Restriction map for
pF1KB6683
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanII BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MflI MluI MluNI MscI MspA1I
MunI NciI NcoI NdeI NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 237 TCCGGA T^CCGGA
AcyI 73 GGCGCC G[GA]^CG[CT]C
Asp700I 502 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 163 CCCGAG C^[CT]CG[GA]G
BanI 73 GGCGCC G^G[CT][GA]CC
BbeI 73 GGCGCC GGCGC^C
BfaI 329 CTAG C^TAG
BglI 9 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 978 CTGGAG CTGGAG(16/14)
BsaHI 73 GGCGCC G[GA]^CG[CT]C
BsaI 1169 GAGACC GGTCTC(1/5)
BsaWI 237 TCCGGA [AT]^CCGG[AT]
BseAI 237 TCCGGA T^CCGGA
BsoBI 163 CCCGAG C^[CT]CG[GA]G
BspEI 237 TCCGGA T^CCGGA
BspMI 1069 GCAGGT ACCTGC(4/8)
BsrDI 735 GCAATG GCAATG(2/0)
BstXI 540 CCAATACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1153 TTTAAA TTT^AAA
DraII 295 GGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 7 TGGCCG [CT]^GGCC[GA]
EcoO109I 295 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 1226 CCAAGG C^C[AT][AT]GG
Hin1I 73 GGCGCC G[GA]^CG[CT]C
KasI 73 GGCGCC G^GCGCC
MaeI 329 CTAG C^TAG
MaeII 827 ACGT A^CGT
MroI 237 TCCGGA T^CCGGA
NarI 73 GGCGCC GG^CGCC
PflMI 606 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 295 GGGTCCC [GA]G^G[AT]CC[CT]
ScaI 1083 AGTACT AGT^ACT
SfcI 1244 CTGTAG C^T[GA][CT]AG
SfoI 73 GGCGCC GGC^GCC
SgrAI 48 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1108 CTCAAG C^T[CT][GA]AG
StyI 1226 CCAAGG C^C[AT][AT]GG
Van91I 606 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 502 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 14 CGCG CG^CG
78 CGCG
AvaII 296 GGTCC G^G[AT]CC
894 GGTCC
BbvI 36 GCTGC GCAGC(8/12)
39 GCTGC
BsgI 135 CTGCAC GTGCAG(16/14)
1189 CTGCAC
BsrFI 9 GCCGGC [GA]^CCGG[CT]
49 GCCGGC
BstF5I 644 CATCC GGATG(2/0)
966 GGATG
BstUI 14 CGCG CG^CG
78 CGCG
Cfr10I 9 GCCGGC [GA]^CCGG[CT]
49 GCCGGC
Cfr13I 296 GGTCC G^G[ACGT]CC
894 GGTCC
EcoT22I 348 ATGCAT ATGCA^T
399 ATGCAT
FokI 644 CATCC GGATG(9/13)
966 GGATG
HaeII 33 GGCGCT [GA]GCGC^[CT]
73 GGCGCC
HaeIII 8 GGCC GG^CC
538 GGCC
HphI 367 GGTGA GGTGA(8/7)
355 TCACC
MvnI 14 CGCG CG^CG
78 CGCG
NaeI 9 GCCGGC GCC^GGC
49 GCCGGC
NgoMIV 9 GCCGGC G^CCGGC
49 GCCGGC
NsiI 348 ATGCAT ATGCA^T
399 ATGCAT
Sau96I 296 GGTCC G^G[ACGT]CC
894 GGTCC
SfaNI 767 GATGC GCATC(5/9)
350 GCATC
TseI 36 GCTGC G^C[AT]GC
39 GCTGC
Tsp45I 103 GTGAC ^GT[GC]AC
385 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BsaJI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstNI Cac8I
CfoI DdeI DpnII EcoRII FauI
Fnu4HI HapII HhaI HinP1I HinfI
HpaII ItaI MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI ScrFI TfiI Tru9I
Tsp509I TspRI