##################################
# NO CUT ENZYMES (  199 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiHKAI     BsiWI       BsmI        
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DdeI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FokI        FseI        FspI        
HaeII       HgaI        HhaI        HinP1I      HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        KspI        MaeI        MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MunI        
MvnI        MwoI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII          85  GACGTC               GACGT^C
AcyI           85  GACGTC               G[GA]^CG[CT]C
AluI          107  AGCT                 AG^CT
AvaI          143  CTCGGG               C^[CT]CG[GA]G
BcnI           21  CCCGG                CC^[GC]GG
BsaHI          85  GACGTC               G[GA]^CG[CT]C
BsiEI          44  CGATCG               CG[GA][CT]^CG
BsmBI          87  CGTCTC               CGTCTC(1/5)
BsmFI          76  GGGAC                GGGAC(10/14)
BsoBI         143  CTCGGG               C^[CT]CG[GA]G
BstXI          92  CCAGTGCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII          45  GATC                 ^GATC
DraII          76  GGGACCC              [GA]G^G[ACGT]CC[CT]
EarI            7  CTCTTC               CTCTTC(1/4)
EcoO109I       76  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT14I        98  CCTTGG               C^C[AT][AT]GG
FauI          192  GCGGG                CCCGC(4/6)
Fnu4HI        158  GCCGC                GC^[ACGT]GC
HaeIII         58  GGCC                 GG^CC
HapII          22  CCGG                 C^CGG
Hin1I          85  GACGTC               G[GA]^CG[CT]C
HinfI         244  GACTC                G^A[ACGT]TC
HpaII          22  CCGG                 C^CGG
ItaI          158  GCCGC                GC^[ACGT]GC
Ksp632I         7  CTCTTC               CTCTTC(1/4)
MaeII          86  ACGT                 A^CGT
MboI           45  GATC                 ^GATC
MboII           8  TCTTC                GAAGA(8/7)
MspI           22  CCGG                 C^CGG
NciI           21  CCCGG                CC^[GC]GG
NdeII          45  GATC                 ^GATC
PleI          244  GACTC                GAGTC(4/5)
PpuMI          76  GGGACCC              [GA]G^G[AT]CC[CT]
PvuI           44  CGATCG               CGAT^CG
SapI            6  GCTCTTC              GCTCTTC(1/4)
Sau3AI         45  GATC                 ^GATC
SmlI          235  CTCAAG               C^T[CT][GA]AG
StyI           98  CCTTGG               C^C[AT][AT]GG
TaqI           43  TCGA                 T^CGA
TthHB8I        43  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AciI          159  CCGC                 CCGC(-3/-1)
              192  GCGG                
AvaII          24  GGTCC                G^G[AT]CC
               77  GGACC               
BpmI           90  CTCCAG               CTGGAG(16/14)
              130  CTGGAG              
BsmAI         208  GAGAC                GTCTC(1/5)
               88  GTCTC               
BsrI          129  ACTGG                ACTGG(1/-1)
               92  CCAGT               
BstNI          27  CCTGG                CC^[AT]GG
               72  CCAGG               
EcoRII         27  CCTGG                ^CC[AT]GG
               72  CCAGG               
MnlI            3  GAGG                 CCTC(7/6)
              133  GAGG                
MvaI           27  CCTGG                CC^[AT]GG
               72  CCAGG               
NlaIV          76  GGGACC               GG[ACGT]^[ACGT]CC
               77  GGACCC              
SfaNI         189  GATGC                GCATC(5/9)
              161  GCATC               
TspRI          93  CAGTG                CA[GC]TG[ACGT][ACGT]^
              128  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BsaJI       BsiYI       BslI        BssKI       Cfr13I      
Sau96I      ScrFI