################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeIII MamI MluI MluNI MroI MscI NaeI NarI NcoI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tsp45I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccII 156 CGCG CG^CG AseI 900 ATTAAT AT^TAAT AsnI 900 ATTAAT AT^TAAT AspHI 500 GAGCAC G[AT]GC[AT]^C BcnI 1083 CCCGG CC^[GC]GG BglII 1130 AGATCT A^GATCT BseRI 497 GAGGAG GAGGAG(10/8) BsiHKAI 500 GAGCAC G[AT]GC[AT]^C BspDI 38 ATCGAT AT^CGAT BspLU11I 138 ACATGT A^CATGT BstUI 156 CGCG CG^CG ClaI 38 ATCGAT AT^CGAT DraI 795 TTTAAA TTT^AAA EaeI 1197 CGGCCA [CT]^GGCC[GA] HapII 1084 CCGG C^CGG HgaI 154 GACGC GACGC(5/10) HinfI 934 GAATC G^A[ACGT]TC HpaII 1084 CCGG C^CGG MaeII 521 ACGT A^CGT MfeI 224 CAATTG C^AATTG MslI 503 CACTATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1181 CCGCTG C[AC]G^C[GT]G MspI 1084 CCGG C^CGG MunI 224 CAATTG C^AATTG MvnI 156 CGCG CG^CG NciI 1083 CCCGG CC^[GC]GG NdeI 779 CATATG CA^TATG NheI 687 GCTAGC G^CTAGC PflMI 411 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 900 ATTAAT AT^TAAT SphI 1006 GCATGC GCATG^C SspI 83 AATATT AAT^ATT TfiI 934 GAATC G^A[AT]TC Van91I 411 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1220 CCACTATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 283 AAATTT [GA]^AATT[CT] 784 GAATTT AflIII 138 ACATGT A^C[GA][CT]GT 521 ACGTGT ApoI 283 AAATTT [GA]^AATT[CT] 784 GAATTT BmyI 500 GAGCAC G[AGT]GC[ACT]^C 1216 GTGCCC BsgI 480 GTGCAG GTGCAG(16/14) 883 GTGCAG BsmBI 91 GAGACG CGTCTC(1/5) 1193 GAGACG Bsp1286I 500 GAGCAC G[AGT]GC[ACT]^C 1216 GTGCCC BspMI 1112 ACCTGC ACCTGC(4/8) 1329 ACCTGC BstYI 645 GGATCT [GA]^GATC[CT] 1130 AGATCT CfoI 591 GCGC GCG^C 927 GCGC HhaI 591 GCGC GCG^C 927 GCGC HinP1I 591 GCGC G^CGC 927 GCGC HphI 462 GGTGA GGTGA(8/7) 408 TCACC MflI 645 GGATCT [GA]^GATC[CT] 1130 AGATCT PpuMI 1014 AGGACCC [GA]G^G[AT]CC[CT] 1078 GGGACCC SmlI 47 CTCAAG C^T[CT][GA]AG 260 CTCAAG StuI 101 AGGCCT AGG^CCT 1313 AGGCCT XhoII 645 GGATCT [GA]^GATC[CT] 1130 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbvI BfaI BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII ItaI Ksp632I MaeI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI Tsp509I TspRI TthHB8I