Restriction map for
pF1KE2649
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiHKAI BsiWI
BsiYI BslI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StyI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 79 AAATTT [GA]^AATT[CT]
AfaI 97 GTAC GT^AC
AflIII 49 ACATGT A^C[GA][CT]GT
AluI 431 AGCT AG^CT
AlwNI 416 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 79 AAATTT [GA]^AATT[CT]
BanI 291 GGTGCC G^G[CT][GA]CC
BfaI 235 CTAG C^TAG
BsaI 252 GGTCTC GGTCTC(1/5)
BsaJI 527 CCCTGG C^C[ACGT][ACGT]GG
BsgI 473 CTGCAC GTGCAG(16/14)
BsiEI 202 CGACCG CG[GA][CT]^CG
BsmFI 340 GGGAC GGGAC(10/14)
BspLU11I 49 ACATGT A^CATGT
BspMI 557 GCAGGT ACCTGC(4/8)
BssKI 528 CCTGG ^CC[ACGT]GG
BstNI 528 CCTGG CC^[AT]GG
Cac8I 432 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 435 GGCCC G^G[ACGT]CC
DpnII 352 GATC ^GATC
EarI 549 GAAGAG CTCTTC(1/4)
EcoRII 528 CCTGG ^CC[AT]GG
EcoRV 60 GATATC GAT^ATC
HphI 306 TCACC GGTGA(8/7)
Ksp632I 549 GAAGAG CTCTTC(1/4)
MaeI 235 CTAG C^TAG
MaeII 275 ACGT A^CGT
MboI 352 GATC ^GATC
MseI 11 TTAA T^TAA
MslI 126 CACAGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 205 CCGCTG C[AC]G^C[GT]G
MvaI 528 CCTGG CC^[AT]GG
MwoI 514 GCAATATTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 352 GATC ^GATC
NlaIII 50 CATG CATG^
NspI 49 ACATGT [GA]CATG^[CT]
PstI 173 CTGCAG CTGCA^G
RsaI 97 GTAC GT^AC
SapI 549 GAAGAGC GCTCTTC(1/4)
Sau3AI 352 GATC ^GATC
Sau96I 435 GGCCC G^G[ACGT]CC
ScrFI 528 CCTGG CC^[ACGT]GG
SfcI 173 CTGCAG C^T[GA][CT]AG
SspI 516 AATATT AAT^ATT
StuI 489 AGGCCT AGG^CCT
Tru9I 11 TTAA T^TAA
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AciI 205 CCGC CCGC(-3/-1)
555 CCGC
BbvI 523 GCTGC GCAGC(8/12)
567 GCTGC
HaeIII 435 GGCC GG^CC
490 GGCC
HinfI 321 GACTC G^A[ACGT]TC
342 GACTC
MaeIII 124 GTCAC ^GT[ACGT]AC
440 GTGAC
NlaIV 291 GGTGCC GG[ACGT]^[ACGT]CC
531 GGAGCC
PleI 321 GACTC GAGTC(4/5)
342 GACTC
TseI 523 GCTGC G^C[AT]GC
567 GCTGC
Tsp45I 124 GTCAC ^GT[GC]AC
440 GTGAC
TspRI 248 CAGTG CA[GC]TG[ACGT][ACGT]^
310 CACTG
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
BpmI BsmAI DdeI Fnu4HI ItaI
MboII MnlI Tsp509I