Restriction map for
pF1KB4740
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# NO CUT ENZYMES ( 101 enzymes) #
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AccI AccIII AclI AcsI AflII
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspI
AvrII BbeI BbvCI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KasI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 759 GACGTC GACGT^C
Acc65I 2398 GGTACC G^GTACC
AccII 1086 CGCG CG^CG
AcyI 759 GACGTC G[GA]^CG[CT]C
AflIII 942 ACGTGT A^C[GA][CT]GT
AgeI 2395 ACCGGT A^CCGGT
AhdI 1927 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1212 GGGCCC GGGCC^C
Asp718I 2398 GGTACC G^GTACC
AspEI 1927 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1097 TGCGCA TGC^GCA
BalI 689 TGGCCA TGG^CCA
BbrPI 1540 CACGTG CAC^GTG
BcgI 1826 GCAGATTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 759 GACGTC G[GA]^CG[CT]C
BsaWI 2395 ACCGGT [AT]^CCGG[AT]
BsgI 773 CTGCAC GTGCAG(16/14)
BsiEI 1082 CGACCG CG[GA][CT]^CG
BspHI 1595 TCATGA T^CATGA
BsrBI 985 GAGCGG CCGCTC(-3/-3)
BstAPI 548 GCACAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1573 TTCGAA TT^CGAA
BstUI 1086 CGCG CG^CG
DraI 246 TTTAAA TTT^AAA
DraIII 1705 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 689 TGGCCA [CT]^GGCC[GA]
Eam1105I 1927 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1097 TGCGCA TGC^GCA
HaeII 1842 GGCGCT [GA]GCGC^[CT]
Hin1I 759 GACGTC G[GA]^CG[CT]C
KpnI 2398 GGTACC GGTAC^C
KspI 1085 CCGCGG CCGC^GG
MluNI 689 TGGCCA TGG^CCA
MscI 689 TGGCCA TGG^CCA
MvnI 1086 CGCG CG^CG
NspV 1573 TTCGAA TT^CGAA
PinAI 2395 ACCGGT A^CCGGT
PmaCI 1540 CACGTG CAC^GTG
PmlI 1540 CACGTG CAC^GTG
RcaI 1595 TCATGA T^CATGA
SacI 183 GAGCTC GAGCT^C
SacII 1085 CCGCGG CCGC^GG
SfuI 1573 TTCGAA TT^CGAA
SmaI 1428 CCCGGG CCC^GGG
SphI 2252 GCATGC GCATG^C
XcmI 2162 CCAAAGCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1428 CCCGGG C^CCGGG
XmaI 1428 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwNI 722 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
878 CAGCGGCTG
BanII 183 GAGCTC G[GA]GC[CT]^C
1212 GGGCCC
BbsI 162 GAAGAC GAAGAC(2/6)
1179 GTCTTC
BglI 677 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
826 GCCACACCGGC
BpuAI 162 GAAGAC GAAGAC(2/6)
1179 GTCTTC
BsmFI 1444 GGGAC GGGAC(10/14)
762 GTCCC
BssSI 1705 CACGAG CACGAG(-5/-1)
2053 CACGAG
NaeI 647 GCCGGC GCC^GGC
1625 GCCGGC
NcoI 1838 CCATGG C^CATGG
1851 CCATGG
NgoMIV 647 GCCGGC G^CCGGC
1625 GCCGGC
NspI 347 ACATGC [GA]CATG^[CT]
2252 GCATGC
SapI 2152 GAAGAGC GCTCTTC(1/4)
2283 GAAGAGC
SmlI 270 CTTGAG C^T[CT][GA]AG
780 CTTGAG
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BamHI BanI BbvI
BcnI BfaI BmyI BpmI BsaAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII