Restriction map for
pF1KB6986
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AviII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfrI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI MfeI MluI MroI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Van91I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
ApaI 257 GGGCCC GGGCC^C
Asp700I 589 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 297 GGACC G^G[AT]CC
AvrII 424 CCTAGG C^CTAGG
BalI 149 TGGCCA TGG^CCA
BanI 490 GGTGCC G^G[CT][GA]CC
BbsI 25 GTCTTC GAAGAC(2/6)
BbvI 345 GCAGC GCAGC(8/12)
BclI 572 TGATCA T^GATCA
BfaI 425 CTAG C^TAG
BglI 463 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 182 AGATCT A^GATCT
BlnI 424 CCTAGG C^CTAGG
BpmI 514 CTGGAG CTGGAG(16/14)
BpuAI 25 GTCTTC GAAGAC(2/6)
BsaBI 243 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 113 GAGAC GTCTC(1/5)
BspHI 629 TCATGA T^CATGA
BsrDI 362 GCAATG GCAATG(2/0)
BsrFI 281 ACCGGC [GA]^CCGG[CT]
BsrI 335 CCAGT ACTGG(1/-1)
BstXI 560 CCATTAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 182 AGATCT [GA]^GATC[CT]
Cfr10I 281 ACCGGC [GA]^CCGG[CT]
DraI 657 TTTAAA TTT^AAA
DsaI 92 CCGCGG C^C[GA][CT]GG
EcoT14I 424 CCTAGG C^C[AT][AT]GG
FauI 323 CCCGC CCCGC(4/6)
FbaI 572 TGATCA T^GATCA
Fnu4HI 345 GCAGC GC^[ACGT]GC
ItaI 345 GCAGC GC^[ACGT]GC
KspI 92 CCGCGG CCGC^GG
MaeI 425 CTAG C^TAG
MaeII 203 ACGT A^CGT
MaeIII 304 GTCAC ^GT[ACGT]AC
MamI 243 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 182 AGATCT [GA]^GATC[CT]
MluNI 149 TGGCCA TGG^CCA
MscI 149 TGGCCA TGG^CCA
MspA1I 92 CCGCGG C[AC]G^C[GT]G
PpuMI 296 AGGACCC [GA]G^G[AT]CC[CT]
PstI 157 CTGCAG CTGCA^G
RcaI 629 TCATGA T^CATGA
SacII 92 CCGCGG CCGC^GG
SfcI 157 CTGCAG C^T[GA][CT]AG
SmaI 260 CCCGGG CCC^GGG
SmlI 649 CTCAAG C^T[CT][GA]AG
StuI 222 AGGCCT AGG^CCT
StyI 424 CCTAGG C^C[AT][AT]GG
TseI 345 GCAGC G^C[AT]GC
Tsp45I 304 GTCAC ^GT[GC]AC
XhoII 182 AGATCT [GA]^GATC[CT]
XmaCI 260 CCCGGG C^CCGGG
XmaI 260 CCCGGG C^CCGGG
XmnI 589 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 60 GTAC GT^AC
315 GTAC
AluI 4 AGCT AG^CT
566 AGCT
AspI 199 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
298 GACCCGGTC
AvaI 260 CCCGGG C^[CT]CG[GA]G
447 CTCGGG
BanII 84 GGGCTC G[GA]GC[CT]^C
257 GGGCCC
BsiEI 9 CGACCG CG[GA][CT]^CG
89 CGACCG
BsoBI 260 CCCGGG C^[CT]CG[GA]G
447 CTCGGG
BstF5I 162 GGATG GGATG(2/0)
195 GGATG
Cac8I 220 GCAGGC GC[ACGT]^[ACGT]GC
371 GCAGGC
DraII 256 AGGGCCC [GA]G^G[ACGT]CC[CT]
296 AGGACCC
EaeI 39 CGGCCA [CT]^GGCC[GA]
149 TGGCCA
EarI 46 CTCTTC CTCTTC(1/4)
110 GAAGAG
EcoO109I 256 AGGGCCC [GA]G^G[ACGT]CC[CT]
296 AGGACCC
FokI 162 GGATG GGATG(9/13)
195 GGATG
Ksp632I 46 CTCTTC CTCTTC(1/4)
110 GAAGAG
MseI 563 TTAA T^TAA
658 TTAA
PflFI 199 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
298 GACCCGGTC
RsaI 60 GTAC GT^AC
315 GTAC
Tru9I 563 TTAA T^TAA
658 TTAA
Tsp509I 672 AATT ^AATT
693 AATT
Tth111I 199 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
298 GACCCGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI BcnI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstNI
BstUI CfoI Cfr13I DpnII EcoRII
HaeIII HapII HhaI HinP1I HpaII
HphI MboI MboII MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TaqI TspRI TthHB8I