################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BciVI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiWI Bsp1407I BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcyI 582 GGCGTC G[GA]^CG[CT]C AflIII 950 ACGTGT A^C[GA][CT]GT Alw44I 374 GTGCAC G^TGCAC AlwI 410 GGATC GGATC(4/5) ApaI 401 GGGCCC GGGCC^C ApaLI 374 GTGCAC G^TGCAC BbsI 700 GTCTTC GAAGAC(2/6) BbvCI 537 CCTCAGC CCTCAGC(-5/-2) BcgI 230 GCACCAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1427 TGATCA T^GATCA BglI 455 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1116 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1116 GCTCAGC GC^T[ACGT]AGC BpuAI 700 GTCTTC GAAGAC(2/6) BsaHI 582 GGCGTC G[GA]^CG[CT]C BsmI 1038 GAATGC GAATGC(1/-1) BspDI 1093 ATCGAT AT^CGAT BspHI 1508 TCATGA T^CATGA BspMI 1052 ACCTGC ACCTGC(4/8) BsrBI 1541 GAGCGG CCGCTC(-3/-3) BsrFI 339 GCCGGC [GA]^CCGG[CT] BstAPI 859 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1116 GCTCAGC GC^T[ACGT]AGC Cfr10I 339 GCCGGC [GA]^CCGG[CT] ClaI 1093 ATCGAT AT^CGAT FbaI 1427 TGATCA T^GATCA Hin1I 582 GGCGTC G[GA]^CG[CT]C MaeIII 295 GTGAC ^GT[ACGT]AC MslI 641 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 339 GCCGGC GCC^GGC NgoMIV 339 GCCGGC G^CCGGC PflMI 723 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1508 TCATGA T^CATGA SapI 600 GCTCTTC GCTCTTC(1/4) SbfI 856 CCTGCAGG CCTGCA^GG SmlI 976 CTCAAG C^T[CT][GA]AG Sse8387I 856 CCTGCAGG CCTGCA^GG Tsp45I 295 GTGAC ^GT[GC]AC Van91I 723 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccII 216 CGCG CG^CG 501 CGCG BfaI 31 CTAG C^TAG 1453 CTAG BsaI 673 GAGACC GGTCTC(1/5) 1063 GAGACC BsrDI 741 CATTGC GCAATG(2/0) 1548 CATTGC BstUI 216 CGCG CG^CG 501 CGCG DrdI 145 GACTCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1155 GACAGGCACGTC EarI 601 CTCTTC CTCTTC(1/4) 1734 GAAGAG FauI 1463 GCGGG CCCGC(4/6) 1472 GCGGG HaeII 54 GGCGCT [GA]GCGC^[CT] 1195 AGCGCC Ksp632I 601 CTCTTC CTCTTC(1/4) 1734 GAAGAG MaeI 31 CTAG C^TAG 1453 CTAG MspA1I 481 CAGCTG C[AC]G^C[GT]G 760 CAGCTG MvnI 216 CGCG CG^CG 501 CGCG NspI 519 GCATGT [GA]CATG^[CT] 1566 GCATGT PstI 769 CTGCAG CTGCA^G 857 CTGCAG PvuII 481 CAGCTG CAG^CTG 760 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AfaI AluI AlwNI AocI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeII MboI MboII MluNI MnlI MscI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI TseI TspRI TthHB8I XcmI XmaCI XmaI