Restriction map for
pF1KB5213
##################################
# NO CUT ENZYMES ( 204 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRII
EcoRV EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HgaI HhaI
HinP1I HincII HindII HinfI HpaI
HphI ItaI KasI KpnI Ksp632I
KspI MaeI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI MvaI MvnI NaeI
NarI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI TseI Tsp45I
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 368 GACGTC GACGT^C
AciI 4 GCGG CCGC(-3/-1)
AcsI 330 AAATTC [GA]^AATT[CT]
AcyI 368 GACGTC G[GA]^CG[CT]C
ApoI 330 AAATTC [GA]^AATT[CT]
AseI 256 ATTAAT AT^TAAT
AsnI 256 ATTAAT AT^TAAT
AvaII 208 GGACC G^G[AT]CC
BcnI 299 CCCGG CC^[GC]GG
BsaHI 368 GACGTC G[GA]^CG[CT]C
BsaI 6 GGTCTC GGTCTC(1/5)
BsaJI 160 CCAAGG C^C[ACGT][ACGT]GG
BseRI 227 CTCCTC GAGGAG(10/8)
BsiYI 409 CCACCAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 409 CCACCAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 7 GTCTC GTCTC(1/5)
BssKI 299 CCCGG ^CC[ACGT]GG
BstXI 281 CCAGTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 364 CTAAG C^T[ACGT]AG
DraII 98 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 98 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 160 CCAAGG C^C[AT][AT]GG
HapII 300 CCGG C^CGG
Hin1I 368 GACGTC G[GA]^CG[CT]C
HindIII 397 AAGCTT A^AGCTT
HpaII 300 CCGG C^CGG
MaeII 369 ACGT A^CGT
MaeIII 247 GTAAC ^GT[ACGT]AC
MslI 306 CATACCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 300 CCGG C^CGG
MwoI 399 GCTTCCACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 299 CCCGG CC^[GC]GG
NlaIII 144 CATG CATG^
NlaIV 98 GGGGCC GG[ACGT]^[ACGT]CC
PshBI 256 ATTAAT AT^TAAT
ScrFI 299 CCCGG CC^[ACGT]GG
SfaNI 295 GATGC GCATC(5/9)
StyI 160 CCAAGG C^C[AT][AT]GG
XcmI 281 CCAGTGGGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 8 enzymes) #
###################################
AfaI 86 GTAC GT^AC
352 GTAC
BsrI 281 CCAGT ACTGG(1/-1)
310 CCAGT
BstF5I 287 GGATG GGATG(2/0)
294 GGATG
FokI 287 GGATG GGATG(9/13)
294 GGATG
HaeIII 100 GGCC GG^CC
155 GGCC
MnlI 28 CCTC CCTC(7/6)
229 CCTC
RsaI 86 GTAC GT^AC
352 GTAC
TspRI 282 CAGTG CA[GC]TG[ACGT][ACGT]^
311 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
AluI Cfr13I MboII MseI Sau96I
Tru9I Tsp509I