Restriction map for pF1KB6787
##################################
# NO CUT ENZYMES (  195 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MboI        MfeI        MflI        MluI        MroI        
MseI        MslI        MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsaI        RsrII       SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       Tsp45I      Tth111I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AciI            4  CCGC                 CCGC(-3/-1)
AlwNI         356  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI            7  CCTAAGG              CC^T[ACGT]AGG
AvaII         104  GGTCC                G^G[AT]CC
BalI          248  TGGCCA               TGG^CCA
BcnI           77  CCGGG                CC^[GC]GG
BpmI          353  CTCCAG               CTGGAG(16/14)
BsaI          216  GGTCTC               GGTCTC(1/5)
BsmFI         122  GGGAC                GGGAC(10/14)
Bsu36I          7  CCTAAGG              CC^T[ACGT]AGG
Cfr13I        104  GGTCC                G^G[ACGT]CC
Eco81I          7  CCTAAGG              CC^T[ACGT]AGG
EcoT14I       338  CCAAGG               C^C[AT][AT]GG
HapII          77  CCGG                 C^CGG
HgaI           31  GACGC                GACGC(5/10)
HinfI         234  GATTC                G^A[ACGT]TC
HpaII          77  CCGG                 C^CGG
HphI          364  GGTGA                GGTGA(8/7)
MluNI         248  TGGCCA               TGG^CCA
MnlI          239  GAGG                 CCTC(7/6)
MscI          248  TGGCCA               TGG^CCA
MspI           77  CCGG                 C^CGG
NciI           77  CCGGG                CC^[GC]GG
NlaIV         241  GGCTCC               GG[ACGT]^[ACGT]CC
PflMI         355  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I        104  GGTCC                G^G[ACGT]CC
SmlI          314  CTCAAG               C^T[CT][GA]AG
StyI          338  CCAAGG               C^C[AT][AT]GG
TaqI          237  TCGA                 T^CGA
TfiI          234  GATTC                G^A[AT]TC
Tsp509I       321  AATT                 ^AATT
TspRI          65  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       237  TCGA                 T^CGA
Van91I        355  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AspHI         268  GAGCTC               G[AT]GC[AT]^C
              311  GAGCTC              
BanII         268  GAGCTC               G[GA]GC[CT]^C
              311  GAGCTC              
BbvI          256  GCAGC                GCAGC(8/12)
              358  GCTGC               
BmyI          268  GAGCTC               G[AGT]GC[ACT]^C
              311  GAGCTC              
BsiHKAI       268  GAGCTC               G[AT]GC[AT]^C
              311  GAGCTC              
BsmAI         225  GAGAC                GTCTC(1/5)
              217  GTCTC               
Bsp1286I      268  GAGCTC               G[AGT]GC[ACT]^C
              311  GAGCTC              
BsrI          294  ACTGG                ACTGG(1/-1)
               64  CCAGT               
BstNI         246  CCTGG                CC^[AT]GG
              251  CCAGG               
EaeI           44  CGGCCA               [CT]^GGCC[GA]
              248  TGGCCA              
EcoRII        246  CCTGG                ^CC[AT]GG
              251  CCAGG               
MspA1I        209  CAGCTG               C[AC]G^C[GT]G
              356  CAGCTG              
MvaI          246  CCTGG                CC^[AT]GG
              251  CCAGG               
MwoI          250  GCCAGGGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              352  GCTCCAGCTGC         
PvuII         209  CAGCTG               CAG^CTG
              356  CAGCTG              
SacI          268  GAGCTC               GAGCT^C
              311  GAGCTC              
SfaNI         349  GATGC                GCATC(5/9)
              370  GATGC               
TseI          256  GCAGC                G^C[AT]GC
              358  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AluI        BsaJI       BsiYI       BslI        BssKI       
DdeI        Fnu4HI      HaeIII      ItaI        MboII       
ScrFI       
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