##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraIII      DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcsI          502  GAATTT               [GA]^AATT[CT]
ApoI          502  GAATTT               [GA]^AATT[CT]
Asp700I       571  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         600  GAGCAC               G[AT]GC[AT]^C
AvaII          75  GGACC                G^G[AT]CC
BclI          467  TGATCA               T^GATCA
BglII         848  AGATCT               A^GATCT
BmyI          600  GAGCAC               G[AGT]GC[ACT]^C
BpmI          545  CTGGAG               CTGGAG(16/14)
BsaJI          80  CCCAGG               C^C[ACGT][ACGT]GG
BseRI          94  GAGGAG               GAGGAG(10/8)
BsgI          329  GTGCAG               GTGCAG(16/14)
BsiHKAI       600  GAGCAC               G[AT]GC[AT]^C
BsiYI          78  CCCCCAGGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           78  CCCCCAGGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         210  GTCTC                GTCTC(1/5)
Bsp1286I      600  GAGCAC               G[AGT]GC[ACT]^C
BssKI          81  CCAGG                ^CC[ACGT]GG
BstAPI         16  GCAGGAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI          81  CCAGG                CC^[AT]GG
BstYI         848  AGATCT               [GA]^GATC[CT]
Cfr13I         75  GGACC                G^G[ACGT]CC
DraII          74  AGGACCC              [GA]G^G[ACGT]CC[CT]
DrdI          402  GACTCATCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I       74  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRII         81  CCAGG                ^CC[AT]GG
FbaI          467  TGATCA               T^GATCA
HphI          778  GGTGA                GGTGA(8/7)
MaeIII        161  GTCAC                ^GT[ACGT]AC
MflI          848  AGATCT               [GA]^GATC[CT]
MslI          237  CACTGCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI           81  CCAGG                CC^[AT]GG
NlaIV          75  GGACCC               GG[ACGT]^[ACGT]CC
PpuMI          74  AGGACCC              [GA]G^G[AT]CC[CT]
PstI           11  CTGCAG               CTGCA^G
Sau96I         75  GGACC                G^G[ACGT]CC
ScaI          612  AGTACT               AGT^ACT
ScrFI          81  CCAGG                CC^[ACGT]GG
SfcI           11  CTGCAG               C^T[GA][CT]AG
SmlI          446  CTCAAG               C^T[CT][GA]AG
TaqI           68  TCGA                 T^CGA
Tsp45I        161  GTCAC                ^GT[GC]AC
TthHB8I        68  TCGA                 T^CGA
XbaI          213  TCTAGA               T^CTAGA
XhoII         848  AGATCT               [GA]^GATC[CT]
XmnI          571  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

DraI          607  TTTAAA               TTT^AAA
              828  TTTAAA              
MaeII         604  ACGT                 A^CGT
              879  ACGT                
NlaIII        271  CATG                 CATG^
              885  CATG                
PleI          402  GACTC                GAGTC(4/5)
              904  GAGTC               
TfiI          388  GAATC                G^A[AT]TC
              442  GAATC               
TspRI         237  CACTG                CA[GC]TG[ACGT][ACGT]^
              345  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       BbvI        
BfaI        BsrI        BstF5I      DdeI        DpnII       
Fnu4HI      FokI        HinfI       ItaI        MaeI        
MboI        MboII       MnlI        MseI        MwoI        
NdeII       RsaI        Sau3AI      SfaNI       Tru9I       
TseI        Tsp509I