Restriction map for
pF1KE0722
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BsrI BssHII
BssSI BstBI BstYI CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI KasI KpnI MaeI
MaeII MamI MfeI MflI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Van91I
XbaI XcmI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 781 GACGCC G[GA]^CG[CT]C
Alw44I 694 GTGCAC G^TGCAC
AlwI 1161 GATCC GGATC(4/5)
AlwNI 971 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 213 CCTGAGG CC^T[ACGT]AGG
ApaI 134 GGGCCC GGGCC^C
ApaLI 694 GTGCAC G^TGCAC
AspI 367 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 582 TGCGCA TGC^GCA
BalI 192 TGGCCA TGG^CCA
BanI 840 GGTGCC G^G[CT][GA]CC
BbsI 420 GAAGAC GAAGAC(2/6)
BglI 547 GCCACCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 420 GAAGAC GAAGAC(2/6)
BsaHI 781 GACGCC G[GA]^CG[CT]C
BsaI 991 GAGACC GGTCTC(1/5)
BsiEI 861 CGATCG CG[GA][CT]^CG
BsrDI 579 CATTGC GCAATG(2/0)
BsrFI 662 ACCGGC [GA]^CCGG[CT]
Bst1107I 565 GTATAC GTA^TAC
BstAPI 970 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 870 GGTCACC G^GT[ACGT]ACC
BstPI 870 GGTCACC G^GT[ACGT]ACC
BstXI 959 CCACTTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 565 GTATAC GTA^TAC
Bsu36I 213 CCTGAGG CC^T[ACGT]AGG
Cfr10I 662 ACCGGC [GA]^CCGG[CT]
Eco81I 213 CCTGAGG CC^T[ACGT]AGG
EcoNI 288 CCTGCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 870 GGTCACC G^GT[ACGT]ACC
EcoT14I 407 CCATGG C^C[AT][AT]GG
FspI 582 TGCGCA TGC^GCA
Hin1I 781 GACGCC G[GA]^CG[CT]C
HindIII 1240 AAGCTT A^AGCTT
KspI 1139 CCGCGG CCGC^GG
MaeIII 871 GTCAC ^GT[ACGT]AC
MluNI 192 TGGCCA TGG^CCA
MscI 192 TGGCCA TGG^CCA
MslI 1038 CATCGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 407 CCATGG C^CATGG
PaeR7I 310 CTCGAG C^TCGAG
PflFI 367 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 861 CGATCG CGAT^CG
SacI 736 GAGCTC GAGCT^C
SacII 1139 CCGCGG CCGC^GG
SapI 378 GAAGAGC GCTCTTC(1/4)
SfaNI 22 GATGC GCATC(5/9)
SmlI 310 CTCGAG C^T[CT][GA]AG
StyI 407 CCATGG C^C[AT][AT]GG
Tsp45I 871 GTCAC ^GT[GC]AC
Tth111I 367 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 310 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccI 565 GTATAC GT^[AC][GT]AC
1027 GTCTAC
AfaI 153 GTAC GT^AC
1086 GTAC
AvaI 310 CTCGAG C^[CT]CG[GA]G
1009 CCCGAG
BsaWI 623 ACCGGA [AT]^CCGG[AT]
837 TCCGGT
BsoBI 310 CTCGAG C^[CT]CG[GA]G
1009 CCCGAG
BspMI 187 GCAGGT ACCTGC(4/8)
501 GCAGGT
DraII 133 AGGGCCC [GA]G^G[ACGT]CC[CT]
217 AGGGCCT
DsaI 407 CCATGG C^C[GA][CT]GG
1139 CCGCGG
EarI 378 GAAGAG CTCTTC(1/4)
510 GAAGAG
EcoO109I 133 AGGGCCC [GA]G^G[ACGT]CC[CT]
217 AGGGCCT
HaeII 481 AGCGCC [GA]GCGC^[CT]
1092 GGCGCT
Ksp632I 378 GAAGAG CTCTTC(1/4)
510 GAAGAG
RsaI 153 GTAC GT^AC
1086 GTAC
StuI 464 AGGCCT AGG^CCT
638 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII Sau3AI Sau96I
ScrFI SfcI TaqI TseI TspRI
TthHB8I