Restriction map for
pF1KB4753
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI AlwI AlwNI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BsrGI BsrI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HaeIII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MboII MfeI MflI MluI MluNI
MroI MscI MseI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AfaI 126 GTAC GT^AC
AflIII 77 ACACGT A^C[GA][CT]GT
Alw44I 91 GTGCAC G^TGCAC
ApaLI 91 GTGCAC G^TGCAC
AspHI 91 GTGCAC G[AT]GC[AT]^C
BbrPI 78 CACGTG CAC^GTG
BcnI 16 CCCGG CC^[GC]GG
BpmI 315 CTGGAG CTGGAG(16/14)
BseRI 43 GAGGAG GAGGAG(10/8)
BsiHKAI 91 GTGCAC G[AT]GC[AT]^C
BsrBI 252 CCGCTC CCGCTC(-3/-3)
BsrDI 293 GCAATG GCAATG(2/0)
BssSI 224 CTCGTG CACGAG(-5/-1)
BstAPI 74 GCAACACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 173 CCTGG CC^[AT]GG
Cac8I 70 GCCTGC GC[ACGT]^[ACGT]GC
DdeI 299 CTCAG C^T[ACGT]AG
DpnII 36 GATC ^GATC
EcoRII 173 CCTGG ^CC[AT]GG
HapII 17 CCGG C^CGG
HincII 184 GTTGAC GT[CT]^[GA]AC
HindII 184 GTTGAC GT[CT]^[GA]AC
HpaII 17 CCGG C^CGG
HphI 101 TCACC GGTGA(8/7)
MaeIII 112 GTAAC ^GT[ACGT]AC
MboI 36 GATC ^GATC
MslI 220 CATCCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 17 CCGG C^CGG
MvaI 173 CCTGG CC^[AT]GG
NciI 16 CCCGG CC^[GC]GG
NdeII 36 GATC ^GATC
NlaIII 237 CATG CATG^
PmaCI 78 CACGTG CAC^GTG
PmlI 78 CACGTG CAC^GTG
PvuII 167 CAGCTG CAG^CTG
RsaI 126 GTAC GT^AC
Sau3AI 36 GATC ^GATC
TspRI 301 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 16 enzymes) #
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AluI 168 AGCT AG^CT
242 AGCT
BmyI 81 GTGCCC G[AGT]GC[ACT]^C
91 GTGCAC
BsaAI 78 CACGTG [CT]AC^GT[GA]
88 TACGTG
BsaJI 15 CCCCGG C^C[ACGT][ACGT]GG
173 CCTGGG
BsmFI 48 GGGAC GGGAC(10/14)
206 GTCCC
Bsp1286I 81 GTGCCC G[AGT]GC[ACT]^C
91 GTGCAC
BssKI 16 CCCGG ^CC[ACGT]GG
173 CCTGG
BstF5I 220 CATCC GGATG(2/0)
147 GGATG
FokI 220 CATCC GGATG(9/13)
147 GGATG
HinfI 187 GACTC G^A[ACGT]TC
199 GAGTC
MaeII 79 ACGT A^CGT
89 ACGT
MspA1I 167 CAGCTG C[AC]G^C[GT]G
312 CCGCTG
PleI 187 GACTC GAGTC(4/5)
199 GAGTC
PstI 194 CTGCAG CTGCA^G
257 CTGCAG
ScrFI 16 CCCGG CC^[ACGT]GG
173 CCTGG
SfcI 194 CTGCAG C^T[GA][CT]AG
257 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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AciI BbvI BsiYI BslI FauI
Fnu4HI ItaI MnlI MwoI TseI