Restriction map for
pF1KB6437
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BclI BfrI BglII
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII KasI KpnI KspI
MaeII MamI MluI MluNI MroI
MscI MspA1I MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I StuI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 340 GTCTAC GT^[AC][GT]AC
AfaI 766 GTAC GT^AC
Alw44I 650 GTGCAC G^TGCAC
ApaLI 650 GTGCAC G^TGCAC
AspHI 650 GTGCAC G[AT]GC[AT]^C
AvaII 108 GGACC G^G[AT]CC
AvrII 870 CCTAGG C^CTAGG
BanII 29 GAGCCC G[GA]GC[CT]^C
BciVI 3 GGATAC GTATCC(6/5)
BcnI 319 CCGGG CC^[GC]GG
BglI 68 GCCTAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 870 CCTAGG C^CTAGG
BpmI 262 CTGGAG CTGGAG(16/14)
BsaI 420 GGTCTC GGTCTC(1/5)
BseRI 551 CTCCTC GAGGAG(10/8)
BsiHKAI 650 GTGCAC G[AT]GC[AT]^C
BsmAI 421 GTCTC GTCTC(1/5)
BsmFI 53 GTCCC GGGAC(10/14)
BsmI 292 GAATGC GAATGC(1/-1)
Bsp1407I 765 TGTACA T^GTACA
BsrGI 765 TGTACA T^GTACA
BstNI 261 CCTGG CC^[AT]GG
BstYI 855 GGATCT [GA]^GATC[CT]
Cac8I 77 GCATGC GC[ACGT]^[ACGT]GC
Cfr13I 108 GGACC G^G[ACGT]CC
DraI 283 TTTAAA TTT^AAA
DsaI 146 CCATGG C^C[GA][CT]GG
EarI 46 GAAGAG CTCTTC(1/4)
EcoRI 323 GAATTC G^AATTC
EcoRII 261 CCTGG ^CC[AT]GG
HaeIII 663 GGCC GG^CC
HapII 319 CCGG C^CGG
HpaI 250 GTTAAC GTT^AAC
HpaII 319 CCGG C^CGG
Ksp632I 46 GAAGAG CTCTTC(1/4)
MaeIII 903 GTCAC ^GT[ACGT]AC
MfeI 824 CAATTG C^AATTG
MflI 855 GGATCT [GA]^GATC[CT]
MspI 319 CCGG C^CGG
MunI 824 CAATTG C^AATTG
MvaI 261 CCTGG CC^[AT]GG
NciI 319 CCGGG CC^[GC]GG
NcoI 146 CCATGG C^CATGG
PstI 566 CTGCAG CTGCA^G
RsaI 766 GTAC GT^AC
Sau96I 108 GGACC G^G[ACGT]CC
SphI 77 GCATGC GCATG^C
SspBI 765 TGTACA T^GTACA
SspI 235 AATATT AAT^ATT
Tsp45I 903 GTCAC ^GT[GC]AC
XhoII 855 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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BfaI 859 CTAG C^TAG
871 CTAG
BmyI 29 GAGCCC G[AGT]GC[ACT]^C
650 GTGCAC
BsaJI 146 CCATGG C^C[ACGT][ACGT]GG
870 CCTAGG
BsiYI 261 CCTGGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
313 CCTTCTCCGGG
BslI 261 CCTGGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
313 CCTTCTCCGGG
Bsp1286I 29 GAGCCC G[AGT]GC[ACT]^C
650 GTGCAC
BssKI 261 CCTGG ^CC[ACGT]GG
319 CCGGG
BstF5I 100 CATCC GGATG(2/0)
534 GGATG
EcoT14I 146 CCATGG C^C[AT][AT]GG
870 CCTAGG
FokI 100 CATCC GGATG(9/13)
534 GGATG
HincII 250 GTTAAC GT[CT]^[GA]AC
384 GTCAAC
HindII 250 GTTAAC GT[CT]^[GA]AC
384 GTCAAC
HinfI 835 GACTC G^A[ACGT]TC
901 GAGTC
HphI 370 TCACC GGTGA(8/7)
459 TCACC
MaeI 859 CTAG C^TAG
871 CTAG
MslI 147 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
529 CACACGGATG
MwoI 59 GCACTTATAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
68 GCCTAACTGGC
NlaIV 28 GGAGCC GG[ACGT]^[ACGT]CC
108 GGACCC
NspI 77 GCATGC [GA]CATG^[CT]
336 GCATGT
PleI 835 GACTC GAGTC(4/5)
901 GAGTC
ScrFI 261 CCTGG CC^[ACGT]GG
319 CCGGG
StyI 146 CCATGG C^C[AT][AT]GG
870 CCTAGG
TspRI 104 CACTG CA[GC]TG[ACGT][ACGT]^
653 CACTG
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BsrI DdeI DpnII Fnu4HI ItaI
MboI MboII MnlI MseI NdeII
NlaIII Sau3AI SfaNI SfcI TaqI
Tru9I TseI Tsp509I TthHB8I