Restriction map for
pF1KE1303
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BfrI BglI BlnI BsaAI BsaBI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NarI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 748 GTCTAC GT^[AC][GT]AC
AccII 94 CGCG CG^CG
AccIII 1220 TCCGGA T^CCGGA
AfaI 1086 GTAC GT^AC
AhdI 1135 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 792 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1135 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 480 TGGCCA TGG^CCA
BanI 32 GGTGCC G^G[CT][GA]CC
BclI 173 TGATCA T^GATCA
BcnI 524 CCGGG CC^[GC]GG
BglII 180 AGATCT A^GATCT
BlpI 87 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 87 GCTGAGC GC^T[ACGT]AGC
BsaI 1093 GAGACC GGTCTC(1/5)
BseAI 1220 TCCGGA T^CCGGA
BseRI 582 CTCCTC GAGGAG(10/8)
BsiEI 776 CGGTCG CG[GA][CT]^CG
BspEI 1220 TCCGGA T^CCGGA
BsrFI 35 GCCGGC [GA]^CCGG[CT]
BstUI 94 CGCG CG^CG
CelII 87 GCTGAGC GC^T[ACGT]AGC
CfoI 658 GCGC GCG^C
Cfr10I 35 GCCGGC [GA]^CCGG[CT]
DraI 1237 TTTAAA TTT^AAA
DraIII 1010 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 480 TGGCCA [CT]^GGCC[GA]
Eam1105I 1135 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 879 CCATGG C^C[AT][AT]GG
FbaI 173 TGATCA T^GATCA
HhaI 658 GCGC GCG^C
HinP1I 658 GCGC G^CGC
MaeII 1088 ACGT A^CGT
MluNI 480 TGGCCA TGG^CCA
MroI 1220 TCCGGA T^CCGGA
MscI 480 TGGCCA TGG^CCA
MslI 1020 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 94 CGCG CG^CG
NaeI 35 GCCGGC GCC^GGC
NciI 524 CCGGG CC^[GC]GG
NcoI 879 CCATGG C^CATGG
NdeI 112 CATATG CA^TATG
NgoMIV 35 GCCGGC G^CCGGC
NspI 248 ACATGC [GA]CATG^[CT]
PaeR7I 191 CTCGAG C^TCGAG
RsaI 1086 GTAC GT^AC
StuI 854 AGGCCT AGG^CCT
StyI 879 CCATGG C^C[AT][AT]GG
TfiI 342 GATTC G^A[AT]TC
Tsp45I 1016 GTGAC ^GT[GC]AC
Tsp509I 1213 AATT ^AATT
XcmI 852 CCAGGCCTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 191 CTCGAG C^TCGAG
XmnI 792 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 137 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
740 CAGGGGCTG
AvaI 191 CTCGAG C^[CT]CG[GA]G
934 CCCGAG
BbsI 910 GAAGAC GAAGAC(2/6)
1132 GAAGAC
BfaI 307 CTAG C^TAG
998 CTAG
BpuAI 910 GAAGAC GAAGAC(2/6)
1132 GAAGAC
BsaWI 366 ACCGGA [AT]^CCGG[AT]
1220 TCCGGA
BsmAI 187 GAGAC GTCTC(1/5)
1093 GAGAC
BsoBI 191 CTCGAG C^[CT]CG[GA]G
934 CCCGAG
BstYI 145 GGATCT [GA]^GATC[CT]
180 AGATCT
DsaI 669 CCACGG C^C[GA][CT]GG
879 CCATGG
EarI 206 CTCTTC CTCTTC(1/4)
52 GAAGAG
Ksp632I 206 CTCTTC CTCTTC(1/4)
52 GAAGAG
MaeI 307 CTAG C^TAG
998 CTAG
MaeIII 929 GTAAC ^GT[ACGT]AC
1016 GTGAC
MflI 145 GGATCT [GA]^GATC[CT]
180 AGATCT
MseI 1211 TTAA T^TAA
1238 TTAA
PpuMI 970 AGGACCT [GA]G^G[AT]CC[CT]
1064 GGGACCC
SapI 52 GAAGAGC GCTCTTC(1/4)
205 GCTCTTC
SmlI 191 CTCGAG C^T[CT][GA]AG
424 CTTGAG
Tru9I 1211 TTAA T^TAA
1238 TTAA
XhoII 145 GGATCT [GA]^GATC[CT]
180 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcyI AluI AlwI AspHI
AvaII BanII BbvI BcgI BmyI
BpmI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI Hin1I HinfI HpaII
HphI ItaI MboI MboII MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI TspRI TthHB8I