Restriction map for
pF1KE1037
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BglI BglII BlnI BpuAI BsaAI
BsaHI BsaI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsoBI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Cfr10I
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoT14I
FbaI FseI FspI HgaI Hin1I
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
Tth111I Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 73 enzymes) #
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AciI 1077 CCGC CCGC(-3/-1)
AcsI 253 GAATTC [GA]^AATT[CT]
AflII 1134 CTTAAG C^TTAAG
AlwI 54 GATCC GGATC(4/5)
AlwNI 1061 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 736 CCTCAGG CC^T[ACGT]AGG
ApoI 253 GAATTC [GA]^AATT[CT]
Asp700I 913 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvI 934 GCTGC GCAGC(8/12)
BfrI 1134 CTTAAG C^TTAAG
BlpI 594 GCTAAGC GC^T[ACGT]AGC
BpmI 358 CTCCAG CTGGAG(16/14)
Bpu1102I 594 GCTAAGC GC^T[ACGT]AGC
BsaBI 48 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 634 CCTGGG C^C[ACGT][ACGT]GG
BsaWI 457 ACCGGA [AT]^CCGG[AT]
BsiYI 339 CCAATACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 339 CCAATACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 887 GGGAC GGGAC(10/14)
BspDI 559 ATCGAT AT^CGAT
BspHI 959 TCATGA T^CATGA
BstXI 88 CCAGCTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 53 AGATCC [GA]^GATC[CT]
Bsu36I 736 CCTCAGG CC^T[ACGT]AGG
Cac8I 294 GCAGGC GC[ACGT]^[ACGT]GC
CelII 594 GCTAAGC GC^T[ACGT]AGC
CfoI 1029 GCGC GCG^C
Cfr13I 233 GGCCC G^G[ACGT]CC
ClaI 559 ATCGAT AT^CGAT
DpnII 54 GATC ^GATC
EaeI 1041 TGGCCG [CT]^GGCC[GA]
EarI 73 GAAGAG CTCTTC(1/4)
Eco81I 736 CCTCAGG CC^T[ACGT]AGG
EcoRI 253 GAATTC G^AATTC
EcoRV 1071 GATATC GAT^ATC
EcoT22I 407 ATGCAT ATGCA^T
FauI 1076 CCCGC CCCGC(4/6)
Fnu4HI 934 GCTGC GC^[ACGT]GC
HaeII 1028 GGCGCT [GA]GCGC^[CT]
HapII 458 CCGG C^CGG
HhaI 1029 GCGC GCG^C
HinP1I 1029 GCGC G^CGC
HincII 952 GTTGAC GT[CT]^[GA]AC
HindII 952 GTTGAC GT[CT]^[GA]AC
HindIII 597 AAGCTT A^AGCTT
HpaII 458 CCGG C^CGG
ItaI 934 GCTGC GC^[ACGT]GC
Ksp632I 73 GAAGAG CTCTTC(1/4)
MaeII 1047 ACGT A^CGT
MaeIII 670 GTCAC ^GT[ACGT]AC
MamI 48 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 54 GATC ^GATC
MflI 53 AGATCC [GA]^GATC[CT]
MspA1I 89 CAGCTG C[AC]G^C[GT]G
MspI 458 CCGG C^CGG
NdeII 54 GATC ^GATC
NsiI 407 ATGCAT ATGCA^T
NspI 405 ACATGC [GA]CATG^[CT]
PshAI 106 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 89 CAGCTG CAG^CTG
RcaI 959 TCATGA T^CATGA
SacI 355 GAGCTC GAGCT^C
Sau3AI 54 GATC ^GATC
Sau96I 233 GGCCC G^G[ACGT]CC
SspI 894 AATATT AAT^ATT
TaqI 560 TCGA T^CGA
TseI 934 GCTGC G^C[AT]GC
Tsp45I 670 GTCAC ^GT[GC]AC
TthHB8I 560 TCGA T^CGA
XbaI 1083 TCTAGA T^CTAGA
XcmI 624 CCATCATCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 53 AGATCC [GA]^GATC[CT]
XmnI 913 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 9 enzymes) #
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AspHI 117 GTGCTC G[AT]GC[AT]^C
355 GAGCTC
BsiHKAI 117 GTGCTC G[AT]GC[AT]^C
355 GAGCTC
BsmAI 104 GAGAC GTCTC(1/5)
78 GTCTC
BsmI 334 GAATGC GAATGC(1/-1)
742 GCATTC
Bsp1407I 221 TGTACA T^GTACA
810 TGTACA
BsrGI 221 TGTACA T^GTACA
810 TGTACA
PleI 452 GACTC GAGTC(4/5)
76 GAGTC
SmlI 430 CTCAAG C^T[CT][GA]AG
1134 CTTAAG
SspBI 221 TGTACA T^GTACA
810 TGTACA
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI BanII BfaI BmyI
Bsp1286I BsrI BssKI BstF5I BstNI
DdeI EcoRII FokI HaeIII HinfI
HphI MaeI MboII MnlI MseI
MslI MvaI MwoI NlaIII RsaI
ScrFI SfaNI TfiI Tru9I Tsp509I
TspRI