Restriction map for
pF1KB9508
##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AflIII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
Bst1107I BstAPI BstBI BstYI BstZ17I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MseI MspA1I MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 525 TCCGGA T^CCGGA
AluI 269 AGCT AG^CT
Alw44I 130 GTGCAC G^TGCAC
AlwNI 182 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 187 CCTGAGG CC^T[ACGT]AGG
ApaI 241 GGGCCC GGGCC^C
ApaLI 130 GTGCAC G^TGCAC
AvaI 951 CCCGAG C^[CT]CG[GA]G
BalI 1022 TGGCCA TGG^CCA
BanI 565 GGTGCC G^G[CT][GA]CC
BbsI 55 GTCTTC GAAGAC(2/6)
BpmI 106 CTGGAG CTGGAG(16/14)
BpuAI 55 GTCTTC GAAGAC(2/6)
BsaAI 667 TACGTG [CT]AC^GT[GA]
BsaI 12 GAGACC GGTCTC(1/5)
BsaWI 525 TCCGGA [AT]^CCGG[AT]
BseAI 525 TCCGGA T^CCGGA
BseRI 27 GAGGAG GAGGAG(10/8)
BsgI 290 CTGCAC GTGCAG(16/14)
BsiEI 543 CGACCG CG[GA][CT]^CG
BsmAI 12 GAGAC GTCTC(1/5)
BsmI 968 GCATTC GAATGC(1/-1)
BsoBI 951 CCCGAG C^[CT]CG[GA]G
BspEI 525 TCCGGA T^CCGGA
BssHII 601 GCGCGC G^CGCGC
Bsu36I 187 CCTGAGG CC^T[ACGT]AGG
DdeI 188 CTGAG C^T[ACGT]AG
DrdI 544 GACCGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 187 CCTGAGG CC^T[ACGT]AGG
EcoT14I 926 CCATGG C^C[AT][AT]GG
MluNI 1022 TGGCCA TGG^CCA
MroI 525 TCCGGA T^CCGGA
MscI 1022 TGGCCA TGG^CCA
NaeI 869 GCCGGC GCC^GGC
NcoI 926 CCATGG C^CATGG
NgoMIV 869 GCCGGC G^CCGGC
NlaIII 927 CATG CATG^
PflMI 874 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 959 CTGCAG CTGCA^G
SfcI 959 CTGCAG C^T[GA][CT]AG
StyI 926 CCATGG C^C[AT][AT]GG
TfiI 143 GATTC G^A[AT]TC
Van91I 874 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 220 GTCTAC GT^[AC][GT]AC
394 GTCTAC
AspHI 130 GTGCAC G[AT]GC[AT]^C
324 GAGCAC
BanII 241 GGGCCC G[GA]GC[CT]^C
693 GGGCTC
BcnI 239 CCGGG CC^[GC]GG
738 CCCGG
BsiHKAI 130 GTGCAC G[AT]GC[AT]^C
324 GAGCAC
BspMI 844 ACCTGC ACCTGC(4/8)
441 GCAGGT
BssSI 589 CTCGTG CACGAG(-5/-1)
676 CTCGTG
BstXI 584 CCAGCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
830 CCACCTTTTTGG
DsaI 926 CCATGG C^C[GA][CT]GG
1019 CCGTGG
EarI 409 CTCTTC CTCTTC(1/4)
24 GAAGAG
Ksp632I 409 CTCTTC CTCTTC(1/4)
24 GAAGAG
MaeII 668 ACGT A^CGT
794 ACGT
MslI 327 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
657 CACAGCGATG
NciI 239 CCGGG CC^[GC]GG
738 CCCGG
PleI 672 GACTC GAGTC(4/5)
64 GAGTC
PpuMI 537 AGGTCCC [GA]G^G[AT]CC[CT]
905 AGGACCT
SfaNI 625 GATGC GCATC(5/9)
18 GCATC
TaqI 941 TCGA T^CGA
972 TCGA
TthHB8I 941 TCGA T^CGA
972 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AlwI AvaII
BbvI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
Cac8I CfoI Cfr10I Cfr13I DpnII
DraII EaeI EcoO109I EcoO65I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspI MvaI MvnI MwoI NdeII
NlaIV RsaI Sau3AI Sau96I ScrFI
TseI Tsp45I TspRI