Restriction map for
pF1KB5758
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BfrI BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstPI
BstXI Bsu36I CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV EcoT22I FseI FspI Hin1I
KasI KpnI KspI MamI MluI
MroI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AseI 1201 ATTAAT AT^TAAT
AsnI 1201 ATTAAT AT^TAAT
BalI 576 TGGCCA TGG^CCA
BanI 192 GGCACC G^G[CT][GA]CC
BanII 289 GAGCTC G[GA]GC[CT]^C
BcgI 128 CGATCTCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2166 GTATCC GTATCC(6/5)
BglI 60 GCCGCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 286 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 286 GCTGAGC GC^T[ACGT]AGC
BsaI 888 GAGACC GGTCTC(1/5)
BsgI 899 CTGCAC GTGCAG(16/14)
BspDI 532 ATCGAT AT^CGAT
BsrFI 98 GCCGGT [GA]^CCGG[CT]
BssSI 1418 CACGAG CACGAG(-5/-1)
Bst1107I 1755 GTATAC GTA^TAC
BstZ17I 1755 GTATAC GTA^TAC
CelII 286 GCTGAGC GC^T[ACGT]AGC
Cfr10I 98 GCCGGT [GA]^CCGG[CT]
ClaI 532 ATCGAT AT^CGAT
DraI 564 TTTAAA TTT^AAA
EcoNI 1248 CCTCACGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 945 CCAAGG C^C[AT][AT]GG
HaeII 1967 GGCGCT [GA]GCGC^[CT]
HgaI 12 GCGTC GACGC(5/10)
HincII 1650 GTTAAC GT[CT]^[GA]AC
HindII 1650 GTTAAC GT[CT]^[GA]AC
HindIII 407 AAGCTT A^AGCTT
HpaI 1650 GTTAAC GTT^AAC
MluNI 576 TGGCCA TGG^CCA
MscI 576 TGGCCA TGG^CCA
MslI 1838 CATTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1146 CAGCGG C[AC]G^C[GT]G
NheI 204 GCTAGC G^CTAGC
NspI 872 ACATGC [GA]CATG^[CT]
PflMI 1095 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1201 ATTAAT AT^TAAT
SacI 289 GAGCTC GAGCT^C
SfcI 1826 CTGTAG C^T[GA][CT]AG
SmlI 885 CTTGAG C^T[CT][GA]AG
SspI 478 AATATT AAT^ATT
StyI 945 CCAAGG C^C[AT][AT]GG
Van91I 1095 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 882 GTAC GT^AC
1783 GTAC
AvaII 101 GGTCC G^G[AT]CC
2061 GGACC
BbvCI 105 CCTCAGC CCTCAGC(-5/-2)
178 GCTGAGG
BcnI 65 CCCGG CC^[GC]GG
152 CCCGG
BfaI 205 CTAG C^TAG
2125 CTAG
BseRI 181 GAGGAG GAGGAG(10/8)
387 GAGGAG
BsrBI 41 CCGCTC CCGCTC(-3/-3)
1157 CCGCTC
EaeI 576 TGGCCA [CT]^GGCC[GA]
1230 TGGCCG
EarI 384 GAAGAG CTCTTC(1/4)
1674 GAAGAG
EcoRI 1033 GAATTC G^AATTC
1615 GAATTC
Ksp632I 384 GAAGAG CTCTTC(1/4)
1674 GAAGAG
MaeI 205 CTAG C^TAG
2125 CTAG
MaeII 269 ACGT A^CGT
614 ACGT
MfeI 1508 CAATTG C^AATTG
1622 CAATTG
MunI 1508 CAATTG C^AATTG
1622 CAATTG
NciI 65 CCCGG CC^[GC]GG
152 CCCGG
PleI 2068 GACTC GAGTC(4/5)
1025 GAGTC
RsaI 882 GTAC GT^AC
1783 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccI AccII AciI AcsI AluI
AlwI AlwNI ApoI Asp700I AspHI
BbvI BclI BglII BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI