Restriction map for
pF1KB3398
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AluI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DrdI DsaI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT14I FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MluI
MroI MslI MspA1I MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI TspRI Tth111I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AfaI 187 GTAC GT^AC
AflII 223 CTTAAG C^TTAAG
AhdI 56 GACGCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 56 GACGCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 19 CCCGGG C^[CT]CG[GA]G
BalI 119 TGGCCA TGG^CCA
BbvI 59 GCAGC GCAGC(8/12)
BfrI 223 CTTAAG C^TTAAG
BglII 258 AGATCT A^GATCT
BsiYI 19 CCCGGGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 19 CCCGGGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 19 CCCGGG C^[CT]CG[GA]G
BstBI 293 TTCGAA TT^CGAA
BstF5I 327 GGATG GGATG(2/0)
BstNI 444 CCTGG CC^[AT]GG
BstYI 258 AGATCT [GA]^GATC[CT]
DraIII 170 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 119 TGGCCA [CT]^GGCC[GA]
Eam1105I 56 GACGCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 218 GAATTC G^AATTC
EcoRII 444 CCTGG ^CC[AT]GG
EcoT22I 10 ATGCAT ATGCA^T
Fnu4HI 59 GCAGC GC^[ACGT]GC
FokI 327 GGATG GGATG(9/13)
HincII 81 GTTGAC GT[CT]^[GA]AC
HindII 81 GTTGAC GT[CT]^[GA]AC
HphI 366 TCACC GGTGA(8/7)
ItaI 59 GCAGC GC^[ACGT]GC
MaeIII 196 GTGAC ^GT[ACGT]AC
MfeI 411 CAATTG C^AATTG
MflI 258 AGATCT [GA]^GATC[CT]
MluNI 119 TGGCCA TGG^CCA
MscI 119 TGGCCA TGG^CCA
MunI 411 CAATTG C^AATTG
MvaI 444 CCTGG CC^[AT]GG
MwoI 442 GCCCTGGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 10 ATGCAT ATGCA^T
NspV 293 TTCGAA TT^CGAA
PacI 271 TTAATTAA TTAAT^TAA
PleI 398 GAGTC GAGTC(4/5)
RsaI 187 GTAC GT^AC
SfcI 431 CTACAG C^T[GA][CT]AG
SfuI 293 TTCGAA TT^CGAA
SmaI 19 CCCGGG CCC^GGG
TseI 59 GCAGC G^C[AT]GC
Tsp45I 196 GTGAC ^GT[GC]AC
XhoII 258 AGATCT [GA]^GATC[CT]
XmaCI 19 CCCGGG C^CCGGG
XmaI 19 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 284 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
434 CAGCCTCTG
BcnI 19 CCCGG CC^[GC]GG
20 CCGGG
BsaJI 19 CCCGGG C^C[ACGT][ACGT]GG
443 CCCTGG
BsrI 117 ACTGG ACTGG(1/-1)
36 CCAGT
Cac8I 8 GCATGC GC[ACGT]^[ACGT]GC
12 GCATGC
Cfr13I 27 GGGCC G^G[ACGT]CC
129 GGGCC
DdeI 160 CTGAG C^T[ACGT]AG
230 CTAAG
DraII 26 AGGGCCT [GA]G^G[ACGT]CC[CT]
128 AGGGCCT
EcoO109I 26 AGGGCCT [GA]G^G[ACGT]CC[CT]
128 AGGGCCT
HapII 20 CCGG C^CGG
360 CCGG
HgaI 56 GACGC GACGC(5/10)
62 GCGTC
HpaII 20 CCGG C^CGG
360 CCGG
MaeII 95 ACGT A^CGT
189 ACGT
MboII 256 GAAGA GAAGA(8/7)
249 TCTTC
MspI 20 CCGG C^CGG
360 CCGG
NciI 19 CCCGG CC^[GC]GG
20 CCGGG
NspI 8 GCATGC [GA]CATG^[CT]
12 GCATGC
Sau96I 27 GGGCC G^G[ACGT]CC
129 GGGCC
SfaNI 328 GATGC GCATC(5/9)
300 GCATC
SmlI 223 CTTAAG C^T[CT][GA]AG
304 CTTGAG
SphI 8 GCATGC GCATG^C
12 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AcsI ApoI BsmFI BssKI DpnII
HaeIII HinfI MboI MnlI MseI
NdeII NlaIII Sau3AI ScrFI TaqI
TfiI Tru9I Tsp509I TthHB8I