Restriction map for
pF1KB0485
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BbeI
BcgI BciVI BclI BfrI BlnI
BsaBI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MseI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfoI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 241 GGCGTC G[GA]^CG[CT]C
AhdI 666 GACACCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 666 GACACCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1576 GTGCTC G[AT]GC[AT]^C
BbrPI 1483 CACGTG CAC^GTG
BbsI 825 GTCTTC GAAGAC(2/6)
BbvCI 27 CCTCAGC CCTCAGC(-5/-2)
BglII 786 AGATCT A^GATCT
BlpI 561 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 561 GCTCAGC GC^T[ACGT]AGC
BpuAI 825 GTCTTC GAAGAC(2/6)
BsaHI 241 GGCGTC G[GA]^CG[CT]C
BsiHKAI 1576 GTGCTC G[AT]GC[AT]^C
BsmI 886 GCATTC GAATGC(1/-1)
BsrBI 1548 CCGCTC CCGCTC(-3/-3)
BssSI 774 CTCGTG CACGAG(-5/-1)
BstAPI 1488 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 786 AGATCT [GA]^GATC[CT]
CelII 561 GCTCAGC GC^T[ACGT]AGC
DraIII 771 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 165 CGGCCG C^GGCCG
Eam1105I 666 GACACCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 165 CGGCCG C^GGCCG
Eco52I 165 CGGCCG C^GGCCG
EcoNI 27 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 740 AGCGCC [GA]GCGC^[CT]
Hin1I 241 GGCGTC G[GA]^CG[CT]C
MflI 786 AGATCT [GA]^GATC[CT]
PmaCI 1483 CACGTG CAC^GTG
PmlI 1483 CACGTG CAC^GTG
PpuMI 119 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 993 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1477 CAGCTG CAG^CTG
SapI 950 GCTCTTC GCTCTTC(1/4)
SfiI 1197 GGCCATCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1465 CGCCGGCG C[GA]^CCGG[CT]G
StuI 1256 AGGCCT AGG^CCT
TaqI 257 TCGA T^CGA
TthHB8I 257 TCGA T^CGA
XcmI 1079 CCACTGGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 786 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
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AfaI 363 GTAC GT^AC
1221 GTAC
AluI 286 AGCT AG^CT
1478 AGCT
AlwI 1063 GATCC GGATC(4/5)
1230 GATCC
AlwNI 59 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1489 CAGGGCCTG
BalI 1145 TGGCCA TGG^CCA
1568 TGGCCA
BfaI 676 CTAG C^TAG
812 CTAG
BglI 1085 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1198 GCCATCGTGGC
BpmI 315 CTCCAG CTGGAG(16/14)
638 CTGGAG
BsaAI 1222 TACGTG [CT]AC^GT[GA]
1483 CACGTG
BsaI 919 GAGACC GGTCTC(1/5)
1174 GAGACC
BseRI 457 CTCCTC GAGGAG(10/8)
109 GAGGAG
BspMI 985 ACCTGC ACCTGC(4/8)
1280 ACCTGC
BstXI 317 CCAGTTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
581 CCATTCCGGTGG
DrdI 414 GACACTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1176 GACCCTCCTGTC
EarI 951 CTCTTC CTCTTC(1/4)
1108 CTCTTC
EcoT14I 724 CCAAGG C^C[AT][AT]GG
912 CCTTGG
HgaI 1241 GACGC GACGC(5/10)
242 GCGTC
Ksp632I 951 CTCTTC CTCTTC(1/4)
1108 CTCTTC
MaeI 676 CTAG C^TAG
812 CTAG
MaeII 1223 ACGT A^CGT
1484 ACGT
MluNI 1145 TGGCCA TGG^CCA
1568 TGGCCA
MscI 1145 TGGCCA TGG^CCA
1568 TGGCCA
NaeI 1034 GCCGGC GCC^GGC
1466 GCCGGC
NgoMIV 1034 GCCGGC G^CCGGC
1466 GCCGGC
PstI 139 CTGCAG CTGCA^G
1378 CTGCAG
RsaI 363 GTAC GT^AC
1221 GTAC
SfcI 139 CTGCAG C^T[GA][CT]AG
1378 CTGCAG
StyI 724 CCAAGG C^C[AT][AT]GG
912 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AvaI AvaII BanI
BanII BbvI BcnI BmyI BsaJI
BsaWI BsgI BsiEI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI Sau3AI
Sau96I ScrFI SfaNI SmaI TseI
Tsp45I TspRI Van91I XmaCI XmaI