Restriction map for
pF1KE4539
##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AviII BbeI
BcgI BciVI BclI BfrI BglII
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI DraIII DrdI Eco47III EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SbfI ScaI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I XbaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 1316 GGTACC G^GTACC
AccI 711 GTCGAC GT^[AC][GT]AC
AfaI 1317 GTAC GT^AC
AhdI 897 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1316 GGTACC G^GTACC
AspEI 897 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1809 CCTAGG C^CTAGG
BamHI 1685 GGATCC G^GATCC
BbsI 894 GAAGAC GAAGAC(2/6)
BlnI 1809 CCTAGG C^CTAGG
BpuAI 894 GAAGAC GAAGAC(2/6)
BspHI 1568 TCATGA T^CATGA
BsrBI 1660 GAGCGG CCGCTC(-3/-3)
BsrFI 317 ACCGGC [GA]^CCGG[CT]
BstEII 1087 GGTTACC G^GT[ACGT]ACC
BstPI 1087 GGTTACC G^GT[ACGT]ACC
Cfr10I 317 ACCGGC [GA]^CCGG[CT]
Eam1105I 897 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 642 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1087 GGTTACC G^GT[ACGT]ACC
KpnI 1316 GGTACC GGTAC^C
KspI 871 CCGCGG CCGC^GG
NspI 1775 GCATGC [GA]CATG^[CT]
PaeR7I 99 CTCGAG C^TCGAG
PstI 679 CTGCAG CTGCA^G
RcaI 1568 TCATGA T^CATGA
RsaI 1317 GTAC GT^AC
SacII 871 CCGCGG CCGC^GG
SalI 711 GTCGAC G^TCGAC
SapI 72 GCTCTTC GCTCTTC(1/4)
SexAI 1757 ACCTGGT A^CC[AT]GGT
SmaI 633 CCCGGG CCC^GGG
SphI 1775 GCATGC GCATG^C
TspRI 1841 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 1748 CCAACGGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 99 CTCGAG C^TCGAG
XmaCI 633 CCCGGG C^CCGGG
XmaI 633 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 1397 ACACGT A^C[GA][CT]GT
1399 ACGTGT
AocI 1141 CCTGAGG CC^T[ACGT]AGG
1225 CCTCAGG
ApaI 397 GGGCCC GGGCC^C
1505 GGGCCC
AspI 145 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
714 GACATGGTC
BanI 1316 GGTACC G^G[CT][GA]CC
1664 GGCACC
BbrPI 1398 CACGTG CAC^GTG
1636 CACGTG
BlpI 528 GCTGAGC GC^T[ACGT]AGC
1344 GCTGAGC
BpmI 113 CTGGAG CTGGAG(16/14)
1174 CTGGAG
Bpu1102I 528 GCTGAGC GC^T[ACGT]AGC
1344 GCTGAGC
BsaAI 1398 CACGTG [CT]AC^GT[GA]
1636 CACGTG
BsaI 1177 GAGACC GGTCTC(1/5)
521 GGTCTC
BstXI 192 CCAACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
998 CCACCCAGATGG
BstYI 1484 AGATCC [GA]^GATC[CT]
1685 GGATCC
Bsu36I 1141 CCTGAGG CC^T[ACGT]AGG
1225 CCTCAGG
CelII 528 GCTGAGC GC^T[ACGT]AGC
1344 GCTGAGC
DsaI 871 CCGCGG C^C[GA][CT]GG
1511 CCACGG
EagI 599 CGGCCG C^GGCCG
868 CGGCCG
EarI 73 CTCTTC CTCTTC(1/4)
1123 CTCTTC
EclXI 599 CGGCCG C^GGCCG
868 CGGCCG
Eco52I 599 CGGCCG C^GGCCG
868 CGGCCG
Eco81I 1141 CCTGAGG CC^T[ACGT]AGG
1225 CCTCAGG
HaeII 581 GGCGCT [GA]GCGC^[CT]
1527 GGCGCT
Ksp632I 73 CTCTTC CTCTTC(1/4)
1123 CTCTTC
MflI 1484 AGATCC [GA]^GATC[CT]
1685 GGATCC
MslI 999 CACCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1563 CACGTTCATG
PflFI 145 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
714 GACATGGTC
PflMI 1107 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1386 CCAGGACCTGG
PmaCI 1398 CACGTG CAC^GTG
1636 CACGTG
PmlI 1398 CACGTG CAC^GTG
1636 CACGTG
SacI 1072 GAGCTC GAGCT^C
1347 GAGCTC
SfcI 679 CTGCAG C^T[GA][CT]AG
1732 CTACAG
SfiI 591 GGCCGAGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
987 GGCCCTGCTGGCC
SmlI 99 CTCGAG C^T[CT][GA]AG
1129 CTCAAG
StuI 404 AGGCCT AGG^CCT
558 AGGCCT
Tsp45I 1165 GTGAC ^GT[GC]AC
1403 GTGAC
Tth111I 145 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
714 GACATGGTC
Van91I 1107 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1386 CCAGGACCTGG
XhoII 1484 AGATCC [GA]^GATC[CT]
1685 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AatII AccII AciI AcyI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI TthHB8I