Restriction map for
pF1KB5957
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspI AvrII BamHI BbeI
BbrPI BciVI BfrI BglII BlnI
BsaAI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstYI
BstZ17I Bsu36I ClaI CpoI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FokI FseI HaeII HgaI HincII
HindII HindIII HpaI KasI KpnI
MfeI MflI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI StuI SwaI Tth111I
XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 476 GACGTC GACGT^C
AcyI 476 GACGTC G[GA]^CG[CT]C
AflIII 149 ACGTGT A^C[GA][CT]GT
AhdI 861 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1033 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1209 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 861 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 242 TGCGCA TGC^GCA
BbsI 1165 GAAGAC GAAGAC(2/6)
BclI 1460 TGATCA T^GATCA
BlpI 700 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 700 GCTCAGC GC^T[ACGT]AGC
BpuAI 1165 GAAGAC GAAGAC(2/6)
BsaBI 333 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 476 GACGTC G[GA]^CG[CT]C
BsmBI 474 GAGACG CGTCTC(1/5)
BsmFI 755 GGGAC GGGAC(10/14)
BsrFI 203 ACCGGC [GA]^CCGG[CT]
BssSI 500 CACGAG CACGAG(-5/-1)
BstXI 338 CCATCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 700 GCTCAGC GC^T[ACGT]AGC
Cfr10I 203 ACCGGC [GA]^CCGG[CT]
DraIII 379 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 144 GACTAACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 253 CGGCCG C^GGCCG
Eam1105I 861 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 253 CGGCCG C^GGCCG
Eco52I 253 CGGCCG C^GGCCG
FbaI 1460 TGATCA T^GATCA
FspI 242 TGCGCA TGC^GCA
Hin1I 476 GACGTC G[GA]^CG[CT]C
KspI 372 CCGCGG CCGC^GG
MamI 333 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 339 CATCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1523 CCATGG C^CATGG
NspI 191 ACATGC [GA]CATG^[CT]
PaeR7I 394 CTCGAG C^TCGAG
PflMI 1299 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1034 AGGACCT [GA]G^G[AT]CC[CT]
SacI 736 GAGCTC GAGCT^C
SacII 372 CCGCGG CCGC^GG
SbfI 741 CCTGCAGG CCTGCA^GG
ScaI 62 AGTACT AGT^ACT
Sse8387I 741 CCTGCAGG CCTGCA^GG
SspI 1247 AATATT AAT^ATT
Tsp509I 920 AATT ^AATT
Van91I 1299 CCAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 200 CCAACCGGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 394 CTCGAG C^TCGAG
XmnI 1209 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 987 GATCC GGATC(4/5)
800 GGATC
ApaI 262 GGGCCC GGGCC^C
482 GGGCCC
AspHI 367 GAGCAC G[AT]GC[AT]^C
736 GAGCTC
BanI 206 GGCACC G^G[CT][GA]CC
649 GGTGCC
BbvCI 684 CCTCAGC CCTCAGC(-5/-2)
1196 CCTCAGC
BcgI 585 GCATGAGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
689 GCAGCCTGATCG
BfaI 619 CTAG C^TAG
706 CTAG
BpmI 809 CTCCAG CTGGAG(16/14)
1297 CTCCAG
BsgI 1176 GTGCAG GTGCAG(16/14)
1448 GTGCAG
BsiEI 253 CGGCCG CG[GA][CT]^CG
354 CGACCG
BsiHKAI 367 GAGCAC G[AT]GC[AT]^C
736 GAGCTC
BspMI 43 ACCTGC ACCTGC(4/8)
1037 ACCTGC
BsrI 228 ACTGG ACTGG(1/-1)
660 CCAGT
CfoI 110 GCGC GCG^C
243 GCGC
DraI 447 TTTAAA TTT^AAA
465 TTTAAA
DraII 261 GGGGCCC [GA]G^G[ACGT]CC[CT]
1034 AGGACCT
DsaI 372 CCGCGG C^C[GA][CT]GG
1523 CCATGG
EarI 471 GAAGAG CTCTTC(1/4)
709 GAAGAG
EcoNI 27 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1026 CCTCCAACAGG
EcoO109I 261 GGGGCCC [GA]G^G[ACGT]CC[CT]
1034 AGGACCT
EcoT14I 796 CCTTGG C^C[AT][AT]GG
1523 CCATGG
HhaI 110 GCGC GCG^C
243 GCGC
HinP1I 110 GCGC G^CGC
243 GCGC
HphI 1333 GGTGA GGTGA(8/7)
656 TCACC
Ksp632I 471 GAAGAG CTCTTC(1/4)
709 GAAGAG
MaeI 619 CTAG C^TAG
706 CTAG
PvuII 238 CAGCTG CAG^CTG
562 CAGCTG
SfiI 254 GGCCGTCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1403 GGCCTGTCCGGCC
StyI 796 CCTTGG C^C[AT][AT]GG
1523 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BalI BanII BbvI BcnI
BglI BmyI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrBI BssKI BstNI BstUI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
FauI Fnu4HI HaeIII HapII HinfI
HpaII ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I