Restriction map for
pF1KB5748
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AocI Aor51HI ApaI AscI Asp700I
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BclI BfrI BglI
BglII BlnI BpuAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrFI
BsrGI BstBI BstXI Bsu36I Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HpaI KasI KspI MluI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SmlI
SpeI SphI Sse8387I SspBI StuI
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 150 TCCGGA T^CCGGA
AcsI 1435 AAATTC [GA]^AATT[CT]
AcyI 1675 GACGCC G[GA]^CG[CT]C
AhdI 490 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1862 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1435 AAATTC [GA]^AATT[CT]
AseI 1114 ATTAAT AT^TAAT
AsnI 1114 ATTAAT AT^TAAT
AspEI 490 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2060 CTCGGG C^[CT]CG[GA]G
BanII 417 GGGCTC G[GA]GC[CT]^C
BcgI 2101 CGACAGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 1820 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1675 GACGCC G[GA]^CG[CT]C
BsaWI 150 TCCGGA [AT]^CCGG[AT]
BseAI 150 TCCGGA T^CCGGA
BsmI 840 GAATGC GAATGC(1/-1)
BsoBI 2060 CTCGGG C^[CT]CG[GA]G
BspEI 150 TCCGGA T^CCGGA
BspHI 1967 TCATGA T^CATGA
BsrBI 1810 GAGCGG CCGCTC(-3/-3)
BsrDI 1082 GCAATG GCAATG(2/0)
BssHII 1780 GCGCGC G^CGCGC
Bst1107I 352 GTATAC GTA^TAC
BstAPI 284 GCAAGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1458 GGTGACC G^GT[ACGT]ACC
BstPI 1458 GGTGACC G^GT[ACGT]ACC
BstYI 523 AGATCC [GA]^GATC[CT]
BstZ17I 352 GTATAC GTA^TAC
DraII 1027 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1737 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1850 CCATGG C^C[GA][CT]GG
Eam1105I 490 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 804 GAAGAG CTCTTC(1/4)
EcoNI 1689 CCTTGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1027 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1458 GGTGACC G^GT[ACGT]ACC
Hin1I 1675 GACGCC G[GA]^CG[CT]C
HincII 1857 GTTGAC GT[CT]^[GA]AC
HindII 1857 GTTGAC GT[CT]^[GA]AC
HindIII 1495 AAGCTT A^AGCTT
Ksp632I 804 GAAGAG CTCTTC(1/4)
MamI 1820 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 523 AGATCC [GA]^GATC[CT]
MroI 150 TCCGGA T^CCGGA
NcoI 1850 CCATGG C^CATGG
PpuMI 1027 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1114 ATTAAT AT^TAAT
RcaI 1967 TCATGA T^CATGA
SexAI 2192 ACCAGGT A^CC[AT]GGT
SnaBI 349 TACGTA TAC^GTA
XcmI 992 CCACTTTCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 523 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 809 GGTACC G^GTACC
1772 GGTACC
AccI 346 GTCTAC GT^[AC][GT]AC
352 GTATAC
AccII 1779 CGCG CG^CG
1781 CGCG
Alw44I 232 GTGCAC G^TGCAC
511 GTGCAC
ApaLI 232 GTGCAC G^TGCAC
511 GTGCAC
Asp718I 809 GGTACC G^GTACC
1772 GGTACC
AspI 2173 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2191 GACCAGGTC
BalI 647 TGGCCA TGG^CCA
1946 TGGCCA
BanI 809 GGTACC G^G[CT][GA]CC
1772 GGTACC
BciVI 474 GGATAC GTATCC(6/5)
1819 GGATAC
BcnI 174 CCCGG CC^[GC]GG
245 CCCGG
BlpI 1077 GCTCAGC GC^T[ACGT]AGC
1683 GCTCAGC
Bpu1102I 1077 GCTCAGC GC^T[ACGT]AGC
1683 GCTCAGC
BsaAI 349 TACGTA [CT]AC^GT[GA]
1316 CACGTA
BsgI 982 CTGCAC GTGCAG(16/14)
532 GTGCAG
BspMI 889 ACCTGC ACCTGC(4/8)
1910 ACCTGC
BssSI 1010 CTCGTG CACGAG(-5/-1)
1501 CTCGTG
BstUI 1779 CGCG CG^CG
1781 CGCG
CelII 1077 GCTCAGC GC^T[ACGT]AGC
1683 GCTCAGC
DraI 1419 TTTAAA TTT^AAA
2185 TTTAAA
EcoT14I 199 CCTTGG C^C[AT][AT]GG
1850 CCATGG
FauI 167 CCCGC CCCGC(4/6)
1777 CCCGC
KpnI 809 GGTACC GGTAC^C
1772 GGTACC
MluNI 647 TGGCCA TGG^CCA
1946 TGGCCA
MscI 647 TGGCCA TGG^CCA
1946 TGGCCA
MvnI 1779 CGCG CG^CG
1781 CGCG
NciI 174 CCCGG CC^[GC]GG
245 CCCGG
NspI 785 ACATGC [GA]CATG^[CT]
2141 ACATGC
PflFI 2173 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2191 GACCAGGTC
PstI 1442 CTGCAG CTGCA^G
1508 CTGCAG
PvuII 35 CAGCTG CAG^CTG
2127 CAGCTG
ScaI 664 AGTACT AGT^ACT
1523 AGTACT
SfcI 1442 CTGCAG C^T[GA][CT]AG
1508 CTGCAG
SspI 1299 AATATT AAT^ATT
1883 AATATT
StyI 199 CCTTGG C^C[AT][AT]GG
1850 CCATGG
Tth111I 2173 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2191 GACCAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BfaI BmyI BpmI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MfeI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I