################################## # NO CUT ENZYMES ( 199 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MamI MboI MflI MluI MluNI MroI MscI MslI MspA1I MspI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 220 GACGTC GACGT^C AciI 231 CCGC CCGC(-3/-1) AcyI 220 GACGTC G[GA]^CG[CT]C AfaI 255 GTAC GT^AC AvaII 273 GGACC G^G[AT]CC BbsI 52 GTCTTC GAAGAC(2/6) BpmI 369 CTGGAG CTGGAG(16/14) BpuAI 52 GTCTTC GAAGAC(2/6) BsaHI 220 GACGTC G[GA]^CG[CT]C BsaJI 9 CCAAGG C^C[ACGT][ACGT]GG BsmAI 152 GTCTC GTCTC(1/5) BsmFI 310 GGGAC GGGAC(10/14) BsrDI 205 CATTGC GCAATG(2/0) Cac8I 74 GCCTGC GC[ACGT]^[ACGT]GC Cfr13I 273 GGACC G^G[ACGT]CC DraII 272 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO109I 272 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 9 CCAAGG C^C[AT][AT]GG FauI 230 CCCGC CCCGC(4/6) Hin1I 220 GACGTC G[GA]^CG[CT]C HphI 345 GGTGA GGTGA(8/7) MfeI 126 CAATTG C^AATTG MseI 287 TTAA T^TAA MunI 126 CAATTG C^AATTG MwoI 74 GCCTGCAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 371 GGAGCC GG[ACGT]^[ACGT]CC PleI 348 GACTC GAGTC(4/5) PpuMI 272 AGGACCT [GA]G^G[AT]CC[CT] PstI 76 CTGCAG CTGCA^G RsaI 255 GTAC GT^AC Sau96I 273 GGACC G^G[ACGT]CC SfaNI 145 GATGC GCATC(5/9) SfcI 76 CTGCAG C^T[GA][CT]AG SmlI 234 CTTGAG C^T[CT][GA]AG StyI 9 CCAAGG C^C[AT][AT]GG TfiI 5 GAATC G^A[AT]TC Tru9I 287 TTAA T^TAA TspRI 79 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BssKI 45 CCTGG ^CC[ACGT]GG 368 CCTGG BstF5I 314 CATCC GGATG(2/0) 379 CATCC BstNI 45 CCTGG CC^[AT]GG 368 CCTGG EcoRII 45 CCTGG ^CC[AT]GG 368 CCTGG FokI 314 CATCC GGATG(9/13) 379 CATCC HinfI 5 GAATC G^A[ACGT]TC 348 GACTC MaeII 221 ACGT A^CGT 253 ACGT MboII 53 TCTTC GAAGA(8/7) 336 TCTTC MnlI 14 GAGG CCTC(7/6) 237 GAGG MvaI 45 CCTGG CC^[AT]GG 368 CCTGG ScrFI 45 CCTGG CC^[ACGT]GG 368 CCTGG Tsp45I 223 GTCAC ^GT[GC]AC 346 GTGAC Tsp509I 127 AATT ^AATT 211 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AluI BbvI BsiYI BslI Fnu4HI ItaI MaeIII TseI