Restriction map for
pF1KE2684
##################################
# NO CUT ENZYMES ( 190 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaJI BseAI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeIII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
TseI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 544 GTATAC GT^[AC][GT]AC
AfaI 225 GTAC GT^AC
BanII 218 GGGCTC G[GA]GC[CT]^C
BbsI 208 GAAGAC GAAGAC(2/6)
BpuAI 208 GAAGAC GAAGAC(2/6)
BsaWI 15 ACCGGA [AT]^CCGG[AT]
BseRI 55 GAGGAG GAGGAG(10/8)
BsmBI 363 GAGACG CGTCTC(1/5)
BsmFI 594 GTCCC GGGAC(10/14)
Bsp1407I 224 TGTACA T^GTACA
BspMI 176 ACCTGC ACCTGC(4/8)
BsrBI 618 GAGCGG CCGCTC(-3/-3)
BsrGI 224 TGTACA T^GTACA
BssKI 259 CCTGG ^CC[ACGT]GG
Bst1107I 544 GTATAC GTA^TAC
BstNI 259 CCTGG CC^[AT]GG
BstZ17I 544 GTATAC GTA^TAC
Cac8I 168 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 452 GCGC GCG^C
DdeI 614 CTAAG C^T[ACGT]AG
DpnII 186 GATC ^GATC
DraI 142 TTTAAA TTT^AAA
EarI 52 GAAGAG CTCTTC(1/4)
EcoRII 259 CCTGG ^CC[AT]GG
HaeII 451 GGCGCT [GA]GCGC^[CT]
HapII 16 CCGG C^CGG
HhaI 452 GCGC GCG^C
HinP1I 452 GCGC G^CGC
HpaII 16 CCGG C^CGG
HphI 478 GGTGA GGTGA(8/7)
Ksp632I 52 GAAGAG CTCTTC(1/4)
MboI 186 GATC ^GATC
MslI 398 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 238 CAGCTG C[AC]G^C[GT]G
MspI 16 CCGG C^CGG
MvaI 259 CCTGG CC^[AT]GG
MwoI 601 GCAGACACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 186 GATC ^GATC
NlaIV 622 GGCTCC GG[ACGT]^[ACGT]CC
PvuII 238 CAGCTG CAG^CTG
RsaI 225 GTAC GT^AC
Sau3AI 186 GATC ^GATC
ScrFI 259 CCTGG CC^[ACGT]GG
SspBI 224 TGTACA T^GTACA
TaqI 11 TCGA T^CGA
TspRI 435 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 11 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 180 GCGG CCGC(-3/-1)
620 GCGG
BfaI 245 CTAG C^TAG
391 CTAG
BmyI 128 GGGCAC G[AGT]GC[ACT]^C
218 GGGCTC
BsmAI 363 GAGAC GTCTC(1/5)
394 GAGAC
Bsp1286I 128 GGGCAC G[AGT]GC[ACT]^C
218 GGGCTC
BstF5I 109 CATCC GGATG(2/0)
285 GGATG
Fnu4HI 180 GCGGC GC^[ACGT]GC
620 GCGGC
FokI 109 CATCC GGATG(9/13)
285 GGATG
HinfI 67 GAATC G^A[ACGT]TC
418 GAATC
ItaI 180 GCGGC GC^[ACGT]GC
620 GCGGC
MaeI 245 CTAG C^TAG
391 CTAG
TfiI 67 GAATC G^A[AT]TC
418 GAATC
Tsp45I 266 GTGAC ^GT[GC]AC
371 GTGAC
Tsp509I 250 AATT ^AATT
342 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
AluI MaeIII MboII MnlI MseI
SfcI Tru9I