################################## # NO CUT ENZYMES ( 195 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MroI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AciI 4 CCGC CCGC(-3/-1) AlwNI 356 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 7 CCTAAGG CC^T[ACGT]AGG AvaII 104 GGTCC G^G[AT]CC BalI 248 TGGCCA TGG^CCA BcnI 77 CCGGG CC^[GC]GG BpmI 353 CTCCAG CTGGAG(16/14) BsaI 216 GGTCTC GGTCTC(1/5) BsmFI 122 GGGAC GGGAC(10/14) Bsu36I 7 CCTAAGG CC^T[ACGT]AGG Cfr13I 104 GGTCC G^G[ACGT]CC Eco81I 7 CCTAAGG CC^T[ACGT]AGG EcoT14I 338 CCAAGG C^C[AT][AT]GG HapII 77 CCGG C^CGG HgaI 31 GACGC GACGC(5/10) HinfI 234 GATTC G^A[ACGT]TC HpaII 77 CCGG C^CGG HphI 364 GGTGA GGTGA(8/7) MluNI 248 TGGCCA TGG^CCA MnlI 239 GAGG CCTC(7/6) MscI 248 TGGCCA TGG^CCA MspI 77 CCGG C^CGG NciI 77 CCGGG CC^[GC]GG NlaIV 241 GGCTCC GG[ACGT]^[ACGT]CC PflMI 355 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Sau96I 104 GGTCC G^G[ACGT]CC SmlI 314 CTCAAG C^T[CT][GA]AG StyI 338 CCAAGG C^C[AT][AT]GG TaqI 237 TCGA T^CGA TfiI 234 GATTC G^A[AT]TC Tsp509I 321 AATT ^AATT TspRI 65 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 237 TCGA T^CGA Van91I 355 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AspHI 268 GAGCTC G[AT]GC[AT]^C 311 GAGCTC BanII 268 GAGCTC G[GA]GC[CT]^C 311 GAGCTC BbvI 256 GCAGC GCAGC(8/12) 358 GCTGC BmyI 268 GAGCTC G[AGT]GC[ACT]^C 311 GAGCTC BsiHKAI 268 GAGCTC G[AT]GC[AT]^C 311 GAGCTC BsmAI 225 GAGAC GTCTC(1/5) 217 GTCTC Bsp1286I 268 GAGCTC G[AGT]GC[ACT]^C 311 GAGCTC BsrI 294 ACTGG ACTGG(1/-1) 64 CCAGT BstNI 246 CCTGG CC^[AT]GG 251 CCAGG EaeI 44 CGGCCA [CT]^GGCC[GA] 248 TGGCCA EcoRII 246 CCTGG ^CC[AT]GG 251 CCAGG MspA1I 209 CAGCTG C[AC]G^C[GT]G 356 CAGCTG MvaI 246 CCTGG CC^[AT]GG 251 CCAGG MwoI 250 GCCAGGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 352 GCTCCAGCTGC PvuII 209 CAGCTG CAG^CTG 356 CAGCTG SacI 268 GAGCTC GAGCT^C 311 GAGCTC SfaNI 349 GATGC GCATC(5/9) 370 GATGC TseI 256 GCAGC G^C[AT]GC 358 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AluI BsaJI BsiYI BslI BssKI DdeI Fnu4HI HaeIII ItaI MboII ScrFI