Restriction map for
pF1KE0787
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BseAI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstZ17I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 68 CGCG CG^CG
AocI 576 CCTCAGG CC^T[ACGT]AGG
Asp700I 425 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 263 CTCGAG C^[CT]CG[GA]G
AvaII 330 GGTCC G^G[AT]CC
BalI 761 TGGCCA TGG^CCA
BbsI 222 GAAGAC GAAGAC(2/6)
BlpI 1018 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1018 GCTGAGC GC^T[ACGT]AGC
BpuAI 222 GAAGAC GAAGAC(2/6)
BsaWI 421 ACCGGA [AT]^CCGG[AT]
BsiEI 202 CGACCG CG[GA][CT]^CG
BsmFI 771 GGGAC GGGAC(10/14)
BsoBI 263 CTCGAG C^[CT]CG[GA]G
Bsp1407I 659 TGTACA T^GTACA
BspMI 175 ACCTGC ACCTGC(4/8)
BsrGI 659 TGTACA T^GTACA
BssSI 898 CTCGTG CACGAG(-5/-1)
BstEII 540 GGTGACC G^GT[ACGT]ACC
BstPI 540 GGTGACC G^GT[ACGT]ACC
BstUI 68 CGCG CG^CG
Bsu36I 576 CCTCAGG CC^T[ACGT]AGG
CelII 1018 GCTGAGC GC^T[ACGT]AGC
CfoI 67 GCGC GCG^C
DsaI 236 CCACGG C^C[GA][CT]GG
Eco81I 576 CCTCAGG CC^T[ACGT]AGG
EcoO65I 540 GGTGACC G^GT[ACGT]ACC
HgaI 798 GCGTC GACGC(5/10)
HhaI 67 GCGC GCG^C
HinP1I 67 GCGC G^CGC
HindIII 109 AAGCTT A^AGCTT
MluNI 761 TGGCCA TGG^CCA
MscI 761 TGGCCA TGG^CCA
MspA1I 337 CAGCTG C[AC]G^C[GT]G
MvnI 68 CGCG CG^CG
PaeR7I 263 CTCGAG C^TCGAG
PflMI 868 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 337 CAGCTG CAG^CTG
ScaI 242 AGTACT AGT^ACT
SspBI 659 TGTACA T^GTACA
TaqI 264 TCGA T^CGA
TfiI 425 GAATC G^A[AT]TC
Tsp509I 708 AATT ^AATT
TthHB8I 264 TCGA T^CGA
Van91I 868 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 263 CTCGAG C^TCGAG
XmnI 425 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 334 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
634 CAGTTCCTG
AspHI 13 GTGCTC G[AT]GC[AT]^C
717 GAGCAC
BamHI 149 GGATCC G^GATCC
819 GGATCC
BcnI 299 CCGGG CC^[GC]GG
322 CCGGG
BmyI 13 GTGCTC G[AGT]GC[ACT]^C
717 GAGCAC
BsgI 165 CTGCAC GTGCAG(16/14)
340 CTGCAC
BsiHKAI 13 GTGCTC G[AT]GC[AT]^C
717 GAGCAC
Bsp1286I 13 GTGCTC G[AGT]GC[ACT]^C
717 GAGCAC
BsrBI 205 CCGCTC CCGCTC(-3/-3)
271 GAGCGG
BstXI 524 CCAGCCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
554 CCATGCTCATGG
EcoT14I 140 CCAAGG C^C[AT][AT]GG
698 CCAAGG
FauI 372 CCCGC CCCGC(4/6)
273 GCGGG
MaeIII 130 GTGAC ^GT[ACGT]AC
541 GTGAC
NciI 299 CCGGG CC^[GC]GG
322 CCGGG
PleI 225 GACTC GAGTC(4/5)
646 GACTC
StuI 254 AGGCCT AGG^CCT
580 AGGCCT
StyI 140 CCAAGG C^C[AT][AT]GG
698 CCAAGG
Tsp45I 130 GTGAC ^GT[GC]AC
541 GTGAC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BpmI BsaJI BseRI BsiYI BslI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI Tru9I
TseI TspRI XhoII