Restriction map for
pF1KB5749
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# NO CUT ENZYMES ( 95 enzymes) #
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AccIII AclI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AvrII BbeI
BbrPI BcgI BfrI BglI BlnI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoRI EcoT22I FseI HgaI HincII
HindII HindIII HpaI KasI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI SspBI StuI SwaI
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 652 GACGTC GACGT^C
Acc65I 7 GGTACC G^GTACC
AccII 338 CGCG CG^CG
AcyI 652 GACGTC G[GA]^CG[CT]C
AhdI 1234 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1057 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 2232 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 7 GGTACC G^GTACC
AspEI 1234 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1088 CCCGAG C^[CT]CG[GA]G
BamHI 1467 GGATCC G^GATCC
BanI 7 GGTACC G^G[CT][GA]CC
BciVI 917 GTATCC GTATCC(6/5)
BclI 116 TGATCA T^GATCA
BglII 1487 AGATCT A^GATCT
BlpI 814 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 814 GCTCAGC GC^T[ACGT]AGC
BsaAI 295 TACGTA [CT]AC^GT[GA]
BsaBI 2380 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 652 GACGTC G[GA]^CG[CT]C
BsmBI 330 CGTCTC CGTCTC(1/5)
BsoBI 1088 CCCGAG C^[CT]CG[GA]G
BspHI 1700 TCATGA T^CATGA
BsrBI 642 CCGCTC CCGCTC(-3/-3)
BstEII 694 GGTGACC G^GT[ACGT]ACC
BstPI 694 GGTGACC G^GT[ACGT]ACC
BstUI 338 CGCG CG^CG
CelII 814 GCTCAGC GC^T[ACGT]AGC
Eam1105I 1234 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1743 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 694 GGTGACC G^GT[ACGT]ACC
FbaI 116 TGATCA T^GATCA
HaeII 1169 AGCGCC [GA]GCGC^[CT]
Hin1I 652 GACGTC G[GA]^CG[CT]C
KpnI 7 GGTACC GGTAC^C
KspI 337 CCGCGG CCGC^GG
MamI 2380 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 338 CGCG CG^CG
NaeI 628 GCCGGC GCC^GGC
NgoMIV 628 GCCGGC G^CCGGC
PflMI 2202 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 760 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 1700 TCATGA T^CATGA
SacI 1809 GAGCTC GAGCT^C
SacII 337 CCGCGG CCGC^GG
SbfI 1914 CCTGCAGG CCTGCA^GG
SnaBI 295 TACGTA TAC^GTA
Sse8387I 1914 CCTGCAGG CCTGCA^GG
SspI 1018 AATATT AAT^ATT
Van91I 2202 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2232 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 574 GTCTAC GT^[AC][GT]AC
1363 GTCTAC
AcsI 89 GAATTT [GA]^AATT[CT]
1228 GAATTT
ApoI 89 GAATTT [GA]^AATT[CT]
1228 GAATTT
AspHI 1523 GAGCAC G[AT]GC[AT]^C
1809 GAGCTC
AspI 931 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1495 GACCATGTC
BbvCI 360 CCTCAGC CCTCAGC(-5/-2)
832 GCTGAGG
BfaI 406 CTAG C^TAG
985 CTAG
BsgI 1082 CTGCAC GTGCAG(16/14)
1691 GTGCAG
BsiHKAI 1523 GAGCAC G[AT]GC[AT]^C
1809 GAGCTC
BsrDI 1296 CATTGC GCAATG(2/0)
1767 CATTGC
DraII 620 AGGCCCC [GA]G^G[ACGT]CC[CT]
760 GGGACCT
DrdI 1746 GACACAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2383 GACAATCAGGTC
EcoO109I 620 AGGCCCC [GA]G^G[ACGT]CC[CT]
760 GGGACCT
EcoRV 880 GATATC GAT^ATC
1573 GATATC
MaeI 406 CTAG C^TAG
985 CTAG
NcoI 921 CCATGG C^CATGG
1190 CCATGG
PflFI 931 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1495 GACCATGTC
PstI 1335 CTGCAG CTGCA^G
1915 CTGCAG
SapI 2329 GAAGAGC GCTCTTC(1/4)
1443 GCTCTTC
SmlI 550 CTTGAG C^T[CT][GA]AG
2153 CTCAAG
TaqI 1287 TCGA T^CGA
1866 TCGA
Tsp509I 90 AATT ^AATT
1229 AATT
Tth111I 931 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1495 GACCATGTC
TthHB8I 1287 TCGA T^CGA
1866 TCGA
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
AviII BalI BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I TspRI XhoII