Restriction map for
pF1KB5057
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbrPI BbvCI BciVI BfaI BfrI
BglI BlnI BsaAI BsaBI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MseI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 1403 TCCGGA T^CCGGA
AcsI 446 GAATTC [GA]^AATT[CT]
AfaI 1330 GTAC GT^AC
AflIII 779 ACGTGT A^C[GA][CT]GT
Alw44I 613 GTGCAC G^TGCAC
AlwNI 565 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 740 GGGCCC GGGCC^C
ApaLI 613 GTGCAC G^TGCAC
ApoI 446 GAATTC [GA]^AATT[CT]
Asp700I 405 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 638 CGACAGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 338 TGATCA T^GATCA
BlpI 1177 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1177 GCTAAGC GC^T[ACGT]AGC
BsaWI 1403 TCCGGA [AT]^CCGG[AT]
BseAI 1403 TCCGGA T^CCGGA
BspEI 1403 TCCGGA T^CCGGA
BsrBI 400 GAGCGG CCGCTC(-3/-3)
BsrDI 1426 GCAATG GCAATG(2/0)
BstEII 174 GGTGACC G^GT[ACGT]ACC
BstPI 174 GGTGACC G^GT[ACGT]ACC
CelII 1177 GCTAAGC GC^T[ACGT]AGC
EarI 790 GAAGAG CTCTTC(1/4)
EcoO65I 174 GGTGACC G^GT[ACGT]ACC
EcoRI 446 GAATTC G^AATTC
FauI 1114 CCCGC CCCGC(4/6)
FbaI 338 TGATCA T^GATCA
Ksp632I 790 GAAGAG CTCTTC(1/4)
MroI 1403 TCCGGA T^CCGGA
NspI 1569 GCATGT [GA]CATG^[CT]
PleI 1407 GACTC GAGTC(4/5)
RsaI 1330 GTAC GT^AC
SapI 790 GAAGAGC GCTCTTC(1/4)
StuI 147 AGGCCT AGG^CCT
TaqI 379 TCGA T^CGA
TfiI 360 GATTC G^A[AT]TC
TthHB8I 379 TCGA T^CGA
XmnI 405 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 727 GGCGCC G[GA]^CG[CT]C
1285 GGCGCC
AlwI 249 GGATC GGATC(4/5)
713 GGATC
AspHI 25 GTGCTC G[AT]GC[AT]^C
613 GTGCAC
BalI 350 TGGCCA TGG^CCA
753 TGGCCA
BanII 740 GGGCCC G[GA]GC[CT]^C
1339 GGGCTC
BbeI 727 GGCGCC GGCGC^C
1285 GGCGCC
BbsI 507 GAAGAC GAAGAC(2/6)
1354 GTCTTC
BglII 62 AGATCT A^GATCT
584 AGATCT
BpuAI 507 GAAGAC GAAGAC(2/6)
1354 GTCTTC
BsaHI 727 GGCGCC G[GA]^CG[CT]C
1285 GGCGCC
BsaI 1510 GAGACC GGTCTC(1/5)
543 GGTCTC
BsgI 770 CTGCAC GTGCAG(16/14)
1232 CTGCAC
BsiHKAI 25 GTGCTC G[AT]GC[AT]^C
613 GTGCAC
BsmAI 1510 GAGAC GTCTC(1/5)
544 GTCTC
BspMI 1385 ACCTGC ACCTGC(4/8)
84 GCAGGT
CfoI 728 GCGC GCG^C
1286 GCGC
EcoNI 1134 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1452 CCTGGCAGAGG
HaeII 727 GGCGCC [GA]GCGC^[CT]
1285 GGCGCC
HhaI 728 GCGC GCG^C
1286 GCGC
Hin1I 727 GGCGCC G[GA]^CG[CT]C
1285 GGCGCC
HinP1I 728 GCGC G^CGC
1286 GCGC
HinfI 360 GATTC G^A[ACGT]TC
1407 GACTC
KasI 727 GGCGCC G^GCGCC
1285 GGCGCC
MaeII 779 ACGT A^CGT
1520 ACGT
MluNI 350 TGGCCA TGG^CCA
753 TGGCCA
MscI 350 TGGCCA TGG^CCA
753 TGGCCA
NarI 727 GGCGCC GG^CGCC
1285 GGCGCC
PpuMI 1042 GGGTCCC [GA]G^G[AT]CC[CT]
1382 GGGACCT
SfoI 727 GGCGCC GGC^GCC
1285 GGCGCC
Tsp509I 447 AATT ^AATT
894 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AluI AvaII BanI BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI Van91I XhoII