Restriction map for
pF1KE1305
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflIII AgeI Alw44I Aor51HI ApaLI
AscI Asp718I AviII BamHI BbeI
BbrPI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII Bst1107I BstBI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DsaI
Eco47III EcoRI EcoRV FseI FspI
Hin1I HpaI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NcoI NgoMIV NheI NotI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SpeI Sse8387I SwaI
Van91I XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 3420 GTCTAC GT^[AC][GT]AC
AhdI 3136 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1195 CCTGAGG CC^T[ACGT]AGG
AseI 2362 ATTAAT AT^TAAT
AsnI 2362 ATTAAT AT^TAAT
AspEI 3136 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2143 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 112 CCCGGG C^[CT]CG[GA]G
AvrII 3789 CCTAGG C^CTAGG
BalI 1650 TGGCCA TGG^CCA
BanI 3532 GGTGCC G^G[CT][GA]CC
BbvCI 959 CCTCAGC CCTCAGC(-5/-2)
BcgI 1167 CGATTTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2736 GGATAC GTATCC(6/5)
BlnI 3789 CCTAGG C^CTAGG
BsaAI 640 TACGTA [CT]AC^GT[GA]
BsaI 2486 GGTCTC GGTCTC(1/5)
BsiEI 93 CGGCCG CG[GA][CT]^CG
BsoBI 112 CCCGGG C^[CT]CG[GA]G
BspMI 2664 GCAGGT ACCTGC(4/8)
BstAPI 3907 GCAAGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 304 GGTTACC G^GT[ACGT]ACC
BstPI 304 GGTTACC G^GT[ACGT]ACC
Bsu36I 1195 CCTGAGG CC^T[ACGT]AGG
DraI 441 TTTAAA TTT^AAA
DrdI 1986 GACTTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 93 CGGCCG C^GGCCG
Eam1105I 3136 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 93 CGGCCG C^GGCCG
Eco52I 93 CGGCCG C^GGCCG
Eco81I 1195 CCTGAGG CC^T[ACGT]AGG
EcoO65I 304 GGTTACC G^GT[ACGT]ACC
FauI 134 GCGGG CCCGC(4/6)
HgaI 190 GCGTC GACGC(5/10)
HindIII 2440 AAGCTT A^AGCTT
MfeI 3506 CAATTG C^AATTG
MluNI 1650 TGGCCA TGG^CCA
MscI 1650 TGGCCA TGG^CCA
MunI 3506 CAATTG C^AATTG
NruI 4 TCGCGA TCG^CGA
PflFI 2143 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2362 ATTAAT AT^TAAT
PstI 2677 CTGCAG CTGCA^G
PvuII 2932 CAGCTG CAG^CTG
SmaI 112 CCCGGG CCC^GGG
SnaBI 640 TACGTA TAC^GTA
SspI 1922 AATATT AAT^ATT
Tth111I 2143 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2373 TCTAGA T^CTAGA
XmaCI 112 CCCGGG C^CCGGG
XmaI 112 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AflII 1117 CTTAAG C^TTAAG
2659 CTTAAG
AlwNI 755 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
1991 CAGAGTCTG
ApaI 166 GGGCCC GGGCC^C
1367 GGGCCC
Asp700I 2122 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3931 GAAGTTATTC
AspHI 12 GAGCAC G[AT]GC[AT]^C
1141 GAGCAC
BclI 1158 TGATCA T^GATCA
2088 TGATCA
BfrI 1117 CTTAAG C^TTAAG
2659 CTTAAG
BglI 58 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
109 GCCCCCGGGGC
BpmI 1463 CTCCAG CTGGAG(16/14)
147 CTGGAG
BsiHKAI 12 GAGCAC G[AT]GC[AT]^C
1141 GAGCAC
Bsp1407I 20 TGTACA T^GTACA
390 TGTACA
BspHI 1098 TCATGA T^CATGA
1352 TCATGA
BsrDI 523 GCAATG GCAATG(2/0)
2325 GCAATG
BsrGI 20 TGTACA T^GTACA
390 TGTACA
BssSI 1442 CACGAG CACGAG(-5/-1)
1658 CTCGTG
BstXI 170 CCAGGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1050 CCACGTTAGTGG
FbaI 1158 TGATCA T^GATCA
2088 TGATCA
HaeII 45 GGCGCT [GA]GCGC^[CT]
240 AGCGCC
HincII 2010 GTTGAC GT[CT]^[GA]AC
4026 GTCAAC
HindII 2010 GTTGAC GT[CT]^[GA]AC
4026 GTCAAC
MspA1I 104 CCGCTG C[AC]G^C[GT]G
2932 CAGCTG
NdeI 1664 CATATG CA^TATG
3796 CATATG
PshAI 1072 GACAGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2895 GACCATAGTC
RcaI 1098 TCATGA T^CATGA
1352 TCATGA
SspBI 20 TGTACA T^GTACA
390 TGTACA
XmnI 2122 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3931 GAAGTTATTC
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII