################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BbvI BcgI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HindIII HinfI HpaI HphI KasI KpnI KspI MamI MluI MluNI MscI MspA1I NarI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI StuI StyI SwaI TfiI TseI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccI 897 GTCTAC GT^[AC][GT]AC AccII 45 CGCG CG^CG AcsI 289 AAATTC [GA]^AATT[CT] AcyI 670 GGCGTC G[GA]^CG[CT]C AluI 705 AGCT AG^CT Aor51HI 7 AGCGCT AGC^GCT ApoI 289 AAATTC [GA]^AATT[CT] AseI 478 ATTAAT AT^TAAT AsnI 478 ATTAAT AT^TAAT AvaI 138 CCCGGG C^[CT]CG[GA]G BanI 49 GGCACC G^G[CT][GA]CC BclI 458 TGATCA T^GATCA BfaI 64 CTAG C^TAG BsaHI 670 GGCGTC G[GA]^CG[CT]C BseRI 652 GAGGAG GAGGAG(10/8) BsgI 679 GTGCAG GTGCAG(16/14) BsmBI 757 GAGACG CGTCTC(1/5) BsmFI 586 GTCCC GGGAC(10/14) BsoBI 138 CCCGGG C^[CT]CG[GA]G BspHI 881 TCATGA T^CATGA BspMI 851 ACCTGC ACCTGC(4/8) BsrDI 312 CATTGC GCAATG(2/0) BsrFI 22 GCCGGC [GA]^CCGG[CT] BstF5I 435 GGATG GGATG(2/0) BstUI 45 CGCG CG^CG BstYI 535 AGATCC [GA]^GATC[CT] Cfr10I 22 GCCGGC [GA]^CCGG[CT] DsaI 53 CCGTGG C^C[GA][CT]GG Eco47III 7 AGCGCT AGC^GCT EcoNI 645 CCTCCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 458 TGATCA T^GATCA Fnu4HI 19 GCCGC GC^[ACGT]GC FokI 435 GGATG GGATG(9/13) HaeII 7 AGCGCT [GA]GCGC^[CT] HgaI 671 GCGTC GACGC(5/10) Hin1I 670 GGCGTC G[GA]^CG[CT]C HincII 319 GTTGAC GT[CT]^[GA]AC HindII 319 GTTGAC GT[CT]^[GA]AC ItaI 19 GCCGC GC^[ACGT]GC MaeI 64 CTAG C^TAG MaeIII 163 GTCAC ^GT[ACGT]AC MfeI 508 CAATTG C^AATTG MflI 535 AGATCC [GA]^GATC[CT] MunI 508 CAATTG C^AATTG MvnI 45 CGCG CG^CG NaeI 22 GCCGGC GCC^GGC NdeI 194 CATATG CA^TATG NgoMIV 22 GCCGGC G^CCGGC NlaIV 49 GGCACC GG[ACGT]^[ACGT]CC NspI 575 GCATGC [GA]CATG^[CT] PshBI 478 ATTAAT AT^TAAT RcaI 881 TCATGA T^CATGA SapI 87 GCTCTTC GCTCTTC(1/4) ScaI 172 AGTACT AGT^ACT SexAI 263 ACCAGGT A^CC[AT]GGT SmaI 138 CCCGGG CCC^GGG SphI 575 GCATGC GCATG^C TaqI 838 TCGA T^CGA Tsp45I 163 GTCAC ^GT[GC]AC TthHB8I 838 TCGA T^CGA XhoII 535 AGATCC [GA]^GATC[CT] XmaCI 138 CCCGGG C^CCGGG XmaI 138 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccIII 725 TCCGGA T^CCGGA 753 TCCGGA AlwI 536 GATCC GGATC(4/5) 819 GATCC BciVI 811 GGATAC GTATCC(6/5) 417 GTATCC BsaWI 725 TCCGGA [AT]^CCGG[AT] 753 TCCGGA BseAI 725 TCCGGA T^CCGGA 753 TCCGGA BsmAI 301 GAGAC GTCTC(1/5) 757 GAGAC BspEI 725 TCCGGA T^CCGGA 753 TCCGGA BstNI 264 CCAGG CC^[AT]GG 520 CCTGG CfoI 8 GCGC GCG^C 44 GCGC Cfr13I 96 GGCCC G^G[ACGT]CC 136 GGCCC DdeI 387 CTTAG C^T[ACGT]AG 638 CTCAG EarI 88 CTCTTC CTCTTC(1/4) 202 GAAGAG EcoRII 264 CCAGG ^CC[AT]GG 520 CCTGG HhaI 8 GCGC GCG^C 44 GCGC HinP1I 8 GCGC G^CGC 44 GCGC Ksp632I 88 CTCTTC CTCTTC(1/4) 202 GAAGAG MaeII 398 ACGT A^CGT 760 ACGT MroI 725 TCCGGA T^CCGGA 753 TCCGGA MslI 384 CATCTTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 882 CATGAATATG MvaI 264 CCAGG CC^[AT]GG 520 CCTGG MwoI 10 GCTCTCCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 58 GCCTACCTAGC Sau96I 96 GGCCC G^G[ACGT]CC 136 GGCCC SfaNI 328 GCATC GCATC(5/9) 383 GCATC SspI 475 AATATT AAT^ATT 481 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AciI AfaI BanII BcnI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI Cac8I DpnII FauI HaeIII HapII HpaII MboI MboII MnlI MseI MspI NciI NdeII NlaIII RsaI Sau3AI ScrFI Tru9I Tsp509I TspRI