Restriction map for
pF1KB5572
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiWI BsmBI BsoBI BspDI
BspEI BsrDI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HindIII HpaI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 117 CGCG CG^CG
AcsI 1132 AAATTT [GA]^AATT[CT]
AflIII 364 ACATGT A^C[GA][CT]GT
Alw44I 360 GTGCAC G^TGCAC
AlwNI 291 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 360 GTGCAC G^TGCAC
ApoI 1132 AAATTT [GA]^AATT[CT]
BglI 530 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 475 GAGGAG GAGGAG(10/8)
BsgI 966 CTGCAC GTGCAG(16/14)
BsiEI 957 CGGCCG CG[GA][CT]^CG
BsmAI 1021 GAGAC GTCTC(1/5)
Bsp1407I 827 TGTACA T^GTACA
BspHI 1040 TCATGA T^CATGA
BspLU11I 364 ACATGT A^CATGT
BspMI 73 ACCTGC ACCTGC(4/8)
BsrBI 254 CCGCTC CCGCTC(-3/-3)
BsrFI 926 ACCGGC [GA]^CCGG[CT]
BsrGI 827 TGTACA T^GTACA
BssHII 116 GCGCGC G^CGCGC
BssSI 746 CACGAG CACGAG(-5/-1)
BstAPI 848 GCAAGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 117 CGCG CG^CG
BstXI 218 CCAAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 266 GGATCT [GA]^GATC[CT]
Cfr10I 926 ACCGGC [GA]^CCGG[CT]
DraII 506 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 957 CGGCCG [CT]^GGCC[GA]
EagI 957 CGGCCG C^GGCCG
EclXI 957 CGGCCG C^GGCCG
Eco52I 957 CGGCCG C^GGCCG
EcoO109I 506 AGGACCC [GA]G^G[ACGT]CC[CT]
HincII 573 GTTGAC GT[CT]^[GA]AC
HindII 573 GTTGAC GT[CT]^[GA]AC
HphI 38 TCACC GGTGA(8/7)
MaeII 1037 ACGT A^CGT
MflI 266 GGATCT [GA]^GATC[CT]
MslI 1081 CATGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 117 CGCG CG^CG
NcoI 512 CCATGG C^CATGG
PleI 125 GAGTC GAGTC(4/5)
PpuMI 506 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 976 CAGCTG CAG^CTG
RcaI 1040 TCATGA T^CATGA
ScaI 935 AGTACT AGT^ACT
SphI 1080 GCATGC GCATG^C
SspBI 827 TGTACA T^GTACA
TaqI 554 TCGA T^CGA
TthHB8I 554 TCGA T^CGA
XcmI 437 CCAACCCTCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 266 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 828 GTAC GT^AC
936 GTAC
AlwI 1047 GATCC GGATC(4/5)
266 GGATC
BanII 3 GAGCTC G[GA]GC[CT]^C
275 GAGCTC
BpmI 309 CTCCAG CTGGAG(16/14)
100 CTGGAG
BsmI 229 GAATGC GAATGC(1/-1)
389 GCATTC
BstF5I 654 CATCC GGATG(2/0)
904 GGATG
DpnII 267 GATC ^GATC
1047 GATC
DsaI 380 CCGTGG C^C[GA][CT]GG
512 CCATGG
FokI 654 CATCC GGATG(9/13)
904 GGATG
MboI 267 GATC ^GATC
1047 GATC
NdeII 267 GATC ^GATC
1047 GATC
NspI 364 ACATGT [GA]CATG^[CT]
1080 GCATGC
PstI 189 CTGCAG CTGCA^G
270 CTGCAG
RsaI 828 GTAC GT^AC
936 GTAC
SacI 3 GAGCTC GAGCT^C
275 GAGCTC
Sau3AI 267 GATC ^GATC
1047 GATC
TfiI 314 GAATC G^A[AT]TC
551 GATTC
Tsp45I 37 GTCAC ^GT[GC]AC
488 GTGAC
Tsp509I 590 AATT ^AATT
1133 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcyI AluI AspHI AvaII
BanI BbeI BbvI BcnI BmyI
BsaHI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI EcoNI
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII ItaI KasI MaeIII
MboII MnlI MspA1I MspI MvaI
MwoI NarI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI SfcI SfoI
StyI TseI TspRI