Restriction map for
pF1KB6863
##################################
# NO CUT ENZYMES ( 176 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BcgI BciVI BclI
BcnI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmFI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FauI FbaI Fnu4HI FseI
FspI HaeII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
ItaI KasI KpnI KspI MamI
MfeI MflI MluI MroI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
TaqI TseI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AcsI 5 GAATTC [GA]^AATT[CT]
AflII 526 CTTAAG C^TTAAG
AflIII 178 ACACGT A^C[GA][CT]GT
ApoI 5 GAATTC [GA]^AATT[CT]
AseI 169 ATTAAT AT^TAAT
AsnI 169 ATTAAT AT^TAAT
Asp700I 211 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 339 GGACC G^G[AT]CC
BalI 204 TGGCCA TGG^CCA
BfrI 526 CTTAAG C^TTAAG
BsaJI 498 CCAAGG C^C[ACGT][ACGT]GG
BsmAI 27 GAGAC GTCTC(1/5)
BsmI 391 GAATGC GAATGC(1/-1)
Bsp1407I 123 TGTACA T^GTACA
BsrGI 123 TGTACA T^GTACA
BstAPI 466 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 512 GGATG GGATG(2/0)
Cac8I 466 GCATGC GC[ACGT]^[ACGT]GC
CfoI 331 GCGC GCG^C
Cfr13I 339 GGACC G^G[ACGT]CC
DdeI 343 CTAAG C^T[ACGT]AG
DpnII 445 GATC ^GATC
DraII 338 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 204 TGGCCA [CT]^GGCC[GA]
EcoNI 136 CCTACAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 338 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 5 GAATTC G^AATTC
EcoT14I 498 CCAAGG C^C[AT][AT]GG
EcoT22I 393 ATGCAT ATGCA^T
FokI 512 GGATG GGATG(9/13)
HaeIII 205 GGCC GG^CC
HhaI 331 GCGC GCG^C
HinP1I 331 GCGC G^CGC
HinfI 211 GAATC G^A[ACGT]TC
MaeII 180 ACGT A^CGT
MboI 445 GATC ^GATC
MluNI 204 TGGCCA TGG^CCA
MscI 204 TGGCCA TGG^CCA
MwoI 466 GCATGCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 445 GATC ^GATC
NsiI 393 ATGCAT ATGCA^T
NspI 466 GCATGC [GA]CATG^[CT]
PpuMI 338 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 169 ATTAAT AT^TAAT
Sau3AI 445 GATC ^GATC
Sau96I 339 GGACC G^G[ACGT]CC
SfcI 401 CTGTAG C^T[GA][CT]AG
SmlI 526 CTTAAG C^T[CT][GA]AG
SphI 466 GCATGC GCATG^C
SspBI 123 TGTACA T^GTACA
StyI 498 CCAAGG C^C[AT][AT]GG
TfiI 211 GAATC G^A[AT]TC
Tsp45I 519 GTCAC ^GT[GC]AC
TspRI 451 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 211 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 124 GTAC GT^AC
278 GTAC
AluI 351 AGCT AG^CT
428 AGCT
AspHI 46 GAGCAC G[AT]GC[AT]^C
473 GTGCTC
BfaI 17 CTAG C^TAG
305 CTAG
BmyI 46 GAGCAC G[AGT]GC[ACT]^C
473 GTGCTC
BsiHKAI 46 GAGCAC G[AT]GC[AT]^C
473 GTGCTC
BsiYI 136 CCTACAATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
186 CCTAATGTGGG
BslI 136 CCTACAATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
186 CCTAATGTGGG
Bsp1286I 46 GAGCAC G[AGT]GC[ACT]^C
473 GTGCTC
HphI 39 TCACC GGTGA(8/7)
447 TCACC
MaeI 17 CTAG C^TAG
305 CTAG
MaeIII 301 GTAAC ^GT[ACGT]AC
519 GTCAC
RsaI 124 GTAC GT^AC
278 GTAC
Tsp509I 6 AATT ^AATT
104 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
BsrI BssKI BstNI EarI EcoRII
Ksp632I MboII MnlI MseI MvaI
NlaIII ScrFI Tru9I