[CT]
BsrGI 484 TGTACA T^GTACA
BstBI 1217 TTCGAA TT^CGAA
BstEII 1530 GGTCACC G^GT[ACGT]ACC
BstPI 1530 GGTCACC G^GT[ACGT]ACC
BstUI 354 CGCG CG^CG
BstYI 9 GGATCT [GA]^GATC[CT]
CelII 717 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1211 GCCGGC [GA]^CCGG[CT]
DraIII 1078 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 856 CGGCCG C^GGCCG
Eam1105I 1000 GACAACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1024 GAAGAG CTCTTC(1/4)
EclXI 856 CGGCCG C^GGCCG
Eco47III 74 AGCGCT AGC^GCT
Eco52I 856 CGGCCG C^GGCCG
EcoNI 606 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1530 GGTCACC G^GT[ACGT]ACC
HaeII 74 AGCGCT [GA]GCGC^[CT]
Hin1I 1495 GACGTC G[GA]^CG[CT]C
HindIII 1126 AAGCTT A^AGCTT
Ksp632I 1024 GAAGAG CTCTTC(1/4)
MaeII 1496 ACGT A^CGT
MflI 9 GGATCT [GA]^GATC[CT]
MspA1I 103 CAGCTG C[AC]G^C[GT]G
MvnI 354 CGCG CG^CG
NaeI 1211 GCCGGC GCC^GGC
NcoI 921 CCATGG C^CATGG
NdeI 1270 CATATG CA^TATG
NgoMIV 1211 GCCGGC G^CCGGC
NspV 1217 TTCGAA TT^CGAA
PflMI 1056 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 103 CAGCTG CAG^CTG
RcaI 683 TCATGA T^CATGA
ScaI 500 AGTACT AGT^ACT
SexAI 515 ACCTGGT A^CC[AT]GGT
SfuI 1217 TTCGAA TT^CGAA
SmaI 87 CCCGGG CCC^GGG
SpeI 93 ACTAGT A^CTAGT
SspBI 484 TGTACA T^GTACA
SspI 1087 AATATT AAT^ATT
StuI 1139 AGGCCT AGG^CCT
Van91I 1056 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1238 CCAAGAAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 9 GGATCT [GA]^GATC[CT]
XmaCI 87 CCCGGG C^CCGGG
XmaI 87 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 1401 GATCC GGATC(4/5)
9 GGATC
Asp700I 72 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1368 GAAGGAGTTC
BanII 915 GGGCTC G[GA]GC[CT]^C
1152 GGGCTC
BfaI 94 CTAG C^TAG
1504 CTAG
BmyI 915 GGGCTC G[AGT]GC[ACT]^C
1152 GGGCTC
BpmI 1135 CTGGAG CTGGAG(16/14)
1321 CTGGAG
BseRI 322 CTCCTC GAGGAG(10/8)
1045 GAGGAG
BsgI 415 CTGCAC GTGCAG(16/14)
835 CTGCAC
BsmBI 61 GAGACG CGTCTC(1/5)
1493 GAGACG
Bsp1286I 915 GGGCTC G[AGT]GC[ACT]^C
1152 GGGCTC
BspMI 866 ACCTGC ACCTGC(4/8)
895 ACCTGC
BsrBI 358 GAGCGG CCGCTC(-3/-3)
379 GAGCGG
DpnII 10 GATC ^GATC
1401 GATC
EaeI 856 CGGCCG [CT]^GGCC[GA]
924 TGGCCG
EcoT14I 641 CCTTGG C^C[AT][AT]GG
921 CCATGG
MaeI 94 CTAG C^TAG
1504 CTAG
MboI 10 GATC ^GATC
1401 GATC
NdeII 10 GATC ^GATC
1401 GATC
PstI 272 CTGCAG CTGCA^G
1516 CTGCAG
Sau3AI 10 GATC ^GATC
1401 GATC
SfcI 272 CTGCAG C^T[GA][CT]AG
1516 CTGCAG
StyI 641 CCTTGG C^C[AT][AT]GG
921 CCATGG
Tsp509I 1189 AATT ^AATT
1364 AATT
XmnI 72 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1368 GAAGGAGTTC
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BsaJI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboII MnlI MslI MspI MvaI
MwoI NciI NlaIII NlaIV PleI
PpuMI RsaI Sau96I ScrFI SfaNI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I