Restriction map for
pF1KB6239
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AhdI AocI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BfrI BglI
BlnI BpuAI BsaAI BsaHI BseAI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRV
EcoT22I FseI FspI HaeII HgaI
Hin1I KasI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 1403 GTAGAC GT^[AC][GT]AC
AccII 232 CGCG CG^CG
AgeI 1502 ACCGGT A^CCGGT
Alw44I 202 GTGCAC G^TGCAC
AlwNI 1166 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 202 GTGCAC G^TGCAC
AseI 1123 ATTAAT AT^TAAT
AsnI 1123 ATTAAT AT^TAAT
BanI 1863 GGCACC G^G[CT][GA]CC
BbvCI 1459 GCTGAGG CCTCAGC(-5/-2)
BcgI 2074 GCAAATCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1687 CCGGG CC^[GC]GG
BglII 603 AGATCT A^GATCT
BlpI 1033 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1033 GCTGAGC GC^T[ACGT]AGC
BsaBI 1890 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 338 GGTCTC GGTCTC(1/5)
BsaWI 1502 ACCGGT [AT]^CCGG[AT]
BsmBI 569 GAGACG CGTCTC(1/5)
BsrBI 1036 GAGCGG CCGCTC(-3/-3)
BsrFI 1502 ACCGGT [GA]^CCGG[CT]
BstEII 252 GGTGACC G^GT[ACGT]ACC
BstPI 252 GGTGACC G^GT[ACGT]ACC
BstUI 232 CGCG CG^CG
CelII 1033 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1502 ACCGGT [GA]^CCGG[CT]
DraI 868 TTTAAA TTT^AAA
DsaI 1591 CCACGG C^C[GA][CT]GG
EcoO65I 252 GGTGACC G^GT[ACGT]ACC
EcoRI 1921 GAATTC G^AATTC
HincII 2119 GTTAAC GT[CT]^[GA]AC
HindII 2119 GTTAAC GT[CT]^[GA]AC
HindIII 828 AAGCTT A^AGCTT
HpaI 2119 GTTAAC GTT^AAC
MamI 1890 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 232 CGCG CG^CG
NciI 1687 CCGGG CC^[GC]GG
PflMI 990 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1502 ACCGGT A^CCGGT
PshAI 1762 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1123 ATTAAT AT^TAAT
PvuII 1534 CAGCTG CAG^CTG
SapI 2173 GAAGAGC GCTCTTC(1/4)
SspI 1282 AATATT AAT^ATT
Van91I 990 CCAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 957 TCTAGA T^CTAGA
XcmI 1121 CCATTAATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 1399 ACATGT A^C[GA][CT]GT
1997 ACATGT
BclI 1584 TGATCA T^GATCA
2127 TGATCA
BpmI 1448 CTCCAG CTGGAG(16/14)
607 CTGGAG
BsmI 303 GAATGC GAATGC(1/-1)
525 GAATGC
BspLU11I 1399 ACATGT A^CATGT
1997 ACATGT
BsrDI 227 CATTGC GCAATG(2/0)
1374 CATTGC
BstAPI 1817 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1851 GCATCTATTGC
BstXI 34 CCAGTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
257 CCAGTGACTTGG
BstYI 603 AGATCT [GA]^GATC[CT]
1902 AGATCC
CfoI 231 GCGC GCG^C
761 GCGC
EcoT14I 531 CCTTGG C^C[AT][AT]GG
1480 CCAAGG
FbaI 1584 TGATCA T^GATCA
2127 TGATCA
HapII 1503 CCGG C^CGG
1687 CCGG
HhaI 231 GCGC GCG^C
761 GCGC
HinP1I 231 GCGC G^CGC
761 GCGC
HpaII 1503 CCGG C^CGG
1687 CCGG
MaeII 1059 ACGT A^CGT
1874 ACGT
MflI 603 AGATCT [GA]^GATC[CT]
1902 AGATCC
MslI 616 CACCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1122 CATTAATGTG
MspI 1503 CCGG C^CGG
1687 CCGG
PstI 1521 CTGCAG CTGCA^G
1824 CTGCAG
SacI 222 GAGCTC GAGCT^C
1230 GAGCTC
SmlI 85 CTCAAG C^T[CT][GA]AG
1955 CTCAAG
StyI 531 CCTTGG C^C[AT][AT]GG
1480 CCAAGG
TaqI 1228 TCGA T^CGA
2231 TCGA
TfiI 1135 GAATC G^A[AT]TC
1432 GAATC
TthHB8I 1228 TCGA T^CGA
2231 TCGA
XhoII 603 AGATCT [GA]^GATC[CT]
1902 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BciVI BfaI BmyI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII FauI Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
Tru9I TseI Tsp45I Tsp509I TspRI