Restriction map for
pF1KE1415
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# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AvrII
BalI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmFI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HaeII HapII HgaI Hin1I
HindIII HpaI HpaII HphI KasI
KpnI Ksp632I KspI MaeIII MamI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp45I Tsp509I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 170 GTCGAC GT^[AC][GT]AC
AfaI 385 GTAC GT^AC
AlwNI 22 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 324 GTGCTC G[AT]GC[AT]^C
AvaI 145 CCCGAG C^[CT]CG[GA]G
AviII 505 TGCGCA TGC^GCA
BamHI 347 GGATCC G^GATCC
BanI 276 GGCACC G^G[CT][GA]CC
BfaI 37 CTAG C^TAG
BpmI 177 CTGGAG CTGGAG(16/14)
BseRI 667 GAGGAG GAGGAG(10/8)
BsiHKAI 324 GTGCTC G[AT]GC[AT]^C
BsmI 186 GAATGC GAATGC(1/-1)
BsoBI 145 CCCGAG C^[CT]CG[GA]G
BsrBI 379 GAGCGG CCGCTC(-3/-3)
BstYI 347 GGATCC [GA]^GATC[CT]
EaeI 618 TGGCCG [CT]^GGCC[GA]
FauI 381 GCGGG CCCGC(4/6)
FspI 505 TGCGCA TGC^GCA
HincII 170 GTCGAC GT[CT]^[GA]AC
HindII 170 GTCGAC GT[CT]^[GA]AC
MaeI 37 CTAG C^TAG
MaeII 392 ACGT A^CGT
MflI 347 GGATCC [GA]^GATC[CT]
MseI 497 TTAA T^TAA
PflMI 351 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 579 GACTC GAGTC(4/5)
PstI 689 CTGCAG CTGCA^G
PvuII 22 CAGCTG CAG^CTG
RsaI 385 GTAC GT^AC
SalI 170 GTCGAC G^TCGAC
SfaNI 626 GCATC GCATC(5/9)
TfiI 248 GATTC G^A[AT]TC
Tru9I 497 TTAA T^TAA
Van91I 351 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 662 CCAATGAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 347 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
ApaI 340 GGGCCC GGGCC^C
709 GGGCCC
BanII 340 GGGCCC G[GA]GC[CT]^C
709 GGGCCC
BsrDI 420 GCAATG GCAATG(2/0)
566 GCAATG
CfoI 372 GCGC GCG^C
506 GCGC
DpnII 348 GATC ^GATC
361 GATC
HhaI 372 GCGC GCG^C
506 GCGC
HinP1I 372 GCGC G^CGC
506 GCGC
HinfI 248 GATTC G^A[ACGT]TC
579 GACTC
MboI 348 GATC ^GATC
361 GATC
MboII 183 GAAGA GAAGA(8/7)
430 GAAGA
MslI 223 CATAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
627 CATCCAGATG
NdeII 348 GATC ^GATC
361 GATC
Sau3AI 348 GATC ^GATC
361 GATC
TaqI 171 TCGA T^CGA
641 TCGA
TthHB8I 171 TCGA T^CGA
641 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AlwI BbvI BlpI
BmyI Bpu1102I BsaJI BsiYI BslI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CelII Cfr13I DdeI
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII ItaI MnlI MspA1I MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfcI TseI TspRI