Restriction map for
pF1KB4992
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# NO CUT ENZYMES ( 138 enzymes) #
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Acc65I AccI AclI AflII AflIII
AgeI AhdI AlwI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BlnI BsaAI BsaBI
BsaI BseRI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FseI FspI HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MscI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 480 GACGTC GACGT^C
AccII 386 CGCG CG^CG
AccIII 551 TCCGGA T^CCGGA
AcyI 480 GACGTC G[GA]^CG[CT]C
Alw44I 166 GTGCAC G^TGCAC
AocI 793 CCTGAGG CC^T[ACGT]AGG
ApaLI 166 GTGCAC G^TGCAC
BanII 700 GAGCTC G[GA]GC[CT]^C
BbsI 826 GAAGAC GAAGAC(2/6)
BclI 426 TGATCA T^GATCA
BcnI 157 CCCGG CC^[GC]GG
BfaI 199 CTAG C^TAG
BglII 431 AGATCT A^GATCT
BlpI 522 GCTGAGC GC^T[ACGT]AGC
BpmI 655 CTGGAG CTGGAG(16/14)
Bpu1102I 522 GCTGAGC GC^T[ACGT]AGC
BpuAI 826 GAAGAC GAAGAC(2/6)
BsaHI 480 GACGTC G[GA]^CG[CT]C
BsaWI 551 TCCGGA [AT]^CCGG[AT]
BseAI 551 TCCGGA T^CCGGA
BsgI 82 CTGCAC GTGCAG(16/14)
BsmAI 589 GAGAC GTCTC(1/5)
BsmFI 483 GTCCC GGGAC(10/14)
BsmI 545 GCATTC GAATGC(1/-1)
BspEI 551 TCCGGA T^CCGGA
BsrDI 527 GCAATG GCAATG(2/0)
BsrFI 518 GCCGGC [GA]^CCGG[CT]
BstUI 386 CGCG CG^CG
BstYI 431 AGATCT [GA]^GATC[CT]
Bsu36I 793 CCTGAGG CC^T[ACGT]AGG
CelII 522 GCTGAGC GC^T[ACGT]AGC
Cfr10I 518 GCCGGC [GA]^CCGG[CT]
Eco81I 793 CCTGAGG CC^T[ACGT]AGG
EcoNI 415 CCTAACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 426 TGATCA T^GATCA
HaeII 405 AGCGCC [GA]GCGC^[CT]
HgaI 346 GACGC GACGC(5/10)
Hin1I 480 GACGTC G[GA]^CG[CT]C
HphI 424 GGTGA GGTGA(8/7)
MaeI 199 CTAG C^TAG
MflI 431 AGATCT [GA]^GATC[CT]
MroI 551 TCCGGA T^CCGGA
MseI 332 TTAA T^TAA
MspA1I 622 CAGCTG C[AC]G^C[GT]G
MvnI 386 CGCG CG^CG
NaeI 518 GCCGGC GCC^GGC
NciI 157 CCCGG CC^[GC]GG
NgoMIV 518 GCCGGC G^CCGGC
NlaIV 534 GGCCCC GG[ACGT]^[ACGT]CC
PvuII 622 CAGCTG CAG^CTG
SacI 700 GAGCTC GAGCT^C
SfcI 275 CTGTAG C^T[GA][CT]AG
SmlI 171 CTTGAG C^T[CT][GA]AG
TaqI 698 TCGA T^CGA
Tru9I 332 TTAA T^TAA
TthHB8I 698 TCGA T^CGA
XhoII 431 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 387 GCGG CCGC(-3/-1)
581 GCGG
AfaI 15 GTAC GT^AC
840 GTAC
AspHI 166 GTGCAC G[AT]GC[AT]^C
700 GAGCTC
BmyI 166 GTGCAC G[AGT]GC[ACT]^C
700 GAGCTC
BsaJI 156 CCCCGG C^C[ACGT][ACGT]GG
420 CCCAGG
BsiHKAI 166 GTGCAC G[AT]GC[AT]^C
700 GAGCTC
Bsp1286I 166 GTGCAC G[AGT]GC[ACT]^C
700 GAGCTC
BsrI 32 CCAGT ACTGG(1/-1)
135 CCAGT
CfoI 385 GCGC GCG^C
406 GCGC
DpnII 427 GATC ^GATC
432 GATC
EarI 151 CTCTTC CTCTTC(1/4)
811 GAAGAG
HhaI 385 GCGC GCG^C
406 GCGC
HinP1I 385 GCGC G^CGC
406 GCGC
HincII 70 GTCAAC GT[CT]^[GA]AC
259 GTTGAC
HindII 70 GTCAAC GT[CT]^[GA]AC
259 GTTGAC
Ksp632I 151 CTCTTC CTCTTC(1/4)
811 GAAGAG
MboI 427 GATC ^GATC
432 GATC
NdeII 427 GATC ^GATC
432 GATC
NspI 146 ACATGC [GA]CATG^[CT]
503 ACATGC
PflMI 135 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
618 CCAACAGCTGG
PleI 76 GACTC GAGTC(4/5)
45 GAGTC
RsaI 15 GTAC GT^AC
840 GTAC
SapI 811 GAAGAGC GCTCTTC(1/4)
150 GCTCTTC
Sau3AI 427 GATC ^GATC
432 GATC
TfiI 558 GAATC G^A[AT]TC
782 GATTC
Van91I 135 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
618 CCAACAGCTGG
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsiYI
BslI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EaeI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MaeII MaeIII MboII MnlI
MslI MspI MvaI MwoI NlaIII
Sau96I ScrFI SfaNI TseI Tsp45I
Tsp509I TspRI